Improved activity and stability of Rhizopus oryzae lipase via immobilization for citronellol ester synthesis in supercritical carbon dioxide

Synthesis of eugenyl acetate by enzymatic reactions in supercritical carbon dioxide

“…(4). The quality of this adjustment was evaluated from the determination coefficient (R 2 ) using the MATLAB software (2014Ra, MathWorks, Natick, MA, USA)…”

“…Therefore, biocatalyzed chemical synthesis becomes of great interest due to the high chemo-, regio-and stereo-selectivity of the enzymes, which provide possible in vitro synthesis of naturally existing single enantiomers of specific compounds [2]. Moreover, the esters can be considered as natural [2][3][4], since the process meets the required conditions by the legislation. For example, the substrates or raw materials used in process are natural and only with integrated downstream separation of products and unreacted substrates [11].…”

Biosynthesis of Iminodiacetic Acid from Iminodiacetonitrile by Immobilized Recombinant <i>Escherichia coli</i> Harboring Nitrilase

“…Among these, entrapment is the most commonly used method which is cost-effective, easy to handle and manipulate [Shen et al, 2009]. In addition, gel materials are not only mild but also soluble, biodegradable and abrasive, and cells functioned especially well in these supports [Dhake et al, 2011]. Synthetic gels, on the contrary, have better mechanical properties, but mostly lower substrates and product diffusion coefficients [Leenen et al, 1996].…”

“…To date, there have been few reports about immobilization of nitrilase-producing bacteria employing chitosan , agar [Nigam et al, 2009], alginate [Chen et al, 2008;Shen et al, 2009], silica [Swartz et al, 2009], silica-carrageenan [Kabaivanova et al, 2011], glutaraldehyde [He et al, 2010], polyacrylamide and polyvinyl alcohol (PVA) [Vejvoda et al, 2006] as matrices. Though the gel beads of photo-crosslinked PVA and calcium-alginate mixtures have been applied successfully as carriers in a denitrification process [Dhake et al, 2011;Wang et al, 2007], there are no studies about using multiple polymers for immobilization of recombinant cells harboring nitrilase [Bryaskova et al, 2010]. So far, there have been no reports about immobilized recombinant nitrilase capable of producing IDA.…”

Utilization of fish meal and fish oil for production of Cryptococcus sp. MTCC 5455 lipase and hydrolysis of polyurethane thereof

“…3.1.1.3) are an important group of biotechnologically relevant enzymes as they catalyze several reactions: hydrolysis, esterification, alcoholysis, aminolysis, peroxidations, epoxidations and interesterification (Contesini et al 2010). The apparent promiscuity of these enzymes make lipases useful in numerous industrial processes, such as the synthesis of food ingredients (Dhake et al 2011), their use as additives to detergents (Thirunavukarasu et al 2008) and to obtain enantiopure drugs (Lou et al 2006) and other refined products. Among microbial lipases, yeast lipases have received less attention and few reports are available on lipase producing yeasts such as Yarrowia lipolytica (Pignede et al 2000), Candida antarctica (Lou et al 2006) and C. rugosa (Herbst et al 2012) and only limited research has been directed towards the lipase of Cryptococcus sp.…”