Improved cellular uptake of chitosan-modified PLGA nanospheres by A549 cells

The therapeutic effect of methotrexate-conjugated Pluronic-based polymeric micelles on the folate receptor-rich tumors treatment

“…2 In our previous studies, polymeric micelles prepared with Pluronic F127 and P105 have been demonstrated to enhance anticancer efficacy of docetaxel and methotrexate (MTX) against multidrug-resistant (MDR) cancer cells in vitro and in vivo. [3][4][5] Pluronic P105 is presented as PEO 37 -PPO 56 -PEO 37 triblock copolymer with high MDR tumor-sensitizing ability, and Pluronic F127 is presented as PEO 100 -PPO 65 -PEO 100 triblock copolymer with long poly(ethylene oxide) (PEO) hydrophilic chain which can improve the physical stability and increase the blood circulation time in vivo of the polymeric micelles. Compared to single Pluronic P105 micelles, the micelles comprising Pluronic P105 and F127 can be more stable, due to the lowered critical micelle concentration conferred by the high PEO-poly(propylene oxide) ratio of F127, which will contribute to the long retention of drug-loaded micelles in blood, and eventually achieve higher drug accumulation at the target site (tumor) through the EPR effect.…”

“…Endocytosis occurs in most cells as pinocytosis, and normally represents four basic mechanisms: clathrin-mediated endocytosis, caveolae-mediated endocytosis, macropinocytosis, as well as both clathrin-and caveolae-independent endocytosis. 35 The effect of clathrin-mediated endocytosis on the internalization of the mixed micelles was evaluated using CPZ and sucrose, [36][37][38] a kind of clathrin-coated pits formation blocking agent. Filipin and genistein were used to evaluate the effect of caveolae-mediated endocytosis on the cellular uptake.…”

Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells

“…1,9,26,[40][41][42][43][44] Figure 1 shows a comparison of the kinetics of uptake of silica nanoparticles in A549 cells in serum free and complete media, obtained by flow cytometry, as described in the Methods. As anticipated in Supplementary Figure S1, the kinetic study also clearly indicated that uptake was always higher when the nanoparticles were exposed to cells in SF.…”

PLGA-Based Nanoparticles as Cancer Drug Delivery Systems

“…Cationic surface charge is desirable as it promotes interaction of the nanoparticles with the cells and thus augments the rate and extent of internalization (Shenoy et al, 2005;Kumari et al, 2010). PLGA nanoparticles have negative charges which can be changed to neutral or positive charges by surface modification, for instance by PEGylation of the PLGA polymer or chitosan coating respectively (Tahara et al, 2009;Danhier et al, 2010;Danhier et al, 2012).…”