Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism

Jiang, Yun-Gang; Wang, Chuyan; Jin, Chao; Jia, Jizeng; Guo, Xingchen; Zhang, Guo-Zheng; Gui, Zhongzheng

doi:10.1590/s1517-83822014000200048

Cited by 30 publications

(16 citation statements)

References 24 publications

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“…DNJ can be isolated from mulberry ( Jiang et al, 2014 ; Wang et al, 2014 , 2015 ; E et al, 2017 ; Hu et al, 2017 ) or produced by Bacillus species ( Lee et al, 2013 ; Seo et al, 2013 ; Do et al, 2015 ; Cai et al, 2017 ). DNJ may be a potential drug for treating post-prandial hyperglycemia with few side effects ( Asai et al, 2011 ; Jiang et al, 2014 ). With the study on DNJ, it has been found to have many health promoting properties.…”

Section: Introductionmentioning

confidence: 99%

1-Deoxynojirimycin (DNJ) Ameliorates Indomethacin-Induced Gastric Ulcer in Mice by Affecting NF-kappaB Signaling Pathway

Piao¹,

Sui

et al. 2018

Front. Pharmacol.

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Gastric ulcer (GU) is a main threat to public health. 1-Deoxynojirimycin (DNJ) has antioxidant and anti-inflammatory properties and may prevent GU but related mechanism remains unclear. DNJ was extracted from the supernatants of Bacillus subtilis by using ethanol and purified by using CM-Sepharose chromatography. A GU mouse model was induced by indomethacin. The functional role of DNJ in GU mice was explored by measuring the main molecules in the NF-KappaB pathway. After the model establishment, 40 GU mice were evenly assigned into five categories: IG (received vehicle control), LG (10 μg DNJ daily), MG (20 μg DNJ daily), HG (40 μg DNJ daily), and RG (0.5 mg ranitidine daily). Meanwhile, eight healthy mice were assigned as a control group (CG). After 1-month therapy, weight and gastric volume were investigated. The levels of serum inflammatory cytokines (IL-6 and TNF-α), antioxidant indices [superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH)], and oxidant biomarker malondialdehyde (MDA) were examined via ELISA. Meanwhile, inflammatory cytokine (IL-6 and TNF-α) levels, and key molecules (NF-κB p65), cyclooxygenase 1 (COX-1 and COX2) involved in NF-κB pathway, were analyzed by using Western Blot. COX-1 and COX-2 levels were further measured by immunohistochemistry. The effects of DNJ on gastric functions were explored by measuring the changes of Motilin (MOT), Substance P (SP), Somatostatin (SS), and Vasoactive intestinal peptide (VIP) in GU mouse models with ELISA Kits. The results indicated that DNJ prevented indomethacin-caused increase of gastric volume. DNJ improved histopathology of GU mice when compared with the mice from IG group (P < 0.05). DNJ consumption decreased the levels of IL-6 and TNF-α (P < 0.05). DNJ increased antioxidant indices of GU mice by improving the activities of SOD, CAT and reduced GSH, and reduced MDA levels (P < 0.05). DNJ increased the levels of prostaglandin E2, COX-1, COX2, and reduced the levels of and NF-κB p65 (P < 0.05). DNJ showed protection for gastric functions of GU mice by reducing the levels of MOT and SP, and increasing the levels of SS and VIP. DNJ treatment inactivates NF-κB signaling pathway, and increases anti-ulceration ability of the models.

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Section: Introductionmentioning

confidence: 99%

1-Deoxynojirimycin (DNJ) Ameliorates Indomethacin-Induced Gastric Ulcer in Mice by Affecting NF-kappaB Signaling Pathway

Piao¹,

Sui

et al. 2018

Front. Pharmacol.

