Improved Colorimetric Determination of Cell Wall Chitin in Wood Decay Fungi

Chen, George C.; Johnson, Bruce R.

doi:10.1128/aem.46.1.13-16.1983

Cited by 111 publications

(27 citation statements)

References 21 publications

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“…trimethylsilyl derivatives by GLC-MS. Infrared (IR) spectra are obtained using the KBr technique (Price, 1972). Aminosugar content is determined after hydrolysis of the polysaccharides according to Chen & Johnson (1983), and these compounds are identified in an amino acid analyser. The absolute configuration of the monosaccharides is determined after hydrolysis of the polysaccharides and derivatization according to Gerwig et al (1979) by GLC-MS of the tetra-O-TMSi-(1)-2-butylglycosides obtained.…”

Section: Characterizationmentioning

confidence: 99%

An assessment of fungal wall heteromannans as a phylogenetically informative character in ascomycetes

et al. 2010

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The fungal wall contains a small proportion of alkali-extractable water-soluble heteromannans (F1SS). They are the glycidic moiety of glycoproteins that have important roles in the biology of fungi. A considerable number of these polysaccharides has been described, differing in composition or linkage types. Their structure is similar in all species of a well-delimited genus, and teleomorphs and their corresponding anamorphs. Therefore, these polysaccharides have been used as chemotaxonomic markers at the genus level. Here we review cases where they have been found to resolve relationships around the genus level, and assess their phylogenetic informativeness in the delineation of taxa at family and higher ranks in the ascomycetes by comparison with molecular trees. Generally, the correlation is extremely good, from the species to the class level, though there are some divergences. In particular, comparisons suggest that the concept of the Sordariomycetes may eventually require revision as more molecular data become available. An analysis of the different chemical structures of these polysaccharides can lead to the proposal and testing of phylogenetic hypotheses, in a parallel manner to those generated from molecular trees. These molecules serve as an independent character similar to morphological or molecular characters.

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Section: Characterizationmentioning

confidence: 99%

An assessment of fungal wall heteromannans as a phylogenetically informative character in ascomycetes

et al. 2010

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“…Total neutral polysaccharides were analyzed by anthrone reagent with glucose as standard (Fairbairn 1953), and chitin was measured colorimetrically with 3-methyl-2-benzothiazolone hydrazone by the Ride and Drysdale (1972) method. Total and extracted chitin in AIS was determined after acid hydrolysis as glucosamine (Chen and Johnson 1983).…”

Section: Physiochemical Analysesmentioning

confidence: 99%

Mushroom Texture, Cell Wall Composition, Color, and Ultrastructure as Affected by pH and Temperature

Zivanovic¹,

Buescher²,

Kim³

2003

Journal of Food Science

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Pasteurized mushrooms had firmer texture and lighter color compared with those that were retorted. Addition of acid and retorting temperature resulted in extensive softening of mushrooms and solubilization of proteins and polysaccharides that diffused into brine. Total chitin was not affected by applied treatments and did not appear to be related to texture alteration of processed mushrooms. Extensive solubilization caused by acidification and retortion resulted in reduction of cell diameter and enlarged intercellular space within the cap tissue. Mild acidification of sterilized samples reduced discoloration compared with the control, but further acidification resulted in considerable darkening. In contrast, tissue and color of pasteurized mushrooms seemed to be unaffected.

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“…In AIS and in all fractions, total neutral carbohydrates were analyzed by anthrone reagent with glucose as standard (Fairbairn 1953), and chitin was measured by the Ride and Drysdale (1972) method. Total chitin in AIS was determined after acid hydrolysis (Chen and Johnson 1983). Monosaccharides in hydrolyzed alcohol insoluble solids were analyzed by GC as alditol acetates (Blakeney and others 1983).…”

Section: Preparation Sequential Extraction and Analysis Of Alcohol mentioning

confidence: 99%

Textural Changes in Mushrooms (Agaricus bisporus) Associated with Tissue Ultrastructure and Composition

Zivanovic¹,

Busher²,

Kim³

2000

Journal of Food Science

126

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The objective of this study was to determine ultrastructural and compositional changes in fresh mushrooms associated with adverse changes in texture during 9 d of post-harvest storage at 12°C. Mushroom softening (2.6N to 1.5N, puncturing force) was consistent with toughening (19.2N to 33.0N, gumminess). Protein and total carbohydrate content declined, but chitin content increased during mushroom storage. Most polysaccharides were extracted after deacetylation and depolymerization of chitin, indicating that structural glucans were mainly bound with acetylglucosamine polymers. Softening paralleled expansion of intercellular space at the pilei surface, hyphae shrinkage, central vacuole disruption, and loss of proteins (r = 0.94) and polysaccharides (r = 0.84), while toughening was associated with increased chitin content (r = 0.95).

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Improved Colorimetric Determination of Cell Wall Chitin in Wood Decay Fungi

Cited by 111 publications

References 21 publications

An assessment of fungal wall heteromannans as a phylogenetically informative character in ascomycetes

An assessment of fungal wall heteromannans as a phylogenetically informative character in ascomycetes

Mushroom Texture, Cell Wall Composition, Color, and Ultrastructure as Affected by pH and Temperature

Textural Changes in Mushrooms (Agaricus bisporus) Associated with Tissue Ultrastructure and Composition

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