Improved early development potence of in vitro fertilization embryos by treatment with tubacin increasing acetylated tubulin of matured porcine oocytes

Cui, Kuiqing; Li, Zhi Peng; Gao, Bangjun; Huang, Bingqing; Liu, QingYou; Shi, Dongquan

doi:10.1016/j.mod.2020.103631

Cited by 7 publications

(4 citation statements)

References 34 publications

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“…Among them, VASH2 was belonged to the Vasohibin family, which is involved in angiogenesis [41,42] and veri ed as a biomarker for the esophageal squamous cell carcinoma [43]. As a signi cant component of microtubules, tubulin is involved in various biological processes through posttranslational modi cation, including early embryo development, cytoskeletal maintenance, and tuberculosis and tumor-related diseases [44,45]. However, the mechanisms by which these genes affect embryo muscle development are unknown, which necessitates future exploration.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Embryonic Muscle Development in Chengkou Mountain Chicken

Ren

Liu

Wang

et al. 2020

Preprint

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Background: Muscle is the predominant portion of any meat product, and growth performance and product quality are the core of modern breeding. The embryonic period is highly critical for muscle development, the number, shape and structure of muscle fibers are determined at the embryonic stage. Herein, we performed transcriptome analysis to reveal the law of muscle development in the embryonic stage of Chengkou Mountain Chicken at embryonic days (E) 12, 16, 19, 21. Results: Diameter and area of muscle fibers exhibited significant difference at different embryonic times(P<0.01). A total of 16,330 mRNAs transcripts were detected, including 109 novel mRNAs transcripts. By comparing different embryonic muscle development time points, 2,251 (E12vsE16), 4,324 (E12vsE19), and 5,224 (E12vsE21), 1,274 (E16vsE19), 2,735 (E16vsE21) and 857 (E19vsE21) differentially expressed mRNAs were identified. It is worth noting that 6,726 mRNAs were differentially expressed. The time-series expression profile of differentially expressed genes (DEGs) showed that the rising and falling expression trends were significantly enriched. The downward trend was the most important and was enriched in 3,963 DEGs. GO enrichment analysis provided three significantly enriched categories of the down-trending genes, including 91 cellular components, 53 molecular functions, and 248 biological processes. Through KEGG analysis, we explored the pathway of downtrend genes. A total of 183 KEGG pathways were enriched, including 17 significant pathways, such as extracellular matrix-receptor interactions. Similarly, numerous pathways related to muscle development were found, including the Wnt signaling pathway (P<0.05), MAPK signalingpathway, TGF-beta signaling pathway, and mTOR signaling pathway. Among the differentially expressed genes, we selected those involved in developing 4-time points; notably, up-regulated genes included MYH1F, SLC25A12, and HADHB, whereas the down-regulated genes included STMN1, VASH2, and TUBAL3. Conclusion: Our study explored the embryonic muscle development of the Chengkou Mountain Chicken. A large number of DEGs related to muscle development have been identified ,and validation of key genes for embryonic development and preliminary explanation of their role in muscle development. Overall, this study broadened our current understanding of the phenotypic mechanism for myofiber formation and provides valuable information for improving chicken quality.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Embryonic Muscle Development in Chengkou Mountain Chicken

Ren

Liu

Wang

et al. 2020

Preprint

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“… 29 Reverse transcription and quantitative PCR were carried out in accordance with a previous article. 30 Primers (Table 1 ) were produced by Sangon Biotech (Shanghai). Each gene was subjected to 40 cycles of PCR, and this process was repeated three times.…”

Section: Methodsmentioning

confidence: 99%

Vasorin deficiency leads to cardiac hypertrophy by targeting MYL7 in young mice

Guo

Liang

et al. 2021

J Cellular Molecular Medi

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Vasorin (VASN), also known as SLIT-like 2 (slitl2), is a 673-amino acid transmembrane glycoprotein localized on the cell surface 1 that is cleaved by a disintegrin and metalloproteinase. VASN is considered a potential biomarker and therapeutic target for cancer. 2,3 Notably, VASN is highly conserved in many organs from embryonic development to adulthood. 4,5 There is limited literature on VASN function.In embryonic development, VASN is primarily expressed in the heart and lungs. 1 VASN is expressed in vascular smooth muscle cells 1 and

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“…Especially, HDAC6 inhibition results in increased ROS production, DNA damage, apoptosis rate, and decreased blastocyst rate in early mice embryo development (H. Wang, Ling, Ai, & Bai, 2019). On the other hand, HDAC6 inhibition improves blastocyst rate in porcine IVF embryos by increasing alpha tubulin acetylation (Sun et al, 2020). In porcine and bovine embryos, activating SIRT1 increases blastocyst rate, but inhibition of SIRT1 is not effective on blastocyst rate (Adamkova et al, 2017; Idrees et al, 2022; Pollard, Gibb, Hawdon, Swegen, & Grupen, 2021).…”

Section: Hats and Hdacs In Early Embryo Developmentmentioning

confidence: 99%

Histone acetyltransferases and histone deacetyl transferases play crucial role during oogenesis and early embryo development

Bozdemir,

Uysal

2023

Genesis

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SummaryDynamic epigenetic regulation is critical for proper oogenesis and early embryo development. During oogenesis, fully grown germinal vesicle oocytes develop to mature Metaphase II oocytes which are ready for fertilization. Fertilized oocyte proliferates mitotically until blastocyst formation and the process is called early embryo development. Throughout oogenesis and early embryo development, spatio‐temporal gene expression takes place, and this dynamic gene expression is controlled with the aid of epigenetics. Epigenetic means that gene expression can be altered without changing DNA itself. Epigenome is regulated through DNA methylation and histone modifications. While DNA methylation generally ends up with repression of gene expression, histone modifications can result in expression or repression depending on type of modification, type of histone protein and its specific residue. One of the modifications is histone acetylation which generally ends up with gene expression. Histone acetylation occurs through the addition of acetyl group onto amino terminal of the core histone proteins by histone acetyltransferases (HATs). Contrarily, histone deacetylation is associated with repression of gene expression, and it is catalyzed by histone deacetylases (HDACs). This review article focuses on what is known about alterations in the expression of HATs and HDACs and emphasizes importance of HATs and HDACs during oogenesis and early embryo development.

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Improved early development potence of in vitro fertilization embryos by treatment with tubacin increasing acetylated tubulin of matured porcine oocytes

Cited by 7 publications

References 34 publications

Transcriptome Analysis of Embryonic Muscle Development in Chengkou Mountain Chicken

Transcriptome Analysis of Embryonic Muscle Development in Chengkou Mountain Chicken

Vasorin deficiency leads to cardiac hypertrophy by targeting MYL7 in young mice

Histone acetyltransferases and histone deacetyl transferases play crucial role during oogenesis and early embryo development

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