Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity

Lindinger, Peter W.; Christe, Martine; Eberlé, Alex N.; Kern, Beatrice; Peterli, Ralph; Peters, Thomas; Jayawardene, Kamburapola Ji; Fearnley, Ian M.; Walker, John E.

doi:10.1016/j.jprot.2015.03.037

Cited by 35 publications

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References 37 publications

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“…Supplementary Table 1 contains a list of all 126 proteins found and includes also proteins of non-mitochondrial origin. Supplementary Table 2 contains all 62 mitochondrial proteins with potentially different abundance [1] .…”

Section: Data Experimental Design Materials and Methodsmentioning

confidence: 99%

Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity

et al. 2015

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Obesity is associated with impaired mitochondrial function. This study compares mitochondrial protein expression in omental fat in obese and non-obese humans. Omental adipose tissue was obtained by surgical biopsy, adipocytes were purified and mitochondria isolated. Using anion-exchange chromatography, SDS-PAGE and mass-spectrometry, 128 proteins with potentially different abundances in patient groups were identified, 62 of the 128 proteins are mainly localized in the mitochondria. Further quantification of 12 of these 62 proteins by immune dot blot analysis revealed four proteins citrate synthase, HADHA, LETM1 and mitofilin being inversely associated with BMI, and mitofilin being inversely correlated with gender.

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Section: Data Experimental Design Materials and Methodsmentioning

confidence: 99%

Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity

et al. 2015

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“…Proteomic studies revealed that BMI was inversely associated with four important omental adipose tissue mitochondrial proteins—citrate synthase, HADHA, LETM1, and mitofilin . A lower abundance of mitochondrial proteins in subcutaneous adipose tissue has been recorded in insulin resistance without the presence of obesity and in visceral fat of individuals with type 2 diabetes .…”

Section: Mitochondrial Metabolism In White At In Health and In Obesitymentioning

confidence: 99%

White adipose tissue mitochondrial metabolism in health and in obesity

et al. 2019

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White adipose tissue is one of the largest organs of the body. It plays a key role in whole-body energy status and metabolism; it not only stores excess energy but also secretes various hormones and metabolites to regulate body energy balance. Healthy adipose tissue capable of expanding is needed for metabolic well-being and to prevent accumulation of triglycerides to other organs. Mitochondria govern several important functions in the adipose tissue. We review the derangements of mitochondrial function in white adipose tissue in the obese state. Downregulation of mitochondrial function or biogenesis in the white adipose tissue is a central driver for obesity-associated metabolic diseases. Mitochondrial functions compromised in obesity include oxidative functions and renewal and enlargement of the adipose tissue through recruitment and differentiation of adipocyte progenitor cells. These changes adversely affect whole-body metabolic health. Dysfunction of the white adipose tissue mitochondria in obesity has long-term consequences for the metabolism of adipose tissue and the whole body. Understanding the pathways behind mitochondrial dysfunction may help reveal targets for pharmacological or nutritional interventions that enhance mitochondrial biogenesis or function in adipose tissue. KEYWORDS adipose tissue, mitochondria, obesity 1 | INTRODUCTION Obesity is a global and rapidly increasing problem, tripled since 1975 by WHO 2018 standards in developed countries. Obesity is also extremely difficult to treat. A key defining feature of obesity is an adipose tissue dysfunction, which is considered to be a major contributor to the development of obesity-related metabolic problems, 1,2 such as metabolic syndrome, insulin resistance, hypertension, dyslipidaemia, and fatty liver. The underlying pathological mechanisms that impair adipose tissue function in obesity are incompletely understood, but in the light of recent scientific advances, it may be connected to insufficient storage capacity or impaired function of mitochondria, or both. Mitochondria are the energy centres of adipocytes and are involved in many of their key metabolic functions including ATP production, fatty acid synthesis and oxidation, and the triglyceride balance of the cell. Although adipose tissue was long considered as an inert reservoir of fat with low abundance of mitochondria, adipose tissue and its active mitochondria have recently emerged as one of the central regulators influencing whole-body metabolism. 1,3,4 Impairments in adipocyte mitochondrial function are associated with metabolic diseases and the development of obesity-related disorders. 3-6Better understanding on the dysfunction of adipose tissue mitochondria may yield insights on how the metabolic complications of obesity could be reversed. In this review, we concentrate on the metabolic processes in white adipose tissue that are regulated by mitochondria /journal/obr 1 of 23 23 of like bariatric surgery that seem to preserve a favourable metabolic profile in adipose tissue should be ...

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“…In 2015, Lindinger et al published the first mitoproteomics study on this area [ 152 ]. Despite the limitations of their analysis (e.g., only 62 putative mitochondrial proteins were identified), the authors validated four proteins (citrate synthase, mitofilