Improved Depth-of-Field Photoacoustic Microscopy with a Multifocal Point Transducer for Biomedical Imaging

Nguyen, Thanh Phuoc; Nguyen, Van Tu; Pham, Van Hiep; Vu, Dinh Dat; Kim, Byung-Gak; Oh, Junghwan

doi:10.3390/s20072020

Cited by 13 publications

(7 citation statements)

References 33 publications

(44 reference statements)

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“…Maximum visualization depth is limited by transducer's frequency, gain, TGC (Time Gain Compensation), focus depth, display format (number of scan lines), output power, and noise of the electronic system. Depth of penetration analysis is performed by scanning the phantom and freezing (choosing) the image, making measurement, and recording visualization of maximum depth of default echo, distance from scanning window to the deepest cylinder or the round object that is barely visible [9,10].…”

Section: Methodsmentioning

confidence: 99%

Carrageenan Biopolymer for Medical Ultrasonography using Phantom CIRS

Diartama¹,

Suryono

Sugiyanto

et al. 2020

E3S Web Conf.

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The use of polymer as conductor in medical ultrasonography is very crucial to establish patient diagnosis and to prevent administration of improper treatment. Rapid development in science and technology encourages people to create more innovations that are readily usable. However, these innovations sometimes put health and environmental aspects aside that in turn become disadvantageous to health and may lead to environmental pollution. This research proposes the use of carrageenan biopolymer as a safe alternative for people’s health and the environment. It aims to prove differences in resulting phantom CIRS images taken with the help of carrageenan gel and standard gel based on Carbomer 940 as acoustic coupling agents (ACA) for ultrasonography (USG). It is an experimental research that uses true experiment technique with posttest only control group design. Results from T-test analysis show significance value of p>0.05, meaning there is no significant difference between the use of carrageenan gel and ACA gel (Carbomer 940). This result proves that carrageenan gel can be used as an acoustic coupling agent for ultrasonography. Results from imaging tests also reveal that there is no difference between the use of carrageenan gel and that of a manufacturer gel based on Carbomer 940 available in Indonesia, in terms of visualization of phantom CIRS images.

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Section: Methodsmentioning

confidence: 99%

Carrageenan Biopolymer for Medical Ultrasonography using Phantom CIRS

Diartama¹,

Suryono

Sugiyanto

et al. 2020

E3S Web Conf.

View full text Add to dashboard Cite

show abstract

“…3 mm depth without compromising resolution. 24 With standard objectives, 2PLSM is restricted only to imaging near-surface areas (up to 1 mm depth) due to the relatively inefficient collection of emission light. It is therefore necessary to design and produce implantable probes that reliably extend the range of imaging techniques while causing minimal damage to the surrounding tissue.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Photoacoustic microscopy is limited to approx. 3 mm depth without compromising resolution . With standard objectives, 2PLSM is restricted only to imaging near-surface areas (up to 1 mm depth) due to the relatively inefficient collection of emission light.…”

Section: Introductionmentioning

confidence: 99%

Novel Design and Application of High-NA Fiber Imaging Bundles for In Vivo Brain Imaging with Two-Photon Scanning Fluorescence Microscopy

Bijoch

Włodkowska

Kasztelanic

et al. 2023

ACS Appl. Mater. Interfaces

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Here, we provide experimental verification supporting the use of short-section imaging bundles for two-photon microscopy imaging of the mouse brain. The 8 mm long bundle is made of a pair of heavymetal oxide glasses with a refractive index contrast of 0.38 to ensure a high numerical aperture NA = 1.15. The bundle is composed of 825 multimode cores, ordered in a hexagonal lattice with a pixel size of 14 μm and a total diameter of 914 μm. We demonstrate successful imaging through custom-made bundles with 14 μm resolution. As the input, we used a 910 nm Ti-sapphire laser with 140 fs pulse and a peak power of 9 × 10 4 W. The excitation beam and fluorescent image were transferred through the fiber imaging bundle. As test samples, we used 1 μm green fluorescent latex beads, ex vivo hippocampal neurons expressing green fluorescent protein and cortical neurons in vivo expressing the fluorescent reporter GCaMP6s or immediate early gene Fos fluorescent reporter. This system can be used for minimal-invasive in vivo imaging of the cerebral cortex, hippocampus, or deep brain areas as a part of a tabletop system or an implantable setup. It is a low-cost solution, easy to integrate and operate for high-throughput experiments.

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“…Ultrasonic transducers are important, well-known devices related to image acquisition in biomedical applications [ 1 , 2 , 3 ], corrosion inspection, and defecting surface breaking cracks in industry [ 4 , 5 ]. These devices are made from several piezoelectric materials, depending on their applications.…”

Section: Introductionmentioning

confidence: 99%

Design and Micro-Fabrication of Focused High-Frequency Needle Transducers for Medical Imaging

Nguyen¹,

Choi

Nguyen

et al. 2022

Sensors

Self Cite

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In this study, we report an advanced fabrication technique to develop a miniature focused needle transducer. Two different types of high-frequency (100 MHz) transducers were fabricated using the lead magnesium niobate-lead titanate (PMN-0.3PT) and lithium niobate (LiNbO3) single crystals. In order to enhance the transducer’s performance, a unique mass–spring matching layer technique was adopted, in which gold and parylene play the roles of the mass layer and spring layer, respectively. The PMN-0.3PT transducer had a 103 MHz center frequency with a −6 dB bandwidth of 52%, and a signal-to-noise ratio (SNR) of 42 dB. The center frequency, −6 dB bandwidth, and SNR of the LiNbO3 transducer were 105 MHz, 66%, and 44 dB, respectively. In order to compare and evaluate the transducers’ performances, an ultrasonic biomicroscopy (UBM) imaging on the fish eye was performed. The results showed that the LiNbO3 transducer had a better contrast resolution compared to the PMN-0.3PT transducer. The fabricated transducer showed an excellent performance with high-resolution corneal epithelium imaging of the experimental fish eye. These interesting findings are useful for the future biomedical implementation of the fabricated transducers in the field of high-resolution ultrasound imaging and diagnosis purpose.

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Improved Depth-of-Field Photoacoustic Microscopy with a Multifocal Point Transducer for Biomedical Imaging

Cited by 13 publications

References 33 publications

Carrageenan Biopolymer for Medical Ultrasonography using Phantom CIRS

Carrageenan Biopolymer for Medical Ultrasonography using Phantom CIRS

Novel Design and Application of High-NA Fiber Imaging Bundles for In Vivo Brain Imaging with Two-Photon Scanning Fluorescence Microscopy

Design and Micro-Fabrication of Focused High-Frequency Needle Transducers for Medical Imaging

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