Improved collagen type I/III ratio at the interface of gentamicin-supplemented polyvinylidenfluoride mesh materials

Junge, K.; Klinge, U.; Rösch, R.; Lynen, Petra; Binnebösel, Marcel; Conze, Joachim; Mertens, Peter R.; Schwab, Robert; Schumpelick, Volker

doi:10.1007/s00423-006-0138-1

Cited by 48 publications

(43 citation statements)

References 29 publications

(31 reference statements)

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“…These results confirm the finding of previous studies with gentamicin-coated PVDF mesh material, which initially was constructed for infection prophylaxis in hernia repair [24,25].…”

Section: Discussionsupporting

confidence: 94%

“…Due to the RNAbinding capacity of gentamicin, it directly influences the collagen metabolism by inhibition of prolidase, an enzyme involved in collagen synthesis and collagen degradation [23]. Furthermore, Junge et al described an increased ratio of mature, highly cross-linked collagen type I to premature less cross-linked collagen type III at the interface of gentamicin-supplemented PVDF mesh materials [24,25].…”

Section: Introductionmentioning

confidence: 98%

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Impact of gentamicin-supplemented polyvinylidenfluoride mesh materials on MMP-2 expression and tissue integration in a transgenic mice model

Binnebösel

Ricken

Klink

et al. 2010

Langenbecks Arch Surg

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“…These results confirm the finding of previous studies with gentamicin-coated PVDF mesh material, which initially was constructed for infection prophylaxis in hernia repair [24,25].…”

Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 98%

Impact of gentamicin-supplemented polyvinylidenfluoride mesh materials on MMP-2 expression and tissue integration in a transgenic mice model

Binnebösel

Ricken

Klink

et al. 2010

Langenbecks Arch Surg

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“…With regard to a desired modulation of collagen quality just gentamicin has been shown to be effective [188]. Using the described gentamicin surface modificated PVDF mesh we investigated the modulation of extracellular matrix, and next to the known antibiotic effect, the quality of the surrounding scar formation was improved.…”

Section: Extracellular Matrix Modulationmentioning

confidence: 99%

Mesh biocompatibility: effects of cellular inflammation and tissue remodelling

Junge

Binnebösel

Trotha

et al. 2011

Langenbecks Arch Surg

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122

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Mesh biocompatibility is basically determined by the foreign body reaction (FBR). In contrast to physiological wound healing and scar formation, the FBR at the host-tissue/biomaterial interface is present for the lifetime of the medical device. The cellular interactions at the mesh/tissue interface proceed over time ending up in a chronic inflammatory process. The time course of the FBR has been studied extensively and consists of three crucial steps that are protein absorption, cell recruitment and, finally, fibrotic encapsulation and extracellular matrix formation. Each of these steps involves a complex cascade of immune modulators including soluble mediators and various cell types. Recent research has focused on the cellular and molecular interactions of the distinct phases of the FBR offering a new basis for therapeutical strategies. The highly dynamic process of the FBR is considerably influenced by the biomaterial composition. Modifications of the type of polymer, the material weight, the filament structure and the pore size are realized and have substantial effects on the in vivo biocompatibility. Moreover, modern mesh technology aims to utilize the available implants as carrier systems for bioactive drugs. Studies in animal models account for the efficiency of these drugs that aim to reduce mesh-related infections or to minimize FBR by influencing inflammation or extracellular matrix remodelling. A thorough understanding of the molecular mechanisms of FBR provides a sophisticated background for the development of new biomaterials at least as carrier systems for bioactive reagents to reduce inflammation and to improve clinical outcome.

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“…The vast majority of existing experimental IH models have focused on creating regular fascia defects and subsequently applying treatment modalities such as meshes or biologic growth factors [11,12,13,14,15,16,17]. Therefore, these existing models are less suitable for investigating the changes in the early fascia healing after abdominal incisions that potentially may lead to IH formation.…”

Section: Introductionmentioning

confidence: 99%

An Experimental Animal Model for Abdominal Fascia Healing after Surgery

Burcharth

Pommergaard²,

Klein³

et al. 2013

Eur Surg Res

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Background: Incisional hernia (IH) is a well-known complication after abdominal surgical procedures. The exact etiology of IH is still unknown even though many risk factors have been suggested. The aim of this study was to create an animal model of a weakly healed abdominal fascia that could be used to evaluate the actively healing fascia. Such an animal model may promote future research in the prevention of IH. Methods: 86 male Sprague-Dawley rats were used to establish a model involving six experiments (experiments A-F). Mechanical testing of the breaking strength of the healed fascia was performed by testing tissue strips from the healed fascia versus the unincised control fascia 7 and 28 days postoperatively. Results: During the six experiments a healing model was created that produced significantly weaker coherent fascia when compared with the control tissue measured in terms of mechanical breaking strength. This new animal model employed myofascial closing after a full thickness abdominal incision using a running suture with fast-absorbable suture material. This technique produced a weak myofascial layer compared with the control tissue measured in terms of breaking strength after both 7 and 28 days. Conclusion: It was possible to create a healed but weak abdominal fascia in rats with a minimum of defects after incision using a running suture technique in the fascia. In contrast to other models, regular tissue defects were absent, and the model can therefore be used to evaluate the changes, both histological and mechanical, in the actively healing fascia, which may lead to incisional herniation. Furthermore, the model may also be used to study interventions performed to promote healing. This contrasts with existing models with regular fascia defects, which are not well suited for this purpose.

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Improved collagen type I/III ratio at the interface of gentamicin-supplemented polyvinylidenfluoride mesh materials

Abstract: The present data confirm that a surface modification of PVDF mesh samples using plasma-induced graft polymerization of acrylic acid and supplementation of gentamicin is able to improve scar quality and mesh integration.

Cited by 48 publications

References 29 publications

Impact of gentamicin-supplemented polyvinylidenfluoride mesh materials on MMP-2 expression and tissue integration in a transgenic mice model

Impact of gentamicin-supplemented polyvinylidenfluoride mesh materials on MMP-2 expression and tissue integration in a transgenic mice model

Mesh biocompatibility: effects of cellular inflammation and tissue remodelling

An Experimental Animal Model for Abdominal Fascia Healing after Surgery

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