Improved aeroponic culture of inocula of arbuscular mycorrhizal fungi

Mohammad, Asif; Khan, Asif; Kuek, Clem

doi:10.1007/s005720050278

Cited by 51 publications

(17 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several different methods were employed to propagate AMF such as single or monosporic (Fracchia et al, 2001;Selvakumar et al, 2016), hairy root (de Souza and Declerck 2003), solid substrate (Millner and Kitt 1992;Douds et al, 2010), aeroponic (Mohammad et al, 2000), and hydroponic (Tajini et al, 2009). However, storing the propagated AMF spore requires technical skills and preliminary knowledge, thus making it difficult for farmers.…”

Section: Effect Of Mycorrhizal Inoculation On the Growth Parametersmentioning

confidence: 99%

Trap Culture Technique for Propagation of Arbuscular Mycorrhizal Fungi using Different Host Plants

Selvakumar¹,

Kim²,

Walitang³

et al. 2016

Korean Journal of Soil Science and Fertilizer

View full text Add to dashboard Cite

Arbuscular mycorrhizal fungi (AMF) spore propagation and long term maintenance is still a complicated technique for farmers. The use of AMF for their ability to promote plant growth and protect plants against pathogen attack and environmental stresses demands AMF propagation for large scale application. This study aimed to propagate AMF spores by trap culture technique and assess their ability to propagate with different host plants in a continuous plant cycle. Mycorrhizal inoculation by trap culture in maize resulted in longer shoots and roots than sudangrass plants. Increase in dry weight with higher percentage also was observed for maize plants. After first and second plant cycle, maize plants had the higher percentage of mycorrhizal response in terms of colonization and arbuscules than sudangrass. Maximum in spore count also achieved in the pots of maize plants. The results show that maize plant is more suitable host plant for AMF spore propagation and trap culture technique can be used effectively to maintain the AMF culture for long time.

show abstract

Section: Effect Of Mycorrhizal Inoculation On the Growth Parametersmentioning

confidence: 99%

Trap Culture Technique for Propagation of Arbuscular Mycorrhizal Fungi using Different Host Plants

Selvakumar¹,

Kim²,

Walitang³

et al. 2016

Korean Journal of Soil Science and Fertilizer

View full text Add to dashboard Cite

show abstract

“…In an interesting experiment, Jarstfer and Sylvia (1995) tested aeroponic devises, atomizing disk, pressurized spray through a microirrigated nozzle, and an ultrasonically generated fog of nutrient solution with droplets and concluded that pump and nozzle spray systems were the most tailored systems for mass production of AM fungi. Similarly, Mohammad et al (2000) compared the atomizing disk with the ultrasonic nebulizer technology and found that the ultrasonic nebulizer method was the finest method for the mass production of AM fungi. The finding of the earlier researchers for the mass production of AM fungi using substrate free cultivation systems has been summarized in the tabular form (Table 2).…”

Section: Substrate Free Production Systemmentioning

confidence: 99%

Mass Production Techniques of Arbuscular Mycorrhizal Fungi: Major Advantages and Disadvantages: A Review

Akhtar¹,

Abdullah²

2014

Biosci., Biotechnol. Res. Asia

View full text Add to dashboard Cite

Numerous techniques have been developed in the past few decades for the mass production of arbuscular mycorrhizal (AM) fungi. The main obstacle behind the mass production techniques of AM fungi is the obligate nature of this biotrophic fungus and species level identification of AM fungal species could not be possible at the early stage of development. Currently, in-vitro cultivation methods such as hydroponic system and root organ culture has been widely used for the mass production of AM fungi. These methods are not only maintain the quality of AM fungal propagules but they can also be developed as cost effective methods for the mass propagation of AM fungi. The aim of this paper is to highlight the recent and advanced methods used for the mass production of AM fungi.

show abstract

“…As gramíneas são as mais utilizadas, e são cultivadas em substratos ou misturas de substratos -areia, solo, vermiculita, turfa, entre outros - (Gianinazzi & Vosátka, 2004). Outros sistemas de produção de inoculante utilizados são o aeropônico (Mohammad et al, 2000) e o hidropônico, que permitem a produção de raízes colonizadas e de esporos. Técnicas mais recentes têm possibilitado a produção de inóculo a partir de raízes transformadas para a obtenção de cultura monospórica de FMA in vitro (Schubert & Lubraco, 2000;Declerck et al, 2005).…”

Section: Introductionunclassified

Aplicação de formononetina na colonização e esporulação de fungos micorrízicos em braquiária

Novais

Siqueira

2009

Pesq. agropec. bras.

