Imprinting control region (ICR) of the Peg3 domain

Kim, Joomyeong; Ekram, Muhammad B.; Kim, Hana; Faisal, Mohammad; Frey, Wesley D.; Huang, Jennifer M.; Tran, Kim Ngoc; Kim, Michelle M.; Yu, Sungryul

doi:10.1093/hmg/dds092

Cited by 43 publications

(77 citation statements)

References 32 publications

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“…53,54 Thus, ICR mice were chosen to be the ideal model for this experiment. In this study, C6 GSCs were implanted into mice brain tissues to construct animal models because C6 GSCs showed better proliferative and invasive abilities compared to C6 glioma cells.…”

Section: Discussionmentioning

confidence: 99%

The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals

Wang

Xue

et al. 2017

IJN

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Glioblastoma is a kind of malignant gliomas that is almost impossible to cure due to the poor drug transportation across the blood–brain barrier and the existence of glioma stem cells. We prepared a new kind of targeted liposomes in order to improve the drug delivery system onto the glioma cells and induce the apoptosis of glioma stem cells afterward. In this experiment, curcumin was chosen to kill gliomas, while quinacrine was used to induce apoptosis of the glioma stem cells. Also, p -aminophenyl-α-D-mannopyranoside could facilitate the transport of liposomes across the blood–brain barrier and finally target the brain glioma cells. The cell experiments in vitro indicated that the targeted liposomes could significantly improve the anti-tumor effects of the drugs, while enhancing the uptake effects, apoptosis effects, and endocytic effects of C6 glioma cells and C6 glioma stem cells. Given the animal experiments in vivo, we discovered that the targeted liposomes could obviously increase the survival period of brain glioma-bearing mice and inhibit the growth of gliomas. In summary, curcumin and quinacrine liposomes modified with p -aminophenyl-α-D-mannopyranoside is a potential preparation to treat brain glioma cells and brain glioma stem cells.

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Section: Discussionmentioning

confidence: 99%

The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals

Wang

Xue

et al. 2017

IJN

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“…These features are often associated with other ICRs, such as the ICR of H19 / Igf2 [11]. A series of subsequent analyses indeed confirmed ICR roles for the Peg3-DMR and also the involvement of Yy1 in the transcription control and DNA methylation of the Peg3 domain [15–18]. In particular, the reduced levels of YY1 protein have been shown to up-regulate the expression levels of the Peg3 domain and also to change the DNA methylation levels of the Peg3-DMR [15–17].…”

Section: Introductionmentioning

confidence: 88%

Yy1 Gene Dosage Effect and Bi-Allelic Expression of Peg3

2015

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In the current study, we tested the in vivo effects of Yy1 gene dosage on the Peg3 imprinted domain with various breeding schemes utilizing two sets of mutant alleles. The results indicated that a half dosage of Yy1 coincides with the up-regulation of Peg3 and Zim1, suggesting a repressor role of Yy1 in this imprinted domain. This repressor role of Yy1 is consistent with the observations derived from previous in vitro studies. The current study also provided an unexpected observation that the maternal allele of Peg3 is also normally expressed, and thus the expression of Peg3 is bi-allelic in the specific areas of the brain, including the choroid plexus, the PVN (Paraventricular Nucleus) and the SON (Supraoptic Nucleus) of the hypothalamus. The exact roles of the maternal allele of Peg3 in these cell types are currently unknown, but this new finding confirms the previous prediction that the maternal allele may be functional in specific cell types based on the lethality associated with the homozygotes for several mutant alleles of the Peg3 locus. Overall, these results confirm the repressor role of Yy1 in the Peg3 domain and also provide a new insight regarding the bi-allelic expression of Peg3 in mouse brain.

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“…The cluster is located on mouse proximal chromosome 7 (101,111). Imprinting in the region is controlled by the Peg3 DMR; this region is located at its promoter, which is hypermethylated during oogenesis but remains hypomethylated during spermatogenesis (106). Study of the Peg3 domain using UPDs is complicated by the presence of other imprinted regions on mouse chromosome 7.…”

Section: Peg3 Domain: Mouse Chromosome 7 (Human Chromosome 19)mentioning

confidence: 99%

“…Study of the Peg3 domain using UPDs is complicated by the presence of other imprinted regions on mouse chromosome 7. However, there is a mouse model that deletes the Peg3 ICR: Maternal transmission of this deletion results in animals that are transiently heavier than controls at weaning (106), whereas paternal transmission results in partial embryonic lethality and reduced body weight (106).…”

Section: Peg3 Domain: Mouse Chromosome 7 (Human Chromosome 19)mentioning

confidence: 99%

Phenotypic Outcomes of Imprinted Gene Models in Mice: Elucidation of Pre- and Postnatal Functions of Imprinted Genes

Cleaton¹,

Edwards

Ferguson-Smith

2014

Annu. Rev. Genom. Hum. Genet.

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Genomic imprinting is an epigenetic process causing expression of a subset of genes in a parent-of-origin-specific manner. Among vertebrates, only therian mammals have been demonstrated to imprint, indicating that placentation and imprinting arose at similar time points in evolution and that imprinting may be involved in key mammal-specific processes. However, although several theories have been posited to explain the evolution of imprinting, each has shortcomings and none fully explains the wide variety of genes regulated by imprinting. In this review, we catalog the phenotypes associated with genetic mutation and overexpression at particular imprinted loci in order to consider the wide impact of imprinted genes on development. In addition to the well-described roles of imprinted genes in prenatal growth and placentation, more recent data emphasize that imprinted genes are critical for specific aspects of postnatal mammalian development involving adaptive processes, metabolism, and behavior.

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Imprinting control region (ICR) of the Peg3 domain

Cited by 43 publications

References 32 publications

The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals

The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals

Yy1 Gene Dosage Effect and Bi-Allelic Expression of Peg3

Phenotypic Outcomes of Imprinted Gene Models in Mice: Elucidation of Pre- and Postnatal Functions of Imprinted Genes

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