Improved diagnosis of childhood tuberculous meningitis using more than one nucleic acid amplification test

Solomons, Regan; Visser, Douwe H.; Friedrich, Sven O.; Diacon, Andreas H.; Hoek, Kim G.P.; Marais, Ben J.; Schoeman, Johan; Furth, A. Marceline van

doi:10.5588/ijtld.14.0394

Cited by 43 publications

(39 citation statements)

References 29 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A total of 1099 articles were found in the initial search, of which 485 records were screened by title and abstract, and 20 studies were included in the final analysis [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] ( Figure 1). Table 1 of the included studies.…”

Section: Resultsmentioning

confidence: 99%

Human immunodeficiency virus in patients with tuberculous meningitis: systematic review and meta‐analysis

Pormohammad

Nasiri

Riahi

et al. 2018

Tropical Med Int Health

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Human immunodeficiency virus in patients with tuberculous meningitis: systematic review and meta‐analysis

Pormohammad

Nasiri

Riahi

et al. 2018

Tropical Med Int Health

View full text Add to dashboard Cite

show abstract

“…Another disadvantage of CSF bacterial culture is that it requires up to 72 hours for final identification. A recent study reported that CSF mycobacterial culture had a sensitivity of 22% and a specificity of 100% in diagnosis of tuberculosis meningitis 29 . For viruses, the application of monoclonal antibodies in shell vial culture has increased the speed and specificity.…”

Section: Conventional Microbiology Methodsmentioning

confidence: 99%

Laboratory Diagnosis of Central Nervous System Infection

Kaplan

Kamboj

et al. 2016

Curr Infect Dis Rep

View full text Add to dashboard Cite

CNS infections are potentially life threatening if not diagnosed and treated early. The initial clinical presentations of many CNS infections are nonspecific making a definitive etiologic diagnosis challenging. Nucleic acid in vitro amplification-based molecular methods are increasingly being applied for routine microbial detection. These methods are a vast improvement over conventional techniques with the advantage of rapid turnaround and higher sensitivity and specificity. Additionally, molecular methods performed on cerebrospinal fluid samples are considered the new gold standard for diagnosis of CNS infection caused by pathogens which are otherwise difficult to detect. Commercial diagnostic platforms offer various monoplex and multiplex PCR assays for convenient testing of targets that cause similar clinical illness. Pan-omic molecular platforms possess potential for use in this area. Although molecular methods are predicted to be widely used in diagnosing and monitoring CNS infections, results generated by these methods need to be carefully interpreted in combination with clinical findings. This review summarizes the currently available armamentarium of molecular assays for diagnosis of central nervous system infections, their application, and future approaches.

show abstract

“…The sensitivity of these tests was 73.1% for GenoType MTBDRplus and 71.2% for Xpert MTB/RIF in culture-positive patients, and of 57 and 58%, respectively, in smear-negative cases [91]. A study done exclusively in children evaluated GenoType MTBDRplus performance in TB meningitis, using TB meningitis case definition as reference for diagnosis [92]. GenoType MTBDRplus sensitivity and specificity were 33 and 98%, respectively.…”

Section: Genotype Mtbdrplusmentioning

confidence: 99%

“…GenoType MTBDRplus sensitivity and specificity were 33 and 98%, respectively. Moreover, combining culture, GenoType MTBDRplus and Xpert MTB/RIF, the sensitivity raised to 56% [92]. The main limitations of GenoType MTBDRplus are the need of appropriate laboratory facilities and expert technical abilities, resulting in more complex than Xpert MTB/RIF, especially in low-resource settings.…”

Section: Genotype Mtbdrplusmentioning

confidence: 99%

What steps do we need to take to improve diagnosis of tuberculosis in children?

Venturini

Remaschi

Berti

et al. 2015

Expert Review of Anti-infective Therapy

View full text Add to dashboard Cite

Tuberculosis still represents a big global public health challenge. The diagnosis of tuberculosis and the differentiation between active and latent tuberculosis remain difficult, particularly in childhood, because of the lack of a gold standard test for diagnosis. In the last decade, novel diagnostic assays have been developed. Among immunologic tests, new assays based on the measurement of different cytokines released by specific T cells in response to Mycobacterium tuberculosis antigens, other than INF-γ, have been investigated. Promising results rely on nucleic acid amplification techniques, also able to detect drugs resistance. Innovative research fields studied the modifications of CD27 expression in T cells as well as different host gene expression in response to M. tuberculosis. Further studies are needed to assess the diagnostic value and the accuracy of these new assays.

show abstract

Improved diagnosis of childhood tuberculous meningitis using more than one nucleic acid amplification test

Abstract: Although commercial nucleic-acid amplification tests performed on CSF revealed incrementally improved diagnostic accuracy, providing rapid microbiological confirmation, they cannot serve as a rule-out test.

Cited by 43 publications

References 29 publications

Human immunodeficiency virus in patients with tuberculous meningitis: systematic review and meta‐analysis

Human immunodeficiency virus in patients with tuberculous meningitis: systematic review and meta‐analysis

Laboratory Diagnosis of Central Nervous System Infection

What steps do we need to take to improve diagnosis of tuberculosis in children?

Contact Info

Product

Resources

About