Improved DNA extraction and purification with magnetic nanoparticles for the detection of methicillin-resistant Staphylococcus aureus

Ernst, Constanze; Bartel, Alexander; Elferink, J.W.A.; Huhn, Jennifer; Eschbach, Erik; Schönfeld, Kirsten; Feßler, Andrea T.; Oberheitmann, Boris; Schwarz, Štefan

doi:10.1016/j.vetmic.2019.01.009

Cited by 9 publications

(5 citation statements)

References 8 publications

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“…In order to detect pathogenic bacteria, many detection methods have been established. PCR is undoubtedly widely used in the detection of pathogenic bacteria, including Staphylococcus aureus [29], salmonella [30], Vibrio cholerae [31], Acinetobacter baumannii [24], Phytophthora sojae [32], and Pseudomonas aeruginosa [33]. With the continuous development of molecular diagnosis, many thermostatic amplification techniques such as LAMP, NASBA [34], and HDA [35] are gradually applied to the diagnosis of pathogenic bacteria.…”

Section: Discussionmentioning

confidence: 99%

Rapid and Sensitive Detection of Streptococcus iniae in Trachinotus ovatus Based on Multienzyme Isothermal Rapid Amplification

Wang

Niu

Sun

et al. 2023

IJMS

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Infectious diseases caused by Streptococcus iniae lead to massive death of fish, compose a serious threat to the global aquaculture industry, and constitute a risk to humans who deal with raw fish. In order to realize the early diagnosis of S. iniae, and control the outbreak and spread of disease, it is of great significance to establish fast, sensitive, and convenient detection methods for S. iniae. In the present study, two methods of real-time MIRA (multienzyme isothermal rapid amplification, MIRA) and MIRA-LFD (combining MIRA with lateral flow dipsticks (LFD)) for the simA gene of S. iniae were established, which could complete amplification at a constant temperature of 42 °C within 20 min. Real-time MIRA and MIRA-LFD assays showed high sensitivity (97 fg/μL or 7.6 × 102 CFU/mL), which were consistent with the sensitivity of real-time PCR and 10 times higher than that of PCR with strong specificity, repeatability simplicity, and rapidity for S. iniae originating from Trachinotus ovatus. In summary, real-time MIRA and MIRA-LFD provide effective ways for early diagnosis of S. iniae in aquaculture, especially for units in poor conditions.

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Section: Discussionmentioning

confidence: 99%

Rapid and Sensitive Detection of Streptococcus iniae in Trachinotus ovatus Based on Multienzyme Isothermal Rapid Amplification

Wang

Niu

Sun

et al. 2023

IJMS

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“…Genomic DNA isolation was undertaken as previously described. 26 Briefly, single colonies of MRSA isolates were cultured in BHI broth for 16 h at 37 °C. An aliquot (0.1 mL/10 8 CFU) was then centrifuged at 5 °C and the pellet resuspended in 300 mL of lysis buffer [composed of 50 mM Tris-HCl pH 8.0, 100 mM EDTA, 150 mM NaCl, 1% (v/v) SDS] supplemented with 100 mg of lysostaphin (a Staph-derived Zinc containing metalloendopeptidase) and 100 mg of RNase, before incubation for 30 min at 37 °C.…”

Section: Genomic Dna Isolation From Mrsa Isolatesmentioning

confidence: 99%

Green Synthesis of Ciprofloxacin-Loaded Cerium Oxide/Chitosan Nanocarrier and its Activity Against MRSA-Induced Mastitis

Zafar

Niazi

Menaa

et al. 2021

Journal of Pharmaceutical Sciences

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“…Further research and clinical investigations are warranted [118][119][120][121][122][123][124]. Microneedles are limited in that the application site is restricted to the arms, hands, and abdomen.…”

Section: Magnetic Nanoparticles Non-viralmentioning

confidence: 99%

“…Another potential advantage of magnetic nanoparticles is their ability to penetrate barriers such as the blood-brain barrier, allowing for non-invasive gene delivery to the central nervous system. However, further research is needed to fully understand the potential of magnetic nanoparticles in gene therapy and to optimize their delivery efficiency and safety [118][119][120][121][122][123][124].…”

Section: Magnetic Nanoparticlesmentioning

confidence: 99%

Current State of Human Gene Therapy: Approved Products and Vectors

Shchaslyvyi,

Antonenko,

Tesliuk

et al. 2023

Pharmaceuticals

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In the realm of gene therapy, a pivotal moment arrived with Paul Berg’s groundbreaking identification of the first recombinant DNA in 1972. This achievement set the stage for future breakthroughs. Conditions once considered undefeatable, like melanoma, pancreatic cancer, and a host of other ailments, are now being addressed at their root cause—the genetic level. Presently, the gene therapy landscape stands adorned with 22 approved in vivo and ex vivo products, including IMLYGIC, LUXTURNA, Zolgensma, Spinraza, Patisiran, and many more. In this comprehensive exploration, we delve into a rich assortment of 16 drugs, from siRNA, miRNA, and CRISPR/Cas9 to DNA aptamers and TRAIL/APO2L, as well as 46 carriers, from AAV, AdV, LNPs, and exosomes to naked mRNA, sonoporation, and magnetofection. The article also discusses the advantages and disadvantages of each product and vector type, as well as the current challenges faced in the practical use of gene therapy and its future potential.

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Improved DNA extraction and purification with magnetic nanoparticles for the detection of methicillin-resistant Staphylococcus aureus

Cited by 9 publications

References 8 publications

Rapid and Sensitive Detection of Streptococcus iniae in Trachinotus ovatus Based on Multienzyme Isothermal Rapid Amplification

Rapid and Sensitive Detection of Streptococcus iniae in Trachinotus ovatus Based on Multienzyme Isothermal Rapid Amplification

Green Synthesis of Ciprofloxacin-Loaded Cerium Oxide/Chitosan Nanocarrier and its Activity Against MRSA-Induced Mastitis

Current State of Human Gene Therapy: Approved Products and Vectors

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