Imprinting of bovine serum albumin in a nonwoven polypropylene membrane supported polyacrylamide/calcium alginate interpenetrating polymer network hydrogel

Kan, Bohong; Lin, Beibei; Zhao, Kongyin; Zhang, Xinxin; Feng, Lingzhi; Wei, Junfu; Ying-chang, Fan

doi:10.1039/c4ra09364j

Cited by 11 publications

(8 citation statements)

References 32 publications

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“…Although hydrogels MIPs have high selectivity to template proteins, proteins diffuse very slowly in hydrogels. Surface imprinting can be used to solve the problem of protein diffusion [26,27,28]. Toru Shiomi et al [29] synthesized hemoglobin (Hb) molecularly imprinted polymer on silica using 3-aminopropyltrimethoxysilane and trimethoxypropylsilane as the functional monomers.…”

Section: Introductionmentioning

confidence: 99%

Adsorption and Electrochemical Detection of Bovine Serum Albumin Imprinted Calcium Alginate Hydrogel Membrane

Zhao

Bao

et al. 2019

Polymers

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In this paper, bovine serum albumin (BSA)-imprinted calcium alginate (CaAlg) hydrogel membrane was prepared using BSA as a template, sodium alginate (NaAlg) as a functional monomer, and CaCl2 as a cross-linker. The thickness of the CaAlg membrane was controlled by a glass rod enlaced with brass wires (the diameter was 0.1, 0.2, 0.3, 0.4, and 0.5 mm). The swelling properties of the CaAlg membranes prepared with different contents of NaAlg were researched. Circular dichroism indicated that the conformation of BSA did not change during the preparing and eluting process. The thinner the CaAlg hydrogel membrane was, the larger the adsorption capacity and the higher the imprinting efficiency of the CaAlg. The maximum adsorption capacity of molecularly imprinted polymer (MIP) and non-imprinted CaAlg hydrogel membrane (NIP) was 38.6 mg·g−1 and 9.2 mg·g−1, respectively, with an imprinting efficiency of 4.2. The MIP was loaded on the electrode to monitor the selective adsorption of BSA by voltammetry curve.

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Section: Introductionmentioning

confidence: 99%

Adsorption and Electrochemical Detection of Bovine Serum Albumin Imprinted Calcium Alginate Hydrogel Membrane

Zhao

Bao

et al. 2019

Polymers

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“…MIPs have been well applied in separation, antibody mimics, biosensors, enzyme catalysis and other areas owing to their chemical stability, organic solvent resistance, high specificity and antibody‐like binding properties or enzyme‐like activities . However, there are also some challenges for bio‐macromolecules, especially proteins, due to the structural complexity, conformational flexibility, large molecular size and poor solubility in water . Accordingly, more promising alternative techniques have been exploited to overcome these barriers from different viewpoints .…”

Section: Introductionmentioning

confidence: 99%

“…[3][4][5][6][7][8][9] However, there are also some challenges for bio-macromolecules, especially proteins, due to the structural complexity, conformational flexibility, large molecular size and poor solubility in water. [10][11][12][13] Accordingly, more promising alternative techniques have been exploited to overcome these barriers from different viewpoints. [14][15][16][17][18][19][20] Surface imprinting is one of the most widely used methods, which locates all affinity recognition sites on the polymer surfaces; thus the diffusion of large macromolecules into and out of the network is facilitated.…”

Section: Introductionmentioning

confidence: 99%

Preparation of pH and temperature dual‐sensitive molecularly imprinted polymers based on chitosan and N‐isopropylacrylamide for recognition of bovine serum albumin

Dong

Hou

et al. 2019

Polymer International

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pH and temperature dual‐sensitive protein imprinted microspheres with high absorption capacity have been successfully synthesized on the surface of SiO2 using chitosan grafted N‐isopropylacrylamide (CS‐g‐NIPAM) as the pH and temperature sensitive monomer, with acrylamide as comonomer, N,N′‐methylenebisacrylamide as the crosslinking agent and bovine serum albumin (BSA) as the template protein. The pH and temperature dual‐sensitivity was also investigated. The results showed that the adsorption capacity and imprinting factor improved slowly with increasing incubation pH from 4.6 to 7.0, and then decreased sharply in alkaline conditions due to the reduction of non‐specific binding from electrostatic and hydrogen bonding interactions. Fourier transform infrared spectroscopy, thermogravimetric analysis and transmission electron microscopy were used to characterize the polymers. The as‐prepared SiO2@BSA molecularly imprinted polymers were also found to have high adsorption capacity (119.88 mg g−1) within 2 h, an excellent imprinting factor (α = 2.25), specific selectivity and good reusability. © 2019 Society of Chemical Industry

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“…In addition, the diffusion of protein in the hydrogel is quite slow. Surface imprinting technique can be applied to solve the problem of protein diffusion [ 24 , 25 , 26 ]. As functional monomers, silanes and its derivatives were employed to synthetize molecular-imprinted polysiloxane on the surface of solid substrate.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Protein Molecular-Imprinted Polysiloxane Membrane Using Calcium Alginate Film as Matrix and Its Application for Cell Culture

Liu

Zhao

et al. 2018

Polymers

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Bovine serum albumin (BSA) molecular-imprinted polysiloxane (MIP) membrane was prepared by sol-gel technology, using silanes as the functional monomers, BSA as the template and CaAlg hydrogel film as the matrix. The stress-strain curves of wet CaAlg membrane and molecular-imprinted polysiloxane membrane were investigated. We evaluate the adsorption and recognition properties of MIP membrane. Results showed that the adsorption capacity of BSA-imprinted polysiloxane for BSA reached 28.83 mg/g, which was 2.18 times the non-imprinted polysiloxane (NIP) membrane. The adsorption rate was higher than that of the protein-imprinted hydrogel. BSA-imprinted polysiloxane membrane could identify the protein template from competitive proteins such as bovine hemoglobin, ovalbumin and bovine γ-globulin. In order to obtain the biomaterial that can promote cell adhesion and proliferation, fibronectin (FN)-imprinted polysiloxane (FN-MIP) membrane was obtained by using fibronectin as the template, silanes as functional monomers, and CaAlg hydrogel membrane as the substrate or matrix. The FN-MIP adsorbed more FN than NIP. The FN-imprinted polysiloxane membrane was applied to culture mouse fibroblast cells (L929) and the results proved that the FN-MIP had a better effect on cell adhesion than NIP.

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Imprinting of bovine serum albumin in a nonwoven polypropylene membrane supported polyacrylamide/calcium alginate interpenetrating polymer network hydrogel

Abstract: A non-woven polypropylene supported bovine serum albumin imprinted calcium alginate/polyacrylamide hydrogel film with good regeneration performance.

Cited by 11 publications

References 32 publications

Adsorption and Electrochemical Detection of Bovine Serum Albumin Imprinted Calcium Alginate Hydrogel Membrane

Adsorption and Electrochemical Detection of Bovine Serum Albumin Imprinted Calcium Alginate Hydrogel Membrane

Preparation of pH and temperature dual‐sensitive molecularly imprinted polymers based on chitosan and N‐isopropylacrylamide for recognition of bovine serum albumin

Preparation of Protein Molecular-Imprinted Polysiloxane Membrane Using Calcium Alginate Film as Matrix and Its Application for Cell Culture

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