Improved axial resolution of FINCH fluorescence microscopy when combined with spinning disk confocal microscopy

Siegel, Nisan; Brooker, Gary

doi:10.1364/oe.22.022298

Cited by 32 publications

(20 citation statements)

References 16 publications

(27 reference statements)

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“…51 Several months after the appearance of the first confocal FINCH, 44 another confocal microscope was proposed, combining standard FINCH with a spinning disk. 52 However, note that the original confocal FINCH 44 is motionless and accomplishes optical sectioning of the observed specimen without losing the inherent transverse super-resolution capabilities of the original FINCH. 21 Therefore, confocal FINCH can image different planes of interest at various depths while blocking light from other planes.…”

Section: Confocal Fresnel Incoherent Correlation Holographymentioning

confidence: 99%

Incoherent digital holography with phase-only spatial light modulators

Rosen

Kelner

Kashter

2015

J. Micro/Nanolith. MEMS MOEMS

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Abstract. Today, spatial light modulators (SLMs) offer the world of digital holography a robust technology that can be incorporated into hologram recorders. This review surveys recent developments related to the role of SLMs in a family of incoherent digital hologram recorders termed Fresnel incoherent correlation holography (FINCH). Two systems branching out from FINCH, and discussed herein, are a confocal version of FINCH and a synthetic aperture FINCH-based system.

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Section: Confocal Fresnel Incoherent Correlation Holographymentioning

confidence: 99%

Incoherent digital holography with phase-only spatial light modulators

Rosen

Kelner

Kashter

2015

J. Micro/Nanolith. MEMS MOEMS

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“…The widefield FINCH or confocal CINCH holographic image is likewise imaged by camera 1 depending upon the spinning disk placement after the image beam passes through lens L4, active GRIN lens G1, inactive GRIN lens G2, liquid crystal phase shifter ϕ and output polarizing filter (Pol.). From Reference 17.…”

Section: Figurementioning

confidence: 99%

“…The CINCH phase images contain the depth dependent phase information derived from the CINCH holograms and the reconstructed CINCH images show the complex holograms propagated to the best focal plane. From Reference 17.…”

Section: Figurementioning

confidence: 99%

“…In certain applications it would be desirable to reduce or eliminate those out-of-focus contributions while maintaining the inherent FINCH super-resolution advantage, resulting in a final high quality FINCH image of a single plane with super-resolution and low background. Recently we have accomplished that goal by incorporating our commercial CARV confocal excitation/emission Nipkow disk unit with our newly developed high efficiency transmissive FINCH system to create an instrument that exceeds the overall XYZ resolution of FINCH and confocal microscopy 17 . In that paper 17 and with a multi-dye specimen shown in this communication, we demonstrate the efficacy of that system to create high resolution confocal images and confocal FINCH holograms of a biological sample at high magnification and with high NA objectives, with exposure times of milliseconds to seconds depending upon the brightness of the sample.…”

Section: Introductionmentioning

confidence: 99%

“…Recently we have accomplished that goal by incorporating our commercial CARV confocal excitation/emission Nipkow disk unit with our newly developed high efficiency transmissive FINCH system to create an instrument that exceeds the overall XYZ resolution of FINCH and confocal microscopy 17 . In that paper 17 and with a multi-dye specimen shown in this communication, we demonstrate the efficacy of that system to create high resolution confocal images and confocal FINCH holograms of a biological sample at high magnification and with high NA objectives, with exposure times of milliseconds to seconds depending upon the brightness of the sample. For simplicity we call the confocal FINCH technique CINCH for C onfocal Inc oherent H olography.…”

Section: Introductionmentioning

confidence: 99%

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CINCH (confocal incoherent correlation holography) super resolution fluorescence microscopy based upon FINCH (Fresnel incoherent correlation holography)

Siegel

Storrie

Bruce³

et al. 2015

SPIE Proceedings

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FINCH holographic fluorescence microscopy creates high resolution super-resolved images with enhanced depth of focus. The simple addition of a real-time Nipkow disk confocal image scanner in a conjugate plane of this incoherent holographic system is shown to reduce the depth of focus, and the combination of both techniques provides a simple way to enhance the axial resolution of FINCH in a combined method called “CINCH”. An important feature of the combined system allows for the simultaneous real-time image capture of widefield and holographic images or confocal and confocal holographic images for ready comparison of each method on the exact same field of view. Additional GPU based complex deconvolution processing of the images further enhances resolution.

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A simple, inexpensive and multi‐scale 3‐D fluorescent test sample for optical sectioning microscopies

Olevsko

Szederkenyi

Corridon

et al. 2021

Microscopy Res & Technique

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Fluorescence standards allow for quality control and for the comparison of data sets across instruments and laboratories in applications of quantitative fluorescence. For example, users of microscopy core facilities can expect a homogenous and time‐invariant illumination and an uniform detection sensitivity, which are prerequisites for imaging analysis, tracking or fluorimetric pH or Ca2+‐concentration measurements. Similarly, confirming the three‐dimensional (3‐D) resolution of optical sectioning microscopes calls for a regular calibration with a standardized point source. The test samples required for such measurements are typically different ones, they are often expensive and they depend much on the very microscope technique used. Similarly, the ever‐increasing choice among microscope techniques and geometries increases the demand for comparison across instruments. Here, we advocate and demonstrate the multiple uses of a surprisingly versatile and simple 3‐D test sample that can complement existing and much more expensive calibration samples: commercial tissue paper labeled with a fluorescent highlighter pen. We provide relevant sample characteristics and show examples ranging from the sub‐μm to cm scale, acquired on epifluorescence, confocal, image scanning, two‐photon (2P) and light‐sheet microscopes.

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Improved axial resolution of FINCH fluorescence microscopy when combined with spinning disk confocal microscopy

Abstract: FINCH holographic fluorescence microscopy creates superresolved images with enhanced depth of focus. Addition of a Nipkow disk real-time confocal image scanner is shown to reduce the FINCH depth of focus while improving transverse confocal resolution in a combined method called "CINCH".

Cited by 32 publications

References 16 publications

Incoherent digital holography with phase-only spatial light modulators

Incoherent digital holography with phase-only spatial light modulators

CINCH (confocal incoherent correlation holography) super resolution fluorescence microscopy based upon FINCH (Fresnel incoherent correlation holography)

A simple, inexpensive and multi‐scale 3‐D fluorescent test sample for optical sectioning microscopies

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