Improved determination of phytate by ion-exchange chromatography

Bos, K. D.; Verbeek, Cornelis; Eeden, C. H. Peter Van; Slump, P.; Wolters, M. G. E.

doi:10.1021/jf00010a015

Cited by 48 publications

(19 citation statements)

References 4 publications

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“…(c) Techniques for the separation of inositol phosphates (i) Liquid chromatography Several methods have been used to separate inositol phosphates, including ion-exchange (high performance liquid chromatography (HPLC) or conventional largecolumn ion-exchange) and size exclusion/gel filtration (table 3), although most have involved ion-exchange chromatography to isolate the individual congeners (Tangendjaja et al 1980;Camire & Clydesdale 1982;Graf & Dintzis 1982;Batty et al 1985;Wilson et al 1985;Cilliers & Van Niekerk 1986;Meek & Nicoletti 1986;Portilla & Morrison 1986;Horstman et al 1988;Matthews et al 1988;Mayr 1988;Minear et al 1988;Phillipy & Bland 1988;Heathers et al 1989;Taylor et al 1990;Bos et al 1991;Prestwich & Bolton 1991;Clarkin et al 1992;Rounds & Nielsen 1993;Wolters et al 1993;Nanny et al 1994Nanny et al , 1995Guse 1995;Suzumura & Kamatani 1995b). The ability of anion-exchange HPLC to separate the various inositol phosphate congeners effectively and efficiently has led to its widespread use.…”

Section: Analytical Techniques For the Determination Of Inositol Phosmentioning

confidence: 99%

Inositol phosphates in the environment

Turner¹,

Paphazy

Haygarth³

et al. 2002

Phil. Trans. R. Soc. Lond. B

673

554

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The inositol phosphates are a group of organic phosphorus compounds found widely in the natural environment, but that represent the greatest gap in our understanding of the global phosphorus cycle. They exist as inositols in various states of phosphorylation (bound to between one and six phosphate groups) and isomeric forms (e.g. myo, d-chiro, scyllo, neo), although myo-inositol hexakisphosphate is by far the most prevalent form in nature. In terrestrial environments, inositol phosphates are principally derived from plants and accumulate in soils to become the dominant class of organic phosphorus compounds. Inositol phosphates are also present in large amounts in aquatic environments, where they may contribute to eutrophication. Despite the prevalence of inositol phosphates in the environment, their cycling, mobility and bioavailability are poorly understood. This is largely related to analytical difficulties associated with the extraction, separation and detection of inositol phosphates in environmental samples. This review summarizes the current knowledge of inositol phosphates in the environment and the analytical techniques currently available for their detection in environmental samples. Recent advances in technology, such as the development of suitable chromatographic and capillary electrophoresis separation techniques, should help to elucidate some of the more pertinent questions regarding inositol phosphates in the natural environment.

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Section: Analytical Techniques For the Determination Of Inositol Phosmentioning

confidence: 99%

Inositol phosphates in the environment

Turner¹,

Paphazy

Haygarth³

et al. 2002

Phil. Trans. R. Soc. Lond. B

673

554

View full text Add to dashboard Cite

show abstract

“…Phytic acid determination in solid samples can be conducted in various ways, such as extracting, precipitating, and purifying the precipitate and measuring it directly or indirectly using an anion exchange column (AEC) method, high-performance liquid chromatography (HPLC), or colorimetric method. In 1971, Oberleas used the precipitation of phytate-iron complex to analyze phytic acid; however, this method had difficulty with low phytate concentration in food (Bos et al 1991). Oberleas and Harland, in 1977, improved the Oberleas method using an AEC method (Bos et al 1991).…”

Section: Introductionmentioning

confidence: 99%

“…In 1971, Oberleas used the precipitation of phytate-iron complex to analyze phytic acid; however, this method had difficulty with low phytate concentration in food (Bos et al 1991). Oberleas and Harland, in 1977, improved the Oberleas method using an AEC method (Bos et al 1991). In this method, the phytic acid was extracted from the food using a feed matrix with 2.4 % of HCl solution.…”

Section: Introductionmentioning

confidence: 99%

“…In 1983, this method was further improved by Ellis and Morris who extracted the phytate from the anion exchange column using EDTA, which helped to eliminate metal ion interferences. The AEC method was laborious and only able to achieve good results if samples were processed in batches (Bos et al 1991). In 1986, Clilliers and van Niekerk introduced the HPLC method and conducted the AEC procedures before extracting the phytic acid with EDTA (Bos et al 1991).…”

Section: Introductionmentioning

confidence: 99%

“…The AEC method was laborious and only able to achieve good results if samples were processed in batches (Bos et al 1991). In 1986, Clilliers and van Niekerk introduced the HPLC method and conducted the AEC procedures before extracting the phytic acid with EDTA (Bos et al 1991). However, HPLC equipment is rather expensive, and the method is time consuming (McMaster 2007).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Determination of Phytic Acid in Juices and Milks by Developing a Quick Complexometric-Titration Method

Burgos-Luján

Tong

2014

Food Anal. Methods

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Phytic acid is a compound regularly found in plantbased food and beverages. Although it may provide some health benefits to human body, it is commonly defined as an antinutrient that reduces the availability of nutrients. Analytical methods were reported in literature for measuring phytic acid in cereals, which cannot be applied to liquid samples directly. A simple and quick complexometrictitration method specialized for determination of phytic acid in liquid samples was developed. Phytic acid was indirectly measured through a complex formation titration with ferric ions and ethylenediaminetetraacetic acid (EDTA). The new analytical method had a detection limit of 12 mg/L and was applied to various beverages including juices and milks. Phytic acids were found in all samples obtained in a local grocery store. Juices showed higher phytic acid content than milks. At 5.35 g/L, soy sauce had the highest level of phytic acid. Although the lowest concentration was detected in cow milk at 328 mg/L, phytic acid may still be a concern due to high-volume consumption of milk.

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Influence of different types of protein on in vitro availability of intrinsic and extrinsic iron and zinc

Pérez‐Llamas

Diepenmaat-Wolters

Zamora

1997

J. Sci. Food Agric.

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: The inÑuence of beef, pork and soy proteins on intrinsic and extrinsic iron and zinc in vitro availability was investigated, by measuring mineral dialysability. Intrinsic iron dialysability (percentage of iron dialysed) was similar in the animal proteins and higher than that from soy protein isolate (P \ 0É001). Intrinsic zinc dialysability from pork protein was statistically (P \ 0É001) higher than that from other proteins studied. Beef and soy proteins do not di †er in their percentages of zinc availability. The e †ect on the extrinsic iron availability was di †erent among the three proteins studied, but not on the extrinsic zinc availability. The composition of amino acids and/or the small peptides produced during the digestion process of the protein can modify the iron, and probably the zinc, availability. The inorganic zinc addition (10 kg ml~1) reduced the nonhaem and extrinsic iron availability, while it did not a †ect the haem iron availability. At the concentrations used in this study (10 kg ml~1) a decrease of zinc dialysability caused by the inorganic iron addition was not observed.

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Improved determination of phytate by ion-exchange chromatography

Cited by 48 publications

References 4 publications

Inositol phosphates in the environment

Inositol phosphates in the environment

Determination of Phytic Acid in Juices and Milks by Developing a Quick Complexometric-Titration Method

Influence of different types of protein on in vitro availability of intrinsic and extrinsic iron and zinc

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