Improved bacterial growth test for rapid water toxicity screening

Slabbert, J. L.

doi:10.1007/bf01607805

Cited by 30 publications

(9 citation statements)

References 5 publications

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“…In a MetPLATE ™ assay system (based on β-galactosidase activity), zinc IC 50 of 0.11 ± 0.001 mg/l (0.002mM) was reported for Escherichia coli [5]. In a growth inhibition test assessed via turbidity measurements, 7.15 mg/l Zn 2+ (0.12mM) inhibited the growth of Pseudomonas putida by 50 % [34]. In The result of the in vitro study indicated that Zn 2+ is potentially toxic to the sediment bacteria of New Calaber River.…”

Section: +mentioning

confidence: 99%

Toxicity of Zinc to Heterotrophic Bacteria From a Tropical River Sediment

Nweke¹

2007

Appl Ecol Env Res

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Abstract. Tolerance to Zn 2+ by pure cultures of Bacillus, Salmonella and Arthrobacter species isolated from New Calabar River sediment was assessed through dehydrogenase assay. The cultures were exposed to Zn 2+ concentrations of 0.2 to 2.0 mM in a nutrient broth-glucose-TTC medium. The responses of the bacterial strains varied with Zn 2+ concentration. In Salmonella sp. SED2, Zn 2+ stimulated dehydrogenase activity at 0.2 mM. In Bacillus sp. SED1 and Arthrobacter sp. SED4, dehydrogenase activity was progressively inhibited with increasing Zn 2+ concentration. The IC 50 ranges from 0.206 ± 0.030 to 0.807 ± 0.066 mM. Total inhibition of dehydrogenase activity was observed at concentrations ranging from 1.199 ± 0.042 to 1.442 ± 0.062. The order of zinc tolerance is: Salmonella sp. SED2 > Arthrobacter sp. SED4 > Bacillus sp. SED1. The result of the in vitro study indicated that Zinc is potentially toxic to sediment bacteria and could pose serious threat to their metabolism in natural environments.

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Section: +mentioning

confidence: 99%

Toxicity of Zinc to Heterotrophic Bacteria From a Tropical River Sediment

Nweke¹

2007

Appl Ecol Env Res

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“…The Pseudomonas putida (P. putida) strain was obtained from the Bacterial Culture Collection of the Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. The bacterial growth inhibition test was carried out according to the methodology proposed by Slabbert [14] with a few modifications (sample volume increased and water added to dilute the test receptacles). An overnight culture of P. putida and E. coli, prepared individually, was diluted with fresh medium (Nutrient Broth 40 g/L) to an optical density (OD) of approximately 0.8.…”

Section: Growth Inhibition Tests With Bacteria (E Coli and P Putida)mentioning

confidence: 99%

Ecotoxicological evaluation of waste water from nitrocellulose production

Ribeiro

Silva

Paiva

2013

Journal of Environmental Science and Health, Part A

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The present study evaluated the toxic potential and physicochemical characteristics of waste water generated in nitrocellulose production, including effluents from delignification, bleaching, nitration and mixture (composed of these three effluents), from a plant in the Paraiba Valley, São Paulo, Brazil. The test organisms used for toxicity assays were Daphnia similis, Danio rerio, Escherichia coli, Pseudomonas putida and Pseudokircheneriella subcaptata. The results showed that the effluents analyzed present acute and chronic toxicity for the organisms tested. Nitration effluent was the least toxic, while delignification and bleaching effluents were the most toxic. The naturally occurring pollutants in wood fiber and cotton, like lignin, probably contributed to effluents toxicity, in addition to higher concentrations of total dissolved solids, which are in non-compliance with Brazilian legislation, conductivity, chemical oxygen demand and color. The discharge of nitrocellulose effluent into an aquatic environment should only occur after appropriate treatment, due to their toxic characteristics.

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“…Although not developed specifically for the testing of freshwaters, a number of bioassays based on the inhibition by toxicants of bacterial growth have Ž been described previously Alsop et al, 1980;Trevors . et al, 1981;Slabbert, 1986 . One particular test in which the inhibition of the growth of Pseudomonas putida by as little as 10 grL of copper could be detected within 6 h, is not only sensitive, but also very Ž .…”

Section: Introductionmentioning

confidence: 96%

“…One particular test in which the inhibition of the growth of Pseudomonas putida by as little as 10 grL of copper could be detected within 6 h, is not only sensitive, but also very Ž . rapid Slabbert, 1986 . Alternative rapid approaches have focussed on the inhibition by toxicants of the uptake of radiolabelled substrates such as 14 C-glucose…”

Section: Introductionmentioning

confidence: 97%