Improved cytocompatibility and antibacterial properties of zinc-substituted brushite bone cement based on β-tricalcium phosphate

Abstract

For bone replacement materials, osteoconductive, osteoinductive, and osteogenic properties are desired. The bacterial resistance and the need for new antibacterial strategies stand among the most challenging tasks of the modern medicine. In this work, brushite cements based on powders of Zinc (Zn) (1.4 wt%) substituted tricalcium phosphate (β-TCP) and non-substituted β-TCP were prepared and investigated. Their initial and final phase composition, time of setting, morphology, pH evolution, and compressive strength are reported. After soaking for 60 days in physiological solution, the cements transformed into a mixture of brushite and hydroxyapatite. Antibacterial activity of the cements against Enterococcus faecium, Escherichia coli, and Pseudomonas aeruginosa bacteria strains was attested. The absence of cytotoxicity of cements was proved for murine fibroblast NCTC L929 cells. Moreover, the cell viability on the β-TCP cement containing Zn2+ ions was 10% higher compared to the β-TCP cement without zinc. The developed cements are perspective for applications in orthopedics and traumatology.

Exploring the Journey of Zinc Oxide Nanoparticles (ZnO-NPs) toward Biomedical Applications

“…Additionally, these NPs are employed in the cosmetics industry to produced sunblock creams, which guard the human body against ultraviolet radiations [ 4 ]. Due to ZnO-NPs’ characteristics, such as their biocompatibility and non-toxicity, they are particularly well-suited for specialized biomedical applications [ 5 , 6 , 7 ]. Metal oxide NPs are important components in a wide range of consumer goods, including electronic equipment and cosmetics.…”

Influence of Synthesis Conditions on Gadolinium-Substituted Tricalcium Phosphate Ceramics and Its Physicochemical, Biological, and Antibacterial Properties

“…It is possible to regulate the rate of resorption, physical features, and biological performance by cationic or anionic substitutions in the β -TCP structure [ 9 , 10 , 11 ]. For example, doping the β -TCP structure with various metal cations, such as copper (II), manganese (II), iron (III), and zinc (II) [ 12 , 13 , 14 , 15 ], leads to its destabilization and, consequently, to the increase in the rate of dissolution of these cations in the body fluid. In addition to the modulation of physicochemical characteristics of β -TCP, metal-substituted TCPs provide new advanced properties to the materials.…”

“…In addition to the modulation of physicochemical characteristics of β -TCP, metal-substituted TCPs provide new advanced properties to the materials. Antibacterial characteristics can be imparted by Cu 2+ , Fe 3+ , Zn 2+ , and Ag + ions [ 12 , 14 , 15 , 16 ], and the biocompatibility properties and, namely, cell proliferation on the materials surface can be improved by Fe 3+ and Sr 2+ ions substitution [ 5 , 14 , 17 , 18 , 19 ]. Mg-doped β -TCP biomaterials are able to enhance the cell proliferation and viability of human bone marrow-derived mesenchymal stem cells [ 20 ] and the differentiation of hBMSCs into osteoblasts [ 21 ].…”

Bioactive Properties of Composites Based on Silicate Glasses and Different Silver and Gold Structures

“…Unfortunately, surgeries sometime end with a bacterial infection that is received during hospital treatment [ 13 ]. Therefore, it is important to develop materials that exhibit antibacterial properties by involving noble metals in them, such as Ag, Pt, Pd, Cu, and Ru [ 12 , 14 ], or metals such as Ti, Co, Zn, and Ni [ 15 , 16 ]. Bioactive glass with Ag nanoparticles (AgNPs) showed high potential in antibacterial applications, as claimed by Bellantone and Hench [ 17 ].…”