Improved angiogenesis and healing in crush syndrome by fibroblast growth factor-2–containing low-molecular-weight heparin (Fragmin)/protamine nanoparticles

Takikawa, Makoto; Nakamura, Shingo; Takabayashi, Yuki; Fujita, Masanori; Hattori, Hidemi; Kubota, Toshihiro; Ishihara, Miya

doi:10.1016/j.jss.2015.03.022

Cited by 16 publications

(11 citation statements)

References 25 publications

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“…The D/P NPs were prepared by modifying the method as described earlier. 20 , 25 To summarize, 2.5 mL of dalteparin solution (Fragmin: 6.4 mg/mL; Kissei Pharmaceutical Co., Tokyo, Japan), followed by 1.2 mL of protamine solution (10 mg/mL; Mochida Pharmaceutical Co., Tokyo, Japan) was added into 500 mL of saline (Otsuka Normal Saline; Otsuka Pharmaceutical Co., Ltd. Tokyo, Japan). The white solution was mixed vigorously.…”

Section: Methodsmentioning

confidence: 99%

Enhanced effect of fibroblast growth factor-2-containing dalteparin/protamine nanoparticles on hair growth

Takabayashi¹,

Nambu²,

Kuwabara³

et al. 2016

CCID

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PurposeAlthough treatments for alopecia are in high demand, not all treatments are safe and reliable. Dalteparin/protamine nanoparticles (D/P NPs) can effectively carry growth factors (GFs) such as fibroblast GF (FGF)-2. The purpose of this study was to identify the effects of FGF-2-containing D/P NPs (FGF-2&D/P NPs) on hair growth.Patients and methodsIn this study, the participants were 12 volunteers with thin hair. One milliliter of FGF-2 (100 ng/mL) and D/P NPs (56 μg/mL) was applied and massaged on the skin of the scalp by the participants twice a day. They were evaluated for 6 months. Participants were photographed using a digital camera for general observation and a hair diagnosis system for measuring hair diameter.ResultsThe mean diameter of the hairs was significantly higher following the application of FGF-2&D/P NPs for 6 months. Objective improvements in thin hair were observed in two cases. Nine participants experienced greater bounce and hair resilience.ConclusionThe transdermal application of FGF-2&D/P NPs to the scalp can be used as a new treatment for alopecia.

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Section: Methodsmentioning

confidence: 99%

Enhanced effect of fibroblast growth factor-2-containing dalteparin/protamine nanoparticles on hair growth

Takabayashi¹,

Nambu²,

Kuwabara³

et al. 2016

CCID

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“…Neutralizing antibodies against FGF2 suppressed the restorative angiogenesis in a wounded chicken chorioallantoic membrane [ 143 ]. In a related context, recombinant FGF2 and FGF1 were demonstrated to efficiently stimulate angiogenesis in rodent models of myocardium ischemia [ 61 ], hindlimb ischemia [ 62 , 63 , 64 ], muscle damage [ 65 ], bone repair [ 66 , 67 ], ear ulcer [ 68 ], and de-vascularized sternum repair [ 69 ].…”

Section: Cell Processes Underlying the Stimulation Of Tissue Repair By Fgfmentioning

confidence: 99%

Cellular Mechanisms of FGF-Stimulated Tissue Repair

Prudovsky

2021

Cells

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Growth factors belonging to the FGF family play important roles in tissue and organ repair after trauma. In this review, I discuss the regulation by FGFs of the aspects of cellular behavior important for reparative processes. In particular, I focus on the FGF-dependent regulation of cell proliferation, cell stemness, de-differentiation, inflammation, angiogenesis, cell senescence, cell death, and the production of proteases. In addition, I review the available literature on the enhancement of FGF expression and secretion in damaged tissues resulting in the increased FGF supply required for tissue repair.

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“…In this study, we demonstrated that the NPs-modified CAG scaffold with cytokines loaded almost covers these key elements of tissue engineering. Nanoparticles constructed with heparin have been studied as cytokine delivery carriers for years because many cytokines, such as stromal SDF-1, BMP-2, vascular endothelial growth factor, placental growth factor, platelet-derived growth factor, and fibroblast growth factor, [29][30][31][32][33] can bind to heparin through their conserved amino acid sequences. The use of chitosan and heparin for the delivery of bioactive molecules has many advantages, including being inexpensive, biodegradable, noncytotoxic, nonimmunogenic, 34,35 easy to process, absorbable, and having safe degradation end product.…”

Section: Discussionmentioning

confidence: 99%

Nanoparticle-modified chitosan-agarose-gelatin scaffold for sustained release of SDF-1 and BMP-2

Wang

Guo

Chen

et al. 2018

IJN

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BackgroundStromal cell-derived factor 1 (SDF-1) is an important chemokine for stem cell mobilization, and plays a critical role in mobilization of mesenchymal stem cells (MSCs). Bone morphogenetic protein 2 (BMP-2) plays a critical role in osteogenesis of MSCs. However, the use of SDF-1 and BMP-2 in bone tissue engineering is limited by their short half-lives and rapid degradation in vitro and in vivo.MethodsThe chitosan oligosaccharide/heparin nanoparticles (CSO/H NPs) were first prepared via self-assembly. Chitosan-agarose-gelatin (CAG) Scaffolds were then synthesized via gelation technology using cross-linked chitosan, agarose, and gelatin, and were modified by CSO/H NPs. The encapsulation efficiency and release kinetics of SDF-1 and BMP-2 were quantified using an enzyme-linked immunosorbent assay. A CCK-8 assays were used to evaluate biocompatibility of NP-modified scaffolds. The biological activity of the loaded SDF-1 and BMP-2 was evaluated using the transwell migration assay and osteogenic induction assay. An animal MSC recruitment model was used to study the ability of SDF-1 released from NP-modified scaffolds to induce migration of MSCs.ResultsIn this study, we developed a novel nanoparticle-modified CAG scaffold for the delivery of SDF-1 and BMP-2. CCK-8 assays demonstrated excellent biocompatibility of NP-modified scaffolds. In addition, we investigated the release of SDF-1 and BMP-2 from NP-modified scaffolds, and evaluated the effect of released SDF-1 on MSC migration. The effect of released BMP-2 on MSC osteogenesis was also examined. In vitro cell migration assays showed that SDF-1 released from NP-modified scaffolds retained its migration activity; osteogenesis studies demonstrated that released BMP-2 exhibited a strong ability to induce differentiation towards osteoblasts. Our in vivo recruitment assays showed continuous chemotactic response of MSCs to SDF-1 released from the NP-modified scaffold.ConclusionThe simplicity of synthesizing CSO/H NP-modified CAG scaffolds, combined with its high cytokine loading capacity and sustained release effect, renders NP-modified CAG scaffold an attractive candidate for sustained release of SDF-1 and BMP-2 to promote bone repair and regeneration.

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Improved angiogenesis and healing in crush syndrome by fibroblast growth factor-2–containing low-molecular-weight heparin (Fragmin)/protamine nanoparticles

Cited by 16 publications

References 25 publications

Enhanced effect of fibroblast growth factor-2-containing dalteparin/protamine nanoparticles on hair growth

Enhanced effect of fibroblast growth factor-2-containing dalteparin/protamine nanoparticles on hair growth

Cellular Mechanisms of FGF-Stimulated Tissue Repair

Nanoparticle-modified chitosan-agarose-gelatin scaffold for sustained release of SDF-1 and BMP-2

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