Improved assay method for the determination of pyronaridine in plasma and whole blood by high-performance liquid chromatography for application to clinical pharmacokinetic studies

JPC-2997, a New Aminomethylphenol with High In Vitro and In Vivo Antimalarial Activities against Blood Stages of Plasmodium

“…It appears to be well absorbed and exhibits biphasic decline in both blood and plasma concentrations, with a relatively long elimination half-life of 49.8 h. JPC-2997's elimination half-life is longer than those of dihydroartemisinin (25 min) (23), amodiaquine (ϳ7 h) (24), atovaquone (12 h) (25), and mefloquine (17 h) (26), and comparable to that of chloroquine (47 h) (27) but shorter than that of piperaquine (15 days) (28). Unlike the quinoline antimalarials amodiaquine (29), chloroquine (30), and pyronaridine (31), which concentrate in erythrocytes, JPC-2997 does not appear to accumulate in erythrocytes, with a blood-to-plasma concentration ratio of 0.7. However, accurately measuring JPC-2997's uptake into erythrocytes requires not only measurement of the blood-to-plasma concentration ratio but also knowledge of the unbound fraction of JPC-2997 in plasma, which is presently unknown, and the hematocrit of the blood sample.…”

A new and simple solid-phase extraction method for LC determination of pyronaridine in human plasma

“…In this study the limit of quantification was 0.010 g/mL plasma, which was better than that reported by Chen and Fleckenstein (about 0.028 g/mL) who used whole blood and plasma. A recent automated solid-phase extraction method for whole blood also did not offer a better limit of quantification (about 0.025 g/mL) [8]. In addition, the method requires blood haemolysis and sample buffering before loading onto the SPE column.…”

Comparison of the pharmacokinetics of moxidectin and ivermectin after oral administration to beagle dogs

“…Moxidectin and ivermectin were analysed by high performance liquid chromatography (HPLC), according to the methods previously described for ivermectin [11] and moxidectin [12]. Acetonitrile (100 ml) and water (40 ml) were added to 200 ml of plasma.…”