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“…Studies have also reported the effectiveness of mulberry leaves in skin aging [49,50] and neurodegenerative disorders including Alzheimer’s disease and Parkinson’s disease [51]. Moreover, sedative effect of mulberry fruits and anti-inflammatory, diuretic, antitussive, and antipyretic properties of mulberry root bark have been studies [52]. In addition to DNJ, many other bioactive compounds including flavonoids, alkaloids, steroids, and coumarins also exist in mulberry leaves [53].…”

Section: 1-deoxynojirimycinmentioning

confidence: 99%

“…The recovery and purity of DNJ in the end product were >90% and >15%, respectively [62]. Jiang et al stated that fermentation of mulberry leaf by Ganoderma lucidum produced the highest DNJ content [52]. The optimal condition for mulberry fermentation, obtained from RSM, was the following: pH 6.97, potassium nitrate content 0.81%, and inoculums volume 2 mL.…”

Section: 1-deoxynojirimycinmentioning

confidence: 99%

1-Deoxynojirimycin: Occurrence, Extraction, Chemistry, Oral Pharmacokinetics, Biological Activities and In Silico Target Fishing

et al. 2016

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1-Deoxynojirimycin (DNJ, C 6 H 13 NO 4 , 163.17 g/mol), an alkaloid azasugar or iminosugar, is a biologically active natural compound that exists in mulberry leaves and Commelina communis (dayflower) as well as from several bacterial strains such as Bacillus and Streptomyces species. Deoxynojirimycin possesses antihyperglycemic, anti-obesity, and antiviral features. Therefore, the aim of this detailed review article is to summarize the existing knowledge on occurrence, extraction, purification, determination, chemistry, and bioactivities of DNJ, so that researchers may use it to explore future perspectives of research on DNJ. Moreover, possible molecular targets of DNJ will also be investigated using suitable in silico approach.

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“…1-Deoxynojirimycin (DNJ), a natural alkaloid from plant mulberry or microorganism, is a conventional hypoglycemic drug in clinical application for effectively inhibiting α -glucosidase activity [ 7 , 8 ]. Thus, it could potently inhibit the metabolism of saccharides and lower blood glucose levels.…”

Section: Introductionmentioning

confidence: 99%

Improvement Activity of 1-Deoxynojirimycin in the Growth of Dairy Goat Primary Mammary Epithelial Cell through Upregulating LEF-1 Expression

Liu

Zhang

et al. 2018

BioMed Research International

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LEF-1/wnt10b is one of the most important signaling pathways regulating mammary gland growth and development and is also a potential target for molecular breeding. In this work, 1-deoxynojirimycin (DNJ), a natural alkaloid extracted from plant mulberry or microorganism, was found to have a positive activity in primary breast epithelial cell growth of dairy goats. The findings showed that, compared to the control, 6 μM DNJ in the DMEM/F12 medium in vitro greatly improved the density of dairy goat breast epithelial cell and significantly increased the LEF-1 mRNA level (P < 0.01) and thus enhanced cell growth. In addition, DNJ displayed a similar function in alleviating the growth suppression of epithelial cell and the decrease of LEF-1 mRNA level resulting from lentiviral-mediated LEF-1 knockdown. Simultaneously, no significant change of the mRNA levels of IGF-1 and Fgf10, the other two key regulators in mammary gland growth and development, could be detected. Furthermore, the mammary duct of DNJ-fed mouse illustrated a better development accompanied with a higher LET-1 mRNA level than that of the control. In conclusion, DNJ could improve breast epithelial cell growth through upregulating LEF-1 expression, which supplied a new means in studying mammary gland growth and development.

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Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism

Cited by 30 publications

References 24 publications

1-Deoxynojirimycin (DNJ) Ameliorates Indomethacin-Induced Gastric Ulcer in Mice by Affecting NF-kappaB Signaling Pathway

1-Deoxynojirimycin (DNJ) Ameliorates Indomethacin-Induced Gastric Ulcer in Mice by Affecting NF-kappaB Signaling Pathway

1-Deoxynojirimycin: Occurrence, Extraction, Chemistry, Oral Pharmacokinetics, Biological Activities and In Silico Target Fishing

Improvement Activity of 1-Deoxynojirimycin in the Growth of Dairy Goat Primary Mammary Epithelial Cell through Upregulating LEF-1 Expression

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