Improve the viability and extracellular polymeric substances bioactivity of Lactiplantibacillus plantarum VAL6 using the environmental adaptation

Nguyen, Phu-Tho; Nguyen, Tina; Nguyen, T.D.; Hoang, Quoc-Khanh; Nguyen, Huy-Viet

doi:10.1016/j.fbp.2021.11.006

Cited by 8 publications

(4 citation statements)

References 45 publications

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“…On the other hand, the pre-exposition to sublethal conditions SUB_2 (pH 5, NaCl 2%, 25 °C, for 48 h) reduced the lag phase starting from 4% NaCl ≥ 0.5 M. The ability of this species to face the osmotic stress was attributed to its capability to produce extracellular polymeric substances such as exopolysaccharides, which form a layer that protects the cells from water loss increasing the cell resistance to osmosis. 51,52 The exposition of L. plantarum to NaCl-induced osmotic stress can improve the autoaggregation and the adhesion as well as the pathogen adhesion inhibition, thus strengthening the probiotic. 53…”

Section: Discussionmentioning

confidence: 99%

Application of pre-adaptation strategies to improve the growth of probiotic lactobacilli under food-relevant stressful conditions

2023

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Section: Discussionmentioning

confidence: 99%

Application of pre-adaptation strategies to improve the growth of probiotic lactobacilli under food-relevant stressful conditions

2023

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“…In addition, membrane lipid oxidation by drying also damages the cellular membrane structure of bacteria (Teixeira et al, 1996). The synthesized EPSs with high antioxidant activity could effectively inhibit membrane oxidation leading to increased bacterial viability (Nguyen et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Efficacy of the incorporation between self-encapsulation and cryoprotectants on improving the freeze-dried survival of probiotic bacteria

Nguyen

et al. 2022

Journal of Applied Microbiology

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Aims This study aimed to improve the viability of probiotic bacteria during freeze‐drying by the combination of self‐encapsulation and cryoprotectants. Methods and Results Lactiplantibacillus plantarum VAL6 and Lactobacillus acidophilus VAR1 were exposed to environmental stresses including temperature, pH and increased CO2 concentration before performing freeze‐drying with the addition of cryoprotectants. The results proved that tested stresses can stimulate the bacteria to synthesize more extracellular polymeric substances to form self‐encapsulation that increases their freeze‐dried viability. In combination with cryoprotectants to form double‐layered microencapsulation, L. plantarum VAL6 stressed at pH 3.5 in combination with whey protein isolate could achieve the highest Improving Cell Viability of 4361‐fold, while L. acidophilus VAR1 stressed at 25oC in combination with alginate gave a maximum Improving Cell Viability of 73.33‐fold. Conclusions The combination of self‐encapsulation and cryoprotectants significantly improves the freeze‐dried viability of probiotics. Significance and Impact of the Study This is the first report that uses environmental stress to stimulate extracellular polymeric substance synthesis for self‐encapsulation formation combined with the addition of cryoprotectants to enhance the freeze‐dried survival of probiotics. This could be a novel approach in improving the viability of probiotic strains for various applications.

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“…Lactobacillus in a certain environment usually survive in the form of biofilm, 9 the extracellular polymers mainly refers to the bacterial secretion of a variety of biomolecular materials, such as extracellular polysaccharides (EPS) and proteins, nucleic acids, and so on 10,11 . The formation of Lactobacillus biofilm is significantly influenced by quorum sensing (QS) 12 .…”

Section: Introductionmentioning

confidence: 99%

“…8 Lactobacillus in a certain environment usually survive in the form of biofilm, 9 the extracellular polymers mainly refers to the bacterial secretion of a variety of biomolecular materials, such as extracellular polysaccharides (EPS) and proteins, nucleic acids, and so on. 10,11 The formation of Lactobacillus biofilm is significantly influenced by quorum sensing (QS). 12 Existing studies have shown that there are two systems that regulate biofilm forming ability through QS, LuxS/AI-2 type, and autoinducing signaling peptides (AIP) type, where Lactobacillus regulates strain activity through interspecies communication signal molecule AI-2 (Auto inducer, AI) and intraspecies regulation signal molecule AIP.…”

mentioning

confidence: 99%

Regulation and mechanism of organic selenium on quorum sensing, biofilm, and antioxidant effects of Lactobacillus paracasei

Lou,

Dong,

Tao

et al. 2024

Cell Biochemistry & Function

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Different organic compounds can have varying degrees of impact on the activity of Lactobacillus paracasei. The study focused on the impact and action mechanism of different organic selenium products on the bioactivity of two strains of L. paracasei. The growth, antioxidant activity, extracellular polysaccharide secretion, quorum sensing (QS), and biofilm formation of the strains before and after the addition of organic selenium crude products and three organic selenium standard were evaluated. The results showed that the addition of crude organic selenium promoted the various activities of the strain. l‐selenocysteine had the strongest regulatory effect, with maximum GIM1.80 biofilm formation when it reached a critical concentration of 0.4 μg/mL; l‐selenomethionine resulted in the highest activity of the signal molecule Auto inducer‐2 of GDMCC1.155, when it reached a critical concentration of 0.4 μg/mL. The results of scanning electron microscopy demonstrated that the addition of organic selenium effectively improved the morphological structure of the two bacterial cells. Molecular docking revealed that the mechanism by which organic selenium regulates QS in Lactobacillus was achieved by binding two crucial receptor proteins (histidine protein kinase HKP and periplasmic binding protein LuxP) from specific sites. Furthermore, organic selenium products have a beneficial regulatory effect on the biological activity of L. paracasei. Overall, these findings provide a new alternative (organic selenium) for regulating the viability and beneficial activity of L. paracasei.

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Improve the viability and extracellular polymeric substances bioactivity of Lactiplantibacillus plantarum VAL6 using the environmental adaptation

Cited by 8 publications

References 45 publications

Application of pre-adaptation strategies to improve the growth of probiotic lactobacilli under food-relevant stressful conditions

Application of pre-adaptation strategies to improve the growth of probiotic lactobacilli under food-relevant stressful conditions

Efficacy of the incorporation between self-encapsulation and cryoprotectants on improving the freeze-dried survival of probiotic bacteria

Regulation and mechanism of organic selenium on quorum sensing, biofilm, and antioxidant effects of Lactobacillus paracasei

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