Improved drug target deconvolution with PISA‐DIA using an extended, overlapping temperature gradient
Samantha J. Emery‐Corbin,
Jumana M. Yousef,
Subash Adhikari
et al.
Abstract:Thermal proteome profiling (TPP) is a powerful tool for drug target deconvolution. Recently, data‐independent acquisition mass spectrometry (DIA‐MS) approaches have demonstrated significant improvements to depth and missingness in proteome data, but traditional TPP (a.k.a. CEllular Thermal Shift Assay “CETSA”) workflows typically employ multiplexing reagents reliant on data‐dependent acquisition (DDA). Herein, we introduce a new experimental design for the Proteome Integral Solubility Alteration via label‐free… Show more
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