Improved cross-axis synchronous flow-through coil planet centrifuge for performing counter-current chromatography

Countercurrent Separation of Natural Products

“…9 Separation of gramicidins with CHCl 3benzene-MeOH-water was performed in 1982 71 and of bacitracin with CHCl 3 -95% EtOH-water in 1989. 131 The combination of CHCl 3 -MeOH-water was first reported in 1985. Siderochelin A and pentalenolactone were isolated using CHCl 3 -MeOH-water (7:13:8 and 1:1:1, respectively) by Brill et al 132 This combination proved to be very useful and was used in many subsequent separations throughout the 1980s and 1990s.…”

“…In fact, the first documented use of DCCC was in the separation of a DNP−amino acid mixture with CHCl 3 −acetic acid−0.1 N aqueous HCl (2:2:1) . Separation of gramicidins with CHCl 3 −benzene−MeOH−water was performed in 1982 and of bacitracin with CHCl 3 −95% EtOH−water in 1989 . The combination of CHCl 3 −MeOH−water was first reported in 1985.…”

Purification of Proteins From Cell-Culture Medium or Cell-Lysate by High-Speed Counter-Current Chromatography Using Cross-Axis Coil Planet Centrifuge

“…The detailed design of the cross-axis CPC was described elsewhere [37,38]. The apparatus holds a pair of horizontal rotary shafts, symmetrically mounted one on each side of the rotary frame, at a distance of 10 cm from the centrifuge axis as shown in Fig.…”

Purification of alcohol dehydrogenase from bovine liver crude extract by dye–ligand affinity counter-current chromatography

“…able retention of the stationary phase for viscous polymer phase systems. The detailed design of the x-axis CPC was described elsewhere [9,10]. The present apparatus holds a pair of column holders in the lateral position at 10 cm from the center of the rotary shaft horizontally mounted on the rotary frame at 10 cm from the central axis of the apparatus as shown in Fig.…”