Improved DNA Vaccine Delivery with Needle-Free Injection Systems

Ledesma-Feliciano, Carmen; Chapman, Rosamund; Hooper, Jay W.; Elma, Kira; Zehrung, Darin; Brennan, Miles B.; Spiegel, Erin K.

doi:10.3390/vaccines11020280

Cited by 38 publications

(19 citation statements)

References 96 publications

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“…However, challenges remain with NFIS, including optimization of DNA formulations for efficient delivery, ensuring proper targeting and distribution of DNA within tissues, and minimizing potential adverse effects. PharmaJet is developing a variable-capacity NFIS, which will provide greater flexibility in delivering prophylactic vaccines.…”

Section: Delivery System For Nonviral Gene Therapymentioning

confidence: 99%

DNA-Based Nonviral Gene Therapy─Challenging but Promising

Guan,

Pei,

Song

2024

Mol. Pharmaceutics

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Over the past decades, significant progress has been made in utilizing nucleic acids, including DNA and RNA molecules, for therapeutic purposes. For DNA molecules, although various DNA delivery systems have been established, viral vector systems are the go-to choice for large-scale commercial applications. However, viral systems have certain disadvantages such as immune response, limited payload capacity, insertional mutagenesis and pre-existing immunity. In contrast, nonviral systems are less immunogenic, not size limited, safer, and easier for manufacturing compared with viral systems. What's more, nonviral DNA vectors have demonstrated their capacity to mediate specific protein expression in vivo for diverse therapeutic objectives containing a wide range of diseases such as cancer, rare diseases, neurodegenerative diseases, and infectious diseases, yielding promising therapeutic outcomes. However, exogenous plasmid DNA is prone to degrade and has poor immunogenicity in vivo. Thus, various strategies have been developed: (i) designing novel plasmids with special structures, (ii) optimizing plasmid sequences for higher expression, and (iii) developing more efficient nonviral DNA delivery systems. Based on these strategies, many interesting clinical results have been reported. This Review discusses the development of DNA-based nonviral gene therapy, including novel plasmids, nonviral delivery systems, clinical advances, and prospects. These developments hold great potential for enhancing the efficacy and safety of nonviral gene therapy and expanding its applications in the treatment of various diseases.

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Section: Delivery System For Nonviral Gene Therapymentioning

confidence: 99%

DNA-Based Nonviral Gene Therapy─Challenging but Promising

Guan,

Pei,

Song

2024

Mol. Pharmaceutics

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“…Ergo the use of ID or IM electroporation has replaced the needle delivery system. Electroporation involves application of electric current through needles, following and adjacent to the site of DNA insertion (insertion through needle and syringe) [ 64 , 65 ].…”

Section: Obstacles Encountered and Challenge To Be Fulfilled For The ...mentioning

confidence: 99%

The future of cancer immunotherapy: DNA vaccines leading the way

et al. 2023

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Immuno-oncology has revolutionized cancer treatment and has opened up new opportunities for developing vaccination methods. DNA-based cancer vaccines have emerged as a promising approach to activating the bodily immune system against cancer. Plasmid DNA immunizations have shown a favorable safety profile and there occurs induction of generalized as well as tailored immune responses in preclinical and early-phase clinical experiments. However, these vaccines have notable limitations in immunogenicity and heterogeneity and these require refinements. DNA vaccine technology has been focusing on improving vaccine efficacy and delivery, with parallel developments in nanoparticle-based delivery systems and gene-editing technologies such as CRISPR/Cas9. This approach has showcased great promise in enhancing and tailoring the immune response to vaccination. Strategies to enhance the efficacy of DNA vaccines include the selection of appropriate antigens, optimizing insertion in a plasmid, and studying combinations of vaccines with conventional strategies and targeted therapies. Combination therapies have attenuated immunosuppressive activities in the tumor microenvironment and enhanced the capability of immune cells. This review provides an overview of the current framework of DNA vaccines in oncology and focuses on novel strategies, including established combination therapies and those still under development.The challenges that oncologists, scientists, and researchers need to overcome to establish DNA vaccines as an avant-garde approach to defeating cancer, are also emphasized. The clinical implications of the immunotherapeutic approaches and the need for predictive biomarkers have also been reviewed upon. We have also tried to extend the role of Neutrophil extracellular traps (NETs) to the DNA vaccines. The clinical implications of the immunotherapeutic approaches have also been reviewed upon. Ultimately, refining and optimizing DNA vaccines will enable harnessing the immune system's natural ability to recognize and eliminate cancer cells, leading the world towards a revolution in cancer cure.

