Improved Callus Quality and Prolonged Regenerability in Model and Recalcitrant Barley (Hordeum vulgare L.) Cultivars.

Jiang, Wenjun; Cho, Myeong‐Je; Lemaux, Peggy G.

doi:10.5511/plantbiotechnology.15.63

Cited by 29 publications

(26 citation statements)

References 19 publications

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“…The highest initiation of callus was on the medium with dicamba in five varieties and on the medium with 2,4-D in three varieties. Similarly, Jiang et al (1998) tested two barley cultivars Golden Promise and the recalcitrant commercial cultivar Galena and found out that two auxins (dicamba and 2,4-D) alone were the best of all tested phytohormones because callus induction frequencies were nearly 100%. Bregitzer (1992) also observed that callus formation was affected by genotype, medium, and their interaction.…”

Section: Discussionmentioning

confidence: 99%

“…The composition of the media, including growth regulators, is another important factor influencing culture initiation and plant regeneration of immature embryos. Phytohormones are crucial to establishing optimal culture conditions since they play a pivotal role in producing relatively undifferentiated callus tissue from differentiated tissues such as an embryo (Jiang et al 1998). The auxin 2,4-dichlorophenoxyacetic acid (2,4-D), by itself or in combination with cytokinins, has been widely used to enhance callus induction Callus induction and regeneration efficiency of spring barley cultivars registered in the Czech Republic and maintenance (Castillo et al 1998).…”

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confidence: 99%

“…The auxin 2,4-dichlorophenoxyacetic acid (2,4-D), by itself or in combination with cytokinins, has been widely used to enhance callus induction Callus induction and regeneration efficiency of spring barley cultivars registered in the Czech Republic and maintenance (Castillo et al 1998). Both are required for cell division and during the in vitro culture, the balance between the two hormones determines what developmental tissue types are formed from the cell divisions (Jiang et al 1998). …”

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Callus induction and regeneration efficiency of spring barley cultivars registered in the Czech Republic

Šerhantová¹,

Ehrenbergerová²,

Ohnoutková³

2004

PLANT SOIL ENVIRON

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Immature zygotic embryos are frequently used for induction of cell cultures in vitro and for genetic transformation. The effect of three synthetic auxins: 2,4-dichlorophenoxyacetic acid (2,4-D), 3,6-dichloro-2-methoxybenzoic acid (dicamba) and 4-amino-3,5,6-trichloropicolinic acid (picloram) on induction and subsequent regeneration capacity of barley. In vitro culture was studied in 12 spring barley cultivars registered in the Czech Republic and a variety of Golden Promise, with known high regeneration efficiency. The callus formation frequency and number of green regenerants were influenced significantly both by genotype and auxin. Most cultivars formed statistically significantly a higher mean number of regenerants (1.95) a�er the callus induction on the medium with 2.4-D as compared to the media with picloram and dicamba. Only two cultivars (Nordus and SG-S-261) did not respond to the used auxins differently. The highest average number of regenerants (from all three auxins) was obtained with Golden Promise (2.7 plants per one cultivated scutellum). From the set of genotypes used in our study, the cultivars Atribut, Forum, and Scarle� with the mean number of regenerants (2.09-1.57) would be the most suitable cultivars for further transformation use. They differ statistically significantly from the cultivars Nordus, Amulet, Akcent, SG-S-252, Orbit, and Granát (0.42-0.92) which had the lowest mean number of regenerants.

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Section: Discussionmentioning

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Callus induction and regeneration efficiency of spring barley cultivars registered in the Czech Republic

Šerhantová¹,

Ehrenbergerová²,

Ohnoutková³

2004

PLANT SOIL ENVIRON

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“…Using standard in vitro protocols, cultured barley tissues typically lose much of their regenerative capacity in that timeframe (Ziauddin and Kasha, 1990;Hang and Bregitzer, 1993;Bregitzer et aI., 1995a;Jiang et al, 1998).…”

Section: Development Of Long-term Green Regenerative Tissuesmentioning

confidence: 99%

Transgenic Cereals: Hordeum vulgare L. (barley)

Lemaux

Cho

Zhang

et al. 1999

Advances in Cellular and Molecular Biology of Plants

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The development of barley as a crop dates to the earliest agricultural activities of humans, and it remains one of the major cereals grown for feed and food, and for the production of beer. In this century, an understanding and application of quantitative genetic theory has created a genetically elite crop that is divergent from its ancestors. Further improvements in barley cultivars will depend on continued access to useful allelic variability. Sexual hybridization will continue to play an important role in such improvement, but its utility is limited because potentially useful alleles are either linked to undesirable alleles or unavailable because of sexual incompatibilty. The advent of molecular genetics and nonsexual gene transfer offers exciting opportunities to bypass these limitations and to provide access to more diverse sources of genes. Recent developments have added barley to the list of major crops that are amenable to this type of genetic manipulation either through direct DNA transfer (bombardment) or mediated by Agrobacterium tumefaciens. However, significant problems remain, and include: I) the lack of reproducible, efficient transformation systems for commercial germplasm; 2) the induction of stable genetic and epigenetic changes during the in vitro process; and 3) transgene and transgene expression instability. In this chapter, we will discuss and describe the first systems used for the genetic transformation of barley, introduce and describe the development of new systems for barley transformation, and comment on past and future uses of barley transformation as a tool for basic science and commercial application.IX Vasil (ed.;. Molecular Improvement o/Cereal Crops. 255 -316

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“…Media composition-mainly the hormonal balance-is an important factor influencing in vitro culture initiation and plant regeneration from embryos [11]. The auxin 2, 4-dichlorophenoxy acetic acid (2, 4-D) alone or in combination with cytokinins, is widely used to enhance callus induction and maintenance [12].…”

Section: Introductionmentioning

confidence: 99%

Callus Induction, Proliferation, and Plantlets Regeneration of Two Bread Wheat (Triticum aestivumL.) Genotypes under Saline and Heat Stress Conditions

et al. 2012

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Response of two genotypes of bread wheat (Triticum aestivum), Mahon-Demias (MD) and Hidhab (HD1220), to mature embryo culture, callus production, and in vitro salt and heat tolerance was evaluated. For assessment of genotypes to salt and heat tolerance, growing morphogenic calli were exposed to different concentrations of NaCl (0, 5, 10, and 15 g·L −1 ) and under different thermal stress intensities (25, 30, 35, and 40 • C). Comparison of the two genotypes was reported for callus induction efficiency from mature embryo. While, for salt and heat tolerance, the proliferation efficiency, embryonic efficiency, and regeneration efficiency were used. The results show significant medium and genotype effects for the embryogenesis capacity of calluses induction and plantlets regeneration under saline and thermal stresses. Mahon-Demias showed good callus induction and ability to proliferate and regenerate seedling under heat and salt stress conditions compared to Hidhab. No sizeable differences were observed between the two genotypes at higher salt stress rates. This study will serve as a base line for in vitro screening of several elite wheat cultivars for their ability to induce callus and regenerate plants from mature embryos, and to start selection for tolerance to salinity.

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Improved Callus Quality and Prolonged Regenerability in Model and Recalcitrant Barley (Hordeum vulgare L.) Cultivars.

Cited by 29 publications

References 19 publications

Callus induction and regeneration efficiency of spring barley cultivars registered in the Czech Republic

Callus induction and regeneration efficiency of spring barley cultivars registered in the Czech Republic

Transgenic Cereals: Hordeum vulgare L. (barley)

Callus Induction, Proliferation, and Plantlets Regeneration of Two Bread Wheat (Triticum aestivumL.) Genotypes under Saline and Heat Stress Conditions

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