Improved drug targeting of cancer cells by utilizing actively targetable folic acid-conjugated albumin nanospheres

Shen, Zheyu; Li, Yan; Kohama, Kazuhiro; O’Neill, Brian K.; Bi, Jingxiu

doi:10.1016/j.phrs.2010.10.012

Cited by 109 publications

(68 citation statements)

References 17 publications

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“…Albumin at F has an increased viscosity and decreased dissolvability, 26 which aids its formation into nanospheres. Transmission electron microscopy ( Figure 1) showed FA-DOX-BSA MNPs to be approximately round in shape, and this has also been demonstrated by Ak et al 27 and Shen et al 28 The particles were uniform in size. Moreover, an Fe 3 O 4 area in the particle center could be seen in all particles.…”

supporting

confidence: 72%

Preparation of folic acid-conjugated, doxorubicin-loaded, magnetic bovine serum albumin nanospheres and their antitumor effects in vitro and in vivo

Yang¹,

Miao³

et al. 2014

IJN

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Background This study aimed to generate targeted folic acid-conjugated, doxorubicin-loaded, magnetic iron oxide bovine serum albumin nanospheres (FA-DOX-BSA MNPs) that lower the side effects and improve the therapeutic effect of antitumor drugs when combined with hyperthermia and targeting therapy. A new nanodrug using magnetic nanospheres for heating and addition of the folate receptor with cancer cell specificity was prepared. The characteristics of these nanospheres and their antitumor effects in nasopharyngeal carcinoma were explored. Methods FA-DOX-BSA MNPs comprising encapsulated magnetic iron oxide nanoparticles were prepared using a desolvation cross-linking method. Activated folic acid (N-hydroxysuccinimide ester of folic acid) was conjugated to the surface of albumin nanospheres via amino groups. Results Folic acid was successfully expressed on the surface of the nanospheres. Electron microscopy revealed that the FA-DOX-BSA MNPs were nearly spherical and uniform in size, with an average diameter of 180 nm. The nanomaterial could deliver doxorubicin at clinically relevant doses with an entrapment efficiency of 80%. An increasing temperature test revealed that incorporation of magnetic iron oxide into nanospheres could achieve a satisfactory heat treatment temperature at a significantly lower dose when placed in a high-frequency alternating magnetic field. FA-DOX-BSA MNPs showed greater inhibition of tumors than in the absence of folic acid in vitro and in vivo. Compared with chemotherapy alone, hyperthermia combined with chemotherapy was more effective against tumor cells. Conclusion Folic acid-conjugated bovine serum albumin nanospheres composed of mixed doxorubicin and magnetic iron oxide cores can enable controlled and targeted delivery of anticancer drugs and may offer a promising alternative to targeted doxorubicin therapy for nasopharyngeal carcinoma.

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supporting

confidence: 72%

Preparation of folic acid-conjugated, doxorubicin-loaded, magnetic bovine serum albumin nanospheres and their antitumor effects in vitro and in vivo

Yang¹,

Miao³

et al. 2014

IJN

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“…They showed that these NPs allow to the administration of the higher doses of DOX and exert remarkable anti-tumor activity in murine ascites hepatoma H22 tumor-bearing mice [35]. The similar results have been observed following the use of DOX-loaded FA-conjugated albumin nanospheres in FR-positive HeLa cells and FR-negative aortic smooth muscle cells (AoSMC) [36]. FA-targeted 5-FU-loaded BSA-carboxymethyl-β-cyclodextrin NPs have also exhibited high inhibition and promote apoptosis in FR-expressing HeLa cells as compared to free drug and non-targeted NPs [37].…”

Section: Paclitaxelsupporting

confidence: 54%

Folate-conjugated nanoparticles as a potent therapeutic approach in targeted cancer therapy

et al. 2015

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The selective and efficient drug delivery to tumor cells can remarkably improve different cancer therapeutic approaches. There are several nanoparticles (NPs) which can act as a potent drug carrier for cancer therapy. However, the specific drug delivery to cancer cells is an important issue which should be considered before designing new NPs for in vivo application. It has been shown that cancer cells over-express folate receptor (FR) in order to improve their growth. As normal cells express a significantly lower levels of FR compared to tumor cells, it seems that folate molecules can be used as potent targeting moieties in different nanocarrier-based therapeutic approaches. Moreover, there is evidence which implies folate-conjugated NPs can selectively deliver anti-tumor drugs into cancer cells both in vitro and in vivo. In this review, we will discuss about the efficiency of different folate-conjugated NPs in cancer therapy.

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“…Similar potent antitumor activity was reported Kim et al (2011) by using albumin-bound curcumin nanoparticles (CCM-HSA-NPs), and this was claimed due to the enhanced water solubility, increased accumulation in tumors and ability to traverse vascular endothelial cell. Furthermore, studies were reported (Shen et al, 2011) claiming that folic acid-conjugated albumin nanospheres had improved the targeting on HeLa cell lines. Nab-paclitaxel, an albumin bound delivery system showed superior antitumor efficacy over paclitaxel in a number of human tumor xenograft models (Desai et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

Albumin anchored docetaxel lipid nanoemulsion for improved targeting efficiency – preparation, characterization, cytotoxic, antitumor and in vivo imaging studies

et al. 2015

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The aim was to develop albumin anchored docetaxel lipid nanoemulsion (ALNE) for improving tumor targeted delivery. The O/W lipid nanoemulsion, LNEs were prepared by homogenization and ultrasonication processes. The size of globules and zeta potential were measured by Malvern Zetasizer. Albumin was coupled to stearylamine containing lipid nanoemulsion (SALNE) globules using water soluble EDC reaction. The drug content and entrapment efficiencies for the LNEs were determined by the high-performance liquid chromatography. The in vitro cytotoxic studies of the delivery systems were performed on MCF-7 and Hela cells. The IC 50 values of ALNE on both the cell lines were statistically significant. The in vivo antitumor activity was tested on solid tumors induced in C57BL/6 mice. This study revealed that the percentage tumor inhibition for the groups treated with DLNE, SALNE and ALNE when compared with untreated control was found to be 55.62 ± 5.41%, 54.27 ± 4.85% and 80.01 ± 2.74%, respectively. Furthermore, in vivo distribution studies were carried out in breast cancer MDA-MB231 xenografted Balb/c mice. The LNEs were loaded with fluorescent DiD oil and the distribution in different organs after 6 h was tracked using Caliper life sciences in vivo imaging system. The studies revealed that ALNE was superior in tumor targeting activity when compared with DLNE and SALNE by 3.04 and 2.26 folds, respectively. The average radiance values of ALNE on the tumor tissue were statistically significant when compared with DLNE, SALNE at p50.01. In addition, this strategy can become a platform technology for other lipophilic drugs to target tumors. KeywordsAlbumin tagged docetaxel lipid emulsion, antitumor study, fluorescent imaging study, improved tumor delivery, tumor targeting History

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Improved drug targeting of cancer cells by utilizing actively targetable folic acid-conjugated albumin nanospheres

Cited by 109 publications

References 17 publications

Preparation of folic acid-conjugated, doxorubicin-loaded, magnetic bovine serum albumin nanospheres and their antitumor effects in vitro and in vivo

Preparation of folic acid-conjugated, doxorubicin-loaded, magnetic bovine serum albumin nanospheres and their antitumor effects in vitro and in vivo

Folate-conjugated nanoparticles as a potent therapeutic approach in targeted cancer therapy

Albumin anchored docetaxel lipid nanoemulsion for improved targeting efficiency – preparation, characterization, cytotoxic, antitumor and in vivo imaging studies

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