Improved Bovine Blastocyst Developmental Potential by L-carnitine Supplementation

Manzano, Paul Christian Ver J.; Ocampo, Marlon B.; Ocampo, Lerma C.; Maylem, Excel Rio S.; Lazaro, Jonathan V.

doi:10.12983/ijsrk-2015-p0021-0029

Cited by 6 publications

(7 citation statements)

References 27 publications

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“…The improvementin the in vitro fertilization and conception ratesrecorded in the 0.05mg/ml Lcarnitine treated semen might be emphasized by increased ATP production and antioxidant effect of lcarnitine. These results are consistent with Rizzo et al (2010) and Manzano et al (2015) who reported that LC have a beneficial roles on mammalian embryos metabolism and development; through its contribution in lipid and energy metabolism, as well as its antioxidant effects by enhancing the activity of numerous antioxidant enzymes in addition to its antiapoptotic effect.…”

Section: Discussionsupporting

confidence: 92%

Effects of L-Carnitine on Morphology and Antioxidant Enzymes and Dna Integrity of Cryopreserved Buffalo Spermatozoa

2017

Assiut Veterinary Medical Journal

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Cryopreservation induces sub lethal damage to the spermatozoa, thereby reduce their fertile life. There are some biochemical additives that may enhance buffalo semen freezability; L-carnitine is one of these biochemical semen additives. Till now, the exact effects of L-carnitine on buffalo semen processing outcomes haven't been discovered. The current study aimed to clarify L-carnitine roles during buffalo semen cryopreservation. Semen was cryopreserved in tris-based extender supplemented with different concentrations of L-carnitine (0.01, 0.05 and 0.1mg/ml)Vs.Tris-based extender only (control). Then they were processed to cryopreservation and thawing to assess different semen characteristics. Cryopreserved semen was assessed for percentage post-thawing motility, acrosomal and plasma membrane integrity, viability, DNA damage, antioxidant enzymes concentration, lipid peroxidation, in vitro fertilizing potentials and conception rate. Current results indicated that addition of 0.05 mg/ml L-carnitine to semen extender significantly (P<0.05) improved post-thawing motility, viability and acrosomal integrity (63.33±9.28 %, 133.33±9.40 and 12.33± 2.02 %, respectively) compared with control (43.33±6.01 %, 74.16± 10.93 and 24.67±2.03 %, respectively). Moreover, 0.05mg/ml L-carnitine significantly increased (P<0.05) total antioxidant capacity (TAC), superoxide dismutase (SOD) and glutathione peroxidase (GPx) concentrations (0.50±0.07 mµ/ml, 73.67±5.37 U/ml and 106.66±12.03 U/L, respectively) with respect to the control (0.19±0.01 mµ/ml, 27.33±3.49 U/ml and52.33±4.09 U/L, respectively). Furthermore, 0.05mg/ml Lcarnitine significantly decreased (P<0.05) lipid peroxidation of the cryopreserved spermatozoa compared with the control semen (11.00±1.73 vs 24.82±4.90nmol/ml). Likewise, at this concentration sperm DNA damage, tail length and tail moment of the cryopreserved semen significantly (P<0.05) reduced (1.87±0.36 %, 1.83±0.33 µm and 3.72±1.44, respectively) compared with control (3.47±0.13 %, 3.48±0.17 µm and 11.91±0.87, respectively). Additionally, 0.05 mg/ml L-carnitine significantly (P<0.05) improved in vitro fertilization rate (57.45%) and conception rate (61.76%) compared with the control (33.33 and 37.93%, respectively). In conclusion the use of 0.05 mg/ml L-carnitine in the freezing extender improves DNA integrity through enhancing the antioxidant defense of buffalo sperm cells and decreasing the rate of lipid peroxidation. Therefore, L-carnitine may improve sperm cryopreservation quality, reduce cryodamage and improve sperm fertilizing potential.

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Section: Discussionsupporting

confidence: 92%

Effects of L-Carnitine on Morphology and Antioxidant Enzymes and Dna Integrity of Cryopreserved Buffalo Spermatozoa

2017

Assiut Veterinary Medical Journal

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“…They reported improved developmental potential up to the blastocyst stage with higher blastocyst formation rate and increased total cell count and activity when the oocytes were matured in culture media added with 0.1–0.5 mg/mL LC. In a subsequent experiment, supplementation of the resulting zygotes with the same dose of LC in modified synthetic oviductal fluid medium displayed a higher blastocyst total cell count, but similar blastocyst formation rates as that of control [ 70 ].…”

Section: Introductionmentioning

confidence: 99%

Role of L-carnitine in female infertility

Agarwal

Sengupta

Durairajanayagam

2018

Reprod Biol Endocrinol

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BackgroundL-carnitine (LC), and its acetylated form, acetyl L-carnitine (ALC), have immense functional capabilities to regulate the oxidative and metabolic status of the female reproductive system. The vulnerability of this system to free radicals demand for advanced strategies to combat them. For this purpose, the ‘quasi vitamins’ LC and ALC can be used either individually, or in combination with each other or with other antioxidants.Main bodyThis review (a) summarizes the effects of carnitines on female fertility along with the findings from various in vivo and in vitro studies involving human, animal and assisted reproductive technology, and (b) proposes their mechanism of actions in improving female fertility through their integrated actions on reducing cellular stress, maintaining hormonal balance and enhancing energy production. They reportedly aid β-oxidation in oocytes, maintain its cell membrane stability by acetylation of phospholipids and amphiphilic actions, prevent free radical-induced DNA damage and also stabilize acetyl Co-A/Co-A ratio for adequate acetyl storage as energy supply to maintain the robustness of reproductive cells.ConclusionWhile both LC and ALC have their applications in improving female fertility, ALC is preferred for its better antioxidant properties and LC for amelioration of energy supply to the cells. These beneficial effects show great promise in its application as a treatment option for women facing infertility disorders.