View full text Add to dashboard Cite

Resumo -O objetivo deste trabalho foi avaliar o efeito de aplicações de Mycoform na colonização micorrízica e esporulação de 13 isolados de fungos micorrízicos arbusculares em Brachiaria decumbens. O experimento foi conduzido em casa de vegetação, em solo esterilizado, com delineamento experimental inteiramente casualizado, em arranjo fatorial 3x13, com cinco repetições. O produto foi aplicado no plantio e foi ou não aplicado uma segunda vez 60 dias depois, na quantidade de 2 mg kg -1 de solo. Aos 150 dias de crescimento das plantas, foram coletadas amostras de raízes e de solo rizosférico, para a avaliação de colonização radicular e densidade de esporos. Houve estímulo do Mycoform nos parâmetros avaliados, efeito que variou com os isolados estudados. Foi observado efeito significativo da aplicação do Mycoform na colonização das raízes pelos isolados Glomus clarum DCS 09 e DCS 10, Paraglomus occultum DCS 06 e Acaulospora delicata DCS 02 e na esporulação dos isolados G. clarum DCS 09 e DCS 10, P. occultum DCS 06 e DCS 31, Glomus etunicatum DCS 12, A. delicata DCS 30 e Kuklospora colombiana DCS 03. O incremento na esporulação atingiu 89% e, na colonização, 60%, o que confirma os benefícios da formononetina na colonização e na esporulação dos fungos micorrízicos arbusculares.Termos para indexação: Brachiaria decumbens, estimulante de micorriza, multiplicação de FMAs, Mycoform, simbiose radicular. Formononetin application on colonization and sporulation of arbuscular mycorrhizal fungi in BrachiariaAbstract -The aim of this work was to evaluate the effects of Mycoform on both sporulation and colonization of 13 arbuscular mycorrhiza fungi (AMF) isolates in Brachiaria decumbens. The experiment was carried out in greenhouse conditions with sterile soil, in a completely randomized design with 3x13 factorial treatments and five repetitions. The product was applied once at planting and was or was not applied a second time 60 days afterwards, at an amount of 2 mg kg -1 of soil. Plants were allowed to grow for 150 days, when root and rhizospheric soil samples were collected to evaluate the percentage of colonized root segments and the spore density. Mycoform effects were different among the fungal isolates. It had significant effect on colonization for the isolates Glomus clarum DCS 09 and DCS 10, Paraglomus occultum DCS 06 and Acaulospora delicata DCS 02, and on spore density for the isolates G. clarum DCS 09 and DCS 10, P. occultum DCS 06 and DCS 31, Glomus etunicatum DCS 12, A. delicata DCS 30 and Kuklospora colombiana DCS 03. Maximum increases due to Mycoform were 60% for colonization and 89% for spore density. These results confirm formononetin effects on AMF colonization and on AMF sporulation.Index terms: Brachiaria decumbens, mycorrhizal stimulate, AMF multiplication, Mycoform, root symbiosis. IntroduçãoA produção agrícola sustentável está associada ao desenvolvimento de tecnologias que minimizem os impactos ambientais e que poupem insumos não renováveis, como os fertilizantes a base de fósforo. Nesse sentido, há gra...

show abstract

Improved aeroponic culture of inocula of arbuscular mycorrhizal fungi

Cited by 51 publications

References 12 publications

Trap Culture Technique for Propagation of Arbuscular Mycorrhizal Fungi using Different Host Plants

Trap Culture Technique for Propagation of Arbuscular Mycorrhizal Fungi using Different Host Plants

Mass Production Techniques of Arbuscular Mycorrhizal Fungi: Major Advantages and Disadvantages: A Review

Aplicação de formononetina na colonização e esporulação de fungos micorrízicos em braquiária

Contact Info

Product

Resources

About