Abstract:Aim: Functional roles of erythrocytes on platelet adhesion to vessel wall under blood flow condition is still to be elucidated. Methods: Blood specimens containing native, biochemically fixed, or artificial erythrocytes, at various hematocrits were perfused on immobilized von Willebrand factor (VWF) at a shear rate of 1,500 s− 1. Number of platelets adhered on VWF within the region of interest (ROI: 5x103 µm2) was serially measured for 2 minutes using the fluorescent microscopy system. Regression analyses were… Show more
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