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“…The current study indicated significant improvement in fertilization in vitro and developmental competence of embryos to reach blastocyst stage due to treatment of rabbit does with CoQ10 or LC. In this respect, Manzano et al (2015) found that LC supplementation was effective at supporting blastocyst formation and improving its quality as evidenced by having a higher total cell count. Addition of LC to culture medium enhanced lipid metabolism and mitochondrial activity and decreased ROS levels, as well as improves MII and cleavage rates (Somfai et al, 2011), although the treatment does not impact blastocyst formation.…”

Section: Maturation Fertilization and Embryonic Stages Of Follicularmentioning

confidence: 98%

“…Dunning et al (2011) found that oocytes isolated from the follicles treated with LC had greater rates of maturity (metaphase II/MII), higher fertilization rates and improved blastocyst development. Also, Manzano et al (2015) reported that LC supplementation was effective at supporting blastocyst formation and improving its quality as evidenced by having a higher total cell count.…”

Section: Maturation Fertilization and Embryonic Stages Of Follicularmentioning

confidence: 99%

“…Recently, Manzano et al (2015) showed that nuclear maturation and cleavage rate of COCs were not influenced by the presence of LC during the initial maturation period, but LC supplementation was effective at supporting blastocyst formation and improving its quality as evidenced by having a higher total cell count. In this respect, You et al (2012) mentioned that LC plays an important role in the lipids/fatty acid metabolism as an endogenous energy source for the oocytes and embryos.…”

Section: Introductionmentioning

confidence: 99%

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Litter Size, Ovarian Characteristics, and Oocyte in Vitro Maturation and Fertilization of Rabbits Administrated With Coenzyme Q10 and L-Carnitine

Younan

El-Nagar

Wafa

et al. 2015

Egyptian Journal of Nutrition and Feeds

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he objective of this study was to evaluate the effect of coenzyme Q10 (CoQ10) and L-carnitine (LC) administration on litter size, ovarian characteristics and in vitro production of rabbit embryos. This study was carried out at the International Livestock Management Training Center (ILMTC), belonging to the Animal Production Research Institute, Agricultural Research Center, Ministry of Agriculture. Total of 36 mature NZW rabbit does (5-6 months of age, 3-3.5 kg LBW as well as 6 NZW bucks (7.5-8 months of age and 3.5-4.0 kg LBW) were used in this study. Does were divided into 3 similar groups, (n=12). The 1 group was control (G1), while does in the 2 and 3 groups were given daily oral dose of 10 mg CoQ10/kg LBW (G2) and 40 mg LC/kg LBW (G3) for 21 days prior to natural mating, respectively. Five does from each group were slaughtered post-mating as oocyte donors for studying the effect of treatments on ovarian characteristics and in vitro maturation and fertilization of oocytes. Immediately after slaughtering, ovaries were removed and oocytes were collected by slicing technique and evaluated, then only compactcumulus oocytes (COCs) were matured and fertilized in vitro. For the rest number of does in each group (n=7), pregnancy was handy diagnosed by palpation 10-12 days post-mating. Also, litter size and weight at birth up to weaning were recorded. The obtained results showed that, ovarian weight and number of follicles increased (P<0.05) in G2 and G3. Number of bleeding follicles and recovered oocytes increased (P<0.05) only in G2 as compared to G1, however, oocyte recovery rate was not affected by treatment. Frequency distribution of compact and partial denuded oocytes was higher (P<0.05), while that of expanded and denuded oocytes was lower in G2 and G3 as compared to G1. Percentage of oocytes with full expansion (maturation rate) was 80.3 and 79.3% vs. 73.8% and fertilization rate was 67.4 and 66.7% vs. 64.5% in G2 and G3 vs. G ((P≥0.05). Percentage of embryos at morula stage was higher (P<0.05) in G2 and G3 than in G1 (30.3 and 3.1% vs. 20%), respectively. Percentage of embryos at blastocyst stage was 21.2 and 21.4% vs. 15% in G2 and G3 vs. G1, respectively. Litter size of rabbit does was higher (P<0.05) in G2 and G3 than in G1 at birth (7.29 and 6.71 vs. 5.43/doe), at 21 days of age (6.71 and 5.86 vs. 4.43/doe) and at weaning (6.43 and 5.43 vs. 4.14/doe). Mortality rate of kits at birth or weaning was not affected by treatment. Average kit weight at birth was higher (P<0.05) in G2 and G1. However, average kit weight at weaning, average litter weight from birth up to weaning and average litter daily gain from birth up to weaning were higher (P<0.05) in G2 and G3 than in G1. In conclusion, treatment of rabbit does 21 days prior to insemination with CoQ10 at a level of 10 mg/kg LBW or L-carnitine at level 40 mg/kg LBW as daily oral dose is recommended to improve in vitro embryo production and also to increase litter characteristics (size and weight from birth up to weaning) of New Zealand White rabbit does.

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Improved Bovine Blastocyst Developmental Potential by L-carnitine Supplementation

Cited by 6 publications

References 27 publications

Effects of L-Carnitine on Morphology and Antioxidant Enzymes and Dna Integrity of Cryopreserved Buffalo Spermatozoa

Effects of L-Carnitine on Morphology and Antioxidant Enzymes and Dna Integrity of Cryopreserved Buffalo Spermatozoa

Role of L-carnitine in female infertility

Litter Size, Ovarian Characteristics, and Oocyte in Vitro Maturation and Fertilization of Rabbits Administrated With Coenzyme Q10 and L-Carnitine

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