Improved conidiation from entomopathogenic fungi through 26% oxygen pulses in solid‐state culture depends on a balance between headspace volume and substrate amounts

Abstract

Oxygen‐enriched atmospheres applied as periodic pulses increased conidia production from entomopathogenic fungi in agar surface cultures. However, this advantage has not been obtained in solid‐state cultures (SSC), probably as a result of different biomass production between both culture systems. In this work, the biomass formation from two Isaria strains was limited in SSC using 5, 2·5 and 1 initial grams of substrate (gds). In the system with 5 gds, conidia production decreased in 26% oxygen‐enriched pulses compared to the normal atmosphere. Conversely, 26% oxygen pulses increased conidiation up to one order magnitude in systems with 2·5 and 1 gds, respective to the normal atmosphere. These results were explained by oxygen depletion and high CO2 accumulation in the 5 gds system. Whereas in systems with 2·5 or 1 gds, oxygen levels remained high enough to stimulate conidiation. These results were attributed to the headspace volume:gds ratio, which is suggested to be ≥48 ml per gds. This ratio is proposed as a scaling‐up criterion for bioreactor design when oxygen‐enriched pulses are used in SSC for improvement of conidia production. Significance and Impact of the Study Oxygen‐enriched atmospheres applied as periodic pulses increase conidiation in entomopathogenic fungi (EF). However, this remained restricted to agar surface cultures, since conidiation decreased when carried out in solid‐state culture (SSC) which is used as large‐scale production system. We identified that in SSC the ratio between the headspace volume containing 26% oxygen‐enriched pulses and the grams of substrate determines the conidiation response to oxygen‐enriched pulses. For the first time, oxygen‐enriched pulses increased conidiation in SSC respective to the normal atmosphere in four EF. This ratio is proposed as a bioreactor criterion design for large‐scale conidia production of EF using oxygen‐enriched pulses.