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“…Além disso, as vacinas de DNA são mais estáveis e fáceis de armazenar do que outras vacinas, o que as torna mais acessíveis em áreas remotas e de difícil acesso (38).…”

Section: Vacinas De Dnaunclassified

“…As tendências do futuro da vacinação é o desenvolvimento de vacinas mais eficazes e duradouras. A pesquisa em biotecnologia tem permitido a criação de vacinas que utilizam novas tecnologias e mecanismos de ação, como as vacinas de RNA mensageiro (mRNA) e as vacinas de vetor viral (38). Essas novas tecnologias podem permitir o desenvolvimento de vacinas mais seguras e eficazes, com maior duração da proteção contra as doenças.…”

Section: Qual O Futuro Das Doenças No Mundo?unclassified

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Análise Da Distribuição Toxicológica Do Ácido Hipúrico Na População Ocupacional Dos Postos De Combustíveis Do Centro Da Vidade De Altamira-Pará

Barros¹,

Santos²,

Oliveira³

et al. 2023

A Dinâmica Do Conhecimento Biomédico 2

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Os autores desta obra: 1. Atestam não possuir qualquer interesse comercial que constitua um conflito de interesses em relação ao artigo científico publicado; 2.Declaram que participaram ativamente da construção dos respectivos manuscritos, preferencialmente na: a) Concepção do estudo, e/ou aquisição de dados, e/ou análise e interpretação de dados; b) Elaboração do artigo ou revisão com vistas a tornar o material intelectualmente relevante; c) Aprovação final do manuscrito para submissão.; 3. Certificam que os artigos científicos publicados estão completamente isentos de dados e/ou resultados fraudulentos; 4. Confirmam a citação e a referência correta de todos os dados e de interpretações de dados de outras pesquisas; 5.Reconhecem terem informado todas as fontes de financiamento recebidas para a consecução da pesquisa; 6. Autorizam a edição da obra, que incluem os registros de ficha catalográfica, ISBN, DOI e demais indexadores, projeto visual e criação de capa, diagramação de miolo, assim como lançamento e divulgação da mesma conforme critérios da Atena Editora. DECLARAÇÃO DA EDITORA A Atena Editora declara, para os devidos fins de direito, que: 1. A presente publicação constitui apenas transferência temporária dos direitos autorais, direito sobre a publicação, inclusive não constitui responsabilidade solidária na criação dos manuscritos publicados, nos termos previstos na Lei sobre direitos autorais (Lei 9610/98), no art. 184 do Código Penal e no art. 927 do Código Civil; 2. Autoriza e incentiva os autores a assinarem contratos com repositórios institucionais, com fins exclusivos de divulgação da obra, desde que com o devido reconhecimento de autoria e edição e sem qualquer finalidade comercial; 3. Todos os e-book são open access, desta forma não os comercializa em seu site, sites parceiros, plataformas de ecommerce, ou qualquer outro meio virtual ou físico, portanto, está isenta de repasses de direitos autorais aos autores; 4. Todos os membros do conselho editorial são doutores e vinculados a instituições de ensino superior públicas, conforme recomendação da CAPES para obtenção do Qualis livro; 5. Não cede, comercializa ou autoriza a utilização dos nomes e e-mails dos autores, bem como nenhum outro dado dos mesmos, para qualquer finalidade que não o escopo da divulgação desta obra. A dinâmica do conhecimento biomédico 2 Capítulo 1

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Improved DNA Vaccine Delivery with Needle-Free Injection Systems

Cited by 38 publications

References 96 publications

DNA-Based Nonviral Gene Therapy─Challenging but Promising

DNA-Based Nonviral Gene Therapy─Challenging but Promising

The future of cancer immunotherapy: DNA vaccines leading the way

Análise Da Distribuição Toxicológica Do Ácido Hipúrico Na População Ocupacional Dos Postos De Combustíveis Do Centro Da Vidade De Altamira-Pará

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