Improved acetate peel technique

Lane, D. W. J.

doi:10.2110/jsr.32.870

Cited by 7 publications

(5 citation statements)

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“…[ 10 12 ] Few disadvantages however remain like acetate films are susceptible to contamination and have a tendency to curl up. [ 11 12 ] Minerals cannot be identified by optical properties like birefringence with peels (inability to visualize optical properties of crystals). [ 6 ]…”

Section: Discussionmentioning

confidence: 99%

“…[ 3 9 10 ] Peel technique involves dis-embedding a layer of any tissue by means of removal of the mineral matrix with acids and re-embedding the exposed cellular structures in clear acetate peel. [ 3 9 11 ] Studies have shown the efficacy of the said acetate method in yielding a large number of sequential replicas from an undecalcified tooth in a time saving and economical manner. [ 10 ] However, its role in reproducing structural details of hard tissues of a tooth is less explored.…”

Section: Introductionmentioning

confidence: 99%

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Peels as an alternative to ground sections – An in vitro microscopic study

Rath

Raghunath

2021

J Oral Maxillofac Pathol

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Background: The routine approach to delve into the organization of mineralized and nonmineralized structures of teeth is by studying whole tooth or slices of it by making thin section which requires laborious grinding or employing specialized equipment and also leads to specimen wastage. Peels hitherto utilized for fossil studies hold promise in overcoming the aforesaid shortcomings. Although the acetate peel technique has been modified for the study of tooth structure, the field remains largely unexplored. Aim: The current study was taken up to explore the usefulness of cellulose acetate peels in reproducing microscopic structures of teeth as seen in routine ground sections and further if they could supplement or replace the same. Materials and Methods: Extracted human teeth were embedded in plaster blocks in longitudinal and transverse orientation, ground and polished with silicon carbide paper. Following etching, washing and drying, the polished surface was wet with acetone and precut cellulose acetate film was placed over it and allowed to dry. As the acetate polymer dissolved in acetone and subsequently re-polymerized after setting into the micro reliefs produced by tooth etching, it registered the microscopic tooth details on its surface. The peels were mounted and secured on a glass slide and subjected to routine light and phase contrast microscopy for observing captured details of the tooth structure. Results and Conclusion: Acetate peels successfully reproduced most of the microscopic tooth details which were better than those observed in ground tooth sections. Hence, this technique could be considered as a quick, durable and inexpensive alternative or supplement to routine thin ground sections of dental hard tissues.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Peels as an alternative to ground sections – An in vitro microscopic study

Rath

Raghunath

2021

J Oral Maxillofac Pathol

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“…The peel technique was described by Walton (1928), who used films from a cellulose ester solution. Since then it has been improved by several authors (e.g., Joy et al 1956;Lane 1962;Katz and Friedman 1965). The peel method was also used to elaborate precise three-dimensional reconstructions (e.g., Baker 1978).…”

Section: Data Capturementioning

confidence: 99%

3D morphometry of polyconitid rudist bivalves based on grinding tomography

Pascual-Cebrian

Hennhöfer

Götz

2012

Facies

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Previous taxonomic studies have shown that polyconitid rudists have a characteristic arrangement of the myocardinal system with an ectomyophoral cavity on the posterior side of the left valve. The specific arrangement of the myophores and associated cavities defines the different genera. However, there has been little research on the three-dimensional spatial distribution and size of the internal features, for want of a technique that is suitable for large and low density-contrast specimens. The tomographic technique described herein is based on automatic serial grinding and serial scanning; the resulting images are treated with biomedical image software. The technique has been applied to a pair of well-preserved specimens of Polyconites verneuili from the Upper Aptian of Spain. Fifteen quantitative characters have been obtained using multiplanar virtual cuts, volume-rendering, and isosurfaces reconstructions. The study revealed the shape, size, and distribution of the ectomyophoral, body and accessory cavities, the lengths and volumes of the teeth, and the arrangement of the myophores. We conclude that this technique facilitates the description of rudist bivalves and is suitable for other fossils; moreover, it has the potential to be used in other fields of geology.

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“…Acetate peels have been used in petrology and palaeontology for some time to record the structure and fabric of polished sections of specimens (Walton, 1928;Butler, 1935;Darrah, 1936;Easton, 1942;Beales, 1960;Lane, 1962;Overlau, 1963). Only recently has the technique been applied to polished blocks and thin sections of soils by Jenkins (1970) and Conway and Jenkins (1978).…”

Section: Ancillary Techniques 307mentioning

confidence: 99%

Micromorphology of Soils

Fitzpatrick

1984

150

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Improved acetate peel technique

Cited by 7 publications

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Peels as an alternative to ground sections – An in vitro microscopic study

Peels as an alternative to ground sections – An in vitro microscopic study

3D morphometry of polyconitid rudist bivalves based on grinding tomography

Micromorphology of Soils

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