Improved anticancer delivery of paclitaxel by albumin surface modification of PLGA nanoparticles

Abstract

BackgroundNanoparticles (NPs) play an important role in anticancer delivery systems. Surface modified NPs with hydrophilic polymers such as human serum albumin (HSA) have long half-life in the blood circulation system.MethodsThe method of modified nanoprecipitation was utilized for encapsulation of paclitaxel (PTX) in poly (lactic-co-glycolic acid) (PLGA). Para-maleimide benzoic hydrazide was conjugated to PLGA for the surface modifications of PLGA NPs, and then HSA was attached on the surface of prepared NPs by maleimide attachment to thiol groups (cysteines) of albumin. The application of HSA provides for the longer blood circulation of stealth NPs due to their escape from reticuloendothelial system (RES). Then the physicochemical properties of NPs like surface morphology, size, zeta potential, and in-vitro drug release were analyzed.ResultsThe particle size of NPs ranged from 170 to 190 nm and increased about 20–30 nm after HSA conjugation. The zeta potential was about -6 mV and it decreased further after HSA conjugation. The HSA conjugation in prepared NPs was proved by Fourier transform infrared (FT-IR) spectroscopy, faster degradation of HSA in Differential scanning calorimetry (DSC) characterization, and other evidences such as the increasing in size and the decreasing in zeta potential. The PTX released in a biphasic mode for all colloidal suspensions. A sustained release profile for approximately 33 days was detected after a burst effect of the loaded drug. The in vitro cytotoxicity evaluation also indicated that the HSA NPs are more cytotoxic than plain NPs.ConclusionsHSA decoration of PLGA NPs may be a suitable method for longer blood circulation of NPs.

Ferritin Decorated PLGA/Paclitaxel Loaded Nanoparticles Endowed with an Enhanced Toxicity Toward MCF-7 Breast Tumor Cells

“…In agreement with that previously reported by Esfandyari-Manesh and coworkers 7 , we found that the toxicity, herein evaluated by MTT assay, of PLGA coated with BSA and loaded with PTX (PLGA-PTX-BSA) is significantly higher than the one observed for the commercially available Paclitaxel Kabi and for the PTX loaded PLGA-PTX-CTRL. The observed behavior may be accounted for the presence of gp60 (albondin) receptor and caveolar transport as both systems are involved in the transendothelial cell transport of albumin 7 . The data reported in Figure 5 show that the cytotoxicity is further enhanced by using PLGA coated with Figure S4) and it was less than 10%…”

Magnetic responsive of paclitaxel delivery system based on SPION and palmitoyl chitosan

“…It is apparent from Fig.5(c) that the mean diameters of the nanoparticles increased slightly after drug loading. Although the dimension of nanoparticles was expected to show a dramatic increase after drug loading [44][45][46], their size is pale in comparison to their size before encapsulating PTX (p>0.05). Moreover, in some samples, gradual reduction in the size of drug-loaded nanoparticles was observed.…”

From Lab to Pharmacy Shelves: The Story of a Plant Derived Anticancer Drug, "Paclitaxel"

“…The studies on Paclitaxel are still ongoing and researchers are trying to make it even more efficient. Recent studies show the efficacy of nanoparticle bound paclitaxel (Sparreboom et al, 2005;Jia et al, 2015 andEsfandyari-Manesh et al, 2015). Some studies are focused on finding a way to decrease paclitaxel associated adverse effects, such as the use of adjuvants for targeted drug delivery.…”