Improved artificial diet for mass rearing of the tobacco caterpillar, Spodoptera litura (Lepidoptera: Noctuidae)

Gupta, G. N.; Rani, Seema; Birah, Ajanta; Raghuraman, M.

doi:10.1079/ijt200551

Cited by 56 publications

(45 citation statements)

References 3 publications

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“…General growth conditions for larvae before and during the experiments were 26°C, 60% humidity and a 16-h day, 8-h night cycle. Before experiments, boxes with larvae were placed at 18°C in the dark for 48 h. For virulence assays, 1-week-old larvae were exposed to 10 μl washed bacterial cells adjusted to the desired concentration or 0.9% NaCl (controls) on a pellet of modified insect diet (Gupta et al, 2005;Ruffner et al, 2013). To prevent injuries each larva was kept separately in 128-cell bioassay trays (Frontier Agricultural Sciences, Delaware, USA).…”

Section: Insect Assaysmentioning

confidence: 99%

Insect pathogenicity in plant-beneficial pseudomonads: phylogenetic distribution and comparative genomics

et al. 2016

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Bacteria of the genus Pseudomonas occupy diverse environments. The Pseudomonas fluorescens group is particularly well-known for its plant-beneficial properties including pathogen suppression. Recent observations that some strains of this group also cause lethal infections in insect larvae, however, point to a more versatile ecology of these bacteria. We show that 26 P. fluorescens group strains, isolated from three continents and covering three phylogenetically distinct sub-clades, exhibited different activities toward lepidopteran larvae, ranging from lethal to avirulent. All strains of sub-clade 1, which includes Pseudomonas chlororaphis and Pseudomonas protegens, were highly insecticidal regardless of their origin (animals, plants). Comparative genomics revealed that strains in this sub-clade possess specific traits allowing a switch between plant-and insect-associated lifestyles. We identified 90 genes unique to all highly insecticidal strains (sub-clade 1) and 117 genes common to all strains of sub-clade 1 and present in some moderately insecticidal strains of sub-clade 3. Mutational analysis of selected genes revealed the importance of chitinase C and phospholipase C in insect pathogenicity. The study provides insight into the genetic basis and phylogenetic distribution of traits defining insecticidal activity in plant-beneficial pseudomonads. Strains with potent dual activity against plant pathogens and herbivorous insects have great potential for use in integrated pest management for crops.

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Section: Insect Assaysmentioning

confidence: 99%

Insect pathogenicity in plant-beneficial pseudomonads: phylogenetic distribution and comparative genomics

et al. 2016

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“…The larvae were reared under a 16 h light: 8 h dark light regime at 27711C and 65% R.H. The composition of artificial diet on which the larvae were transferred and maintained throughout the experiment was similar to that described by Gupta et al (2005), and was composed of chickpea flour (C. arietinum L.) (56.0 g), kidney bean (Phaseolus vulgaris) (51.3 g) wheat germ, (Triticum sp) (26.0 g), agar (16.4 g), dried yeast powder (31.6 g), casein (15.20 g), ascorbic acid (3.2 g), formaldehyde 40% (2.0 ml), ABDEC drops (multivitamin solutions, 1.5 ml), multivitamin capsules (2), a-tacopherol (Evion 400 mg, 1 capsule), methyl-p-hydroxybenzoate (1.8 ml), and distilled water (820 ml).…”

Section: Insect Rearingmentioning

confidence: 99%

Bioinsecticidal activity of Murraya koenigii miraculin‐like protein against Helicoverpa armigera and Spodoptera litura

Gahloth¹,

Shukla²,

Birah³

et al. 2011

Arch Insect Biochem Physiol

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Miraculin-like proteins, belonging to the Kunitz superfamily, are natural plant defense agents against pests and predators, and therefore are potential biopesticides for incorporation into pest-resistant crops. Here, a miraculin-like protein from Murraya koenigii was assessed for its in vitro and in vivo effects against two polyphagous lepidopteran insect pests, Helicoverpa armigera and Spodoptera litura. M. koenigii miraculin-like protein (MKMLP) inhibited the trypsin-like activity and total protease activity of H. armigera gut proteinases (HGP) by 78.5 and 40%, respectively, and S.litura gut proteinases (SGP) by 81 and 48%, respectively. The inhibitor was stable and actively inhibited the proteolysis of both HGP and SGP enzymes for up to 72 h. Incorporation of MKMLP into artificial diet adversely affected the growth and development of pests in a dose-dependent manner. After 10 days of feeding on diets containing 200 µM MKMLP, larval weight was reduced to 69 and 44.8% and larval mortality was increased to 40 and 43.3% for H. armigera and S litura, respectively. The LC(50) of MKMLP was 0.34 and 0.22% of the diet for H.armigera and S. litura, respectively. These results demonstrate the efficacy of MKMLP as a potential plant defense agent against H. armigera and S. litura.

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“…Baculoviruses have also been recognized as efficient vectors that can be used for the production of numerous recombinant proteins (Contreras-Gomez et al, 2014;Mabashi-Asazuma et al, 2014). There have been many attempts to increase the efficiency of virus production, including the use of suitable containers (Sudheendrakumar et al, 2008), artificial diets (Chen et al, 2000;Gupta et al, 2005;Elvira et al, 2010a), specific inoculum dose and stage of inoculation (Narayanan & Jayaraj, 2002), and optimization of the rearing temperature and harvesting time of infected insects (Cherry et al, 1997;Subramanian et al, 2006). Baculoviruses replicate only in live insects or insect cultures, and producing them at an industrial scale is challenging.…”

Section: Introductionmentioning

confidence: 99%

Effect of juvenile hormone and pyriproxyfen treatments on the production ofSpodoptera lituranuclear polyhedrosis virus

Liao

Kuo

Shih

et al. 2016

Entomologia Exp Applicata

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Efficient production is essential for developing baculoviruses into marketable bioinsecticides. In this study, we evaluated how juvenile hormone (JH) III and the JH analog pyriproxyfen affected the production efficiency of Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) Nucleopolyhedrovirus (SpltNPV) in SpltNPV-inoculated early sixth instars. Both the JH III and pyriproxyfen treatments significantly enhanced the body size and weight of S. litura, which resulted in a 1.5-to 2-fold increase in the production of occlusion bodies (OBs). The OBs obtained from JH-and pyriproxyfen-treated larvae were as pathogenic as those produced in untreated larvae. More importantly, JH-or pyriproxy-fen-treated larvae consumed less than twice the amount of food consumed by two untreated larvae. These results indicate that JH and pyriproxyfen treatments improve the production efficiency of SpltNPV in S. litura. SpltNPV infection affected the development of S. litura not only as larvae, but also during subsequent growth stages and generations.

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Improved artificial diet for mass rearing of the tobacco caterpillar, Spodoptera litura (Lepidoptera: Noctuidae)

Cited by 56 publications

References 3 publications

Insect pathogenicity in plant-beneficial pseudomonads: phylogenetic distribution and comparative genomics

Insect pathogenicity in plant-beneficial pseudomonads: phylogenetic distribution and comparative genomics

Bioinsecticidal activity of Murraya koenigii miraculin‐like protein against Helicoverpa armigera and Spodoptera litura

Effect of juvenile hormone and pyriproxyfen treatments on the production ofSpodoptera lituranuclear polyhedrosis virus

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