Improved bio-catalytic conversion by novel immobilization process using cryogel beads to increase solvent production

Tripathi, Anuj; Sami, Haider; Jain, Siddhant; Viloria-Cols, Maria; Zhuravleva, N. V.; Nilsson, Göran N.; Jungvid, Hans; Kumar, Ashok

doi:10.1016/j.enzmictec.2010.03.009

Cited by 66 publications

(35 citation statements)

References 18 publications

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“…Polyvinyl alcohol (PVA) cryogels formed by the freeze-thawing method, for example, have been widely used for immobilization of whole cells [48]. However, free enzymes, owing to their smaller size, can diffuse out of the gel matrix and are, consequently, leached in an aqueous medium.…”

Section: Hydrogelsmentioning

confidence: 99%

Enzyme immobilization: an update

et al. 2013

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Compared to free enzymes in solution, immobilized enzymes are more robust and more resistant to environmental changes. More importantly, the heterogeneity of the immobilized enzyme systems allows an easy recovery of both enzymes and products, multiple re-use of enzymes, continuous operation of enzymatic processes, rapid termination of reactions, and greater variety of bioreactor designs. This paper is a review of the recent literatures on enzyme immobilization by various techniques, the need for immobilization and different applications in industry, covering the last two decades. The most recent papers, patents, and reviews on immobilization strategies and application are reviewed.

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Section: Hydrogelsmentioning

confidence: 99%

Enzyme immobilization: an update

et al. 2013

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“…Various immobilization matrices were screened in batch fermentation. 63,65,66 Survase et al 66 recently reported that the addition of support matrix improves the substrate consumption and its conversion to solvents.…”

Section: Immobilization Materials In Fermentationmentioning

confidence: 99%

“…There are various reports on different types of immobilization materials ranging from synthetic to biodegradable polymers and fibers. Tripathi et al 63 suggested agarose-alginate cryogel beads after comparing with the other potential support matrices such as coconut fibers, brick pieces and burnt coal. Efremenko et al 64 showed that the immobilization of Clostridium cells using poly(vinyl alcohol) cryogel changed the ratio of ABE solvents (4:12:1) produced resulting higher butanol titers.…”

Section: Immobilization Materials In Fermentationmentioning

confidence: 99%

Biobutanol: the outlook of an academic and industrialist

et al. 2013

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The gradual shift of transportation fuels from oil based fuels to the alternative fuel resources and worldwide demand for energy has been the impetus for research to produce alcohol biofuels from renewable resources. Current bioethanol and biodiesel can, however, not cover an increasing demand for biofuels. Hence, there is an extensive need for advanced biofuels with superior fuel properties. The present review is focused on the developments of biobutanol, which is regarded to be superior to bioethanol in terms of energy density and hygroscopicity. Although acetone-butanolethanol (ABE) fermentation is one of the oldest large-scale fermentation processes, butanol yield by anaerobic fermentation remains sub-optimal. For sustainable industrial scale butanol production, a number of obstacles need to be addressed including choice of feedstock, low product yield, product toxicity to production strain, multiple end-products and downstream processing of alcohol mixtures.Metabolic engineering provides a means for fermentation improvements. Different strategies are employed in the metabolic engineering of Clostridia that aim to enhance the solvent production, improve selectivity for butanol production, and increase the tolerance of Clostridia to solvents. The introduction and expression of a non-clostridial butanol producing pathway in E. coli is most promising strategy for butanol biosynthesis. Several rigorous kinetic and physiological models for fermentation have been formulated, which form useful tool for optimization of the process. Due to the lower butanol titers in the fermentation broth, simultaneous fermentation and product removal techniques have been developed to improve production economics. With the use of new strains, inexpensive substrates, and superior reactor designs, economic ABE fermentation may further attract an attention of researchers all over the world. The present review is attempting to provide an overall outlook on discoveries and strategies that are being developed for commercial n-butanol production.

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“…The production medium reported by Tripathi et al [25] was modified to use a sugar mixture as a carbon source containing glucose, mannose, arabinose, galactose, and xylose as a replacement to sole glucose (60 g L -1 ). The artificial sugar mixture equivalent to SEW spent liquor contained (g L -1 ) glucose 32.73, mannose 14.78, arabinose 2.14, galactose 3.95, and xylose 6.41 to get total sugars to be 60 g L -1 .…”

Section: Microorganism and Mediummentioning

confidence: 99%

Enhanced isopropanol–butanol–ethanol (IBE) production in immobilized column reactor using modified Clostridium acetobutylicum DSM792

Bankar

Jurgens

Survase

et al. 2014

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Highlights Metabolic engineering to convert acetone into isopropanol  Continuous immobilized fermentation to validate industrial feasibility of strain  Optimization of bioprocess to enhance the solvent yield and productivity  Utilization of lignocellulosic spent liquor as a substrate for the fermentation AbstractThe co-production of acetone during traditional butanol fermentation (acetone-butanol-ethanol) lowers the yield of alcohol biofuels. The present study was aimed to develop an improved Clostridium acetobutylicum strain with enhanced alcohol fuel production capability. The previously developed IBE producing C. acetobutylicum DSM792-ADH strain was further optimized to enhance the solvent productivity and yield. Immobilized cell column reactor was used to investigate the effect of dilution rate on IBE fermentation. Pure glucose, artificial sugar mixture and SEW spent liquor from spruce chips were used as substrates to study the behavior of the modified microorganism. The highest total solvent concentrations of approximately10.60 g.l -1 , 10 g.l -1 and 6 g.l -1 were obtained when glucose, sugar mixture and SEW spent liquor were used as substrate respectively. The modified microorganism could effectively utilize the lignocellulosic biomass hydrolyzate (SEW spent liquor) to yield a solvent productivity of 1.67 g.l -1 .h -1 .

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Improved bio-catalytic conversion by novel immobilization process using cryogel beads to increase solvent production

Cited by 66 publications

References 18 publications

Enzyme immobilization: an update

Enzyme immobilization: an update

Biobutanol: the outlook of an academic and industrialist

Enhanced isopropanol–butanol–ethanol (IBE) production in immobilized column reactor using modified Clostridium acetobutylicum DSM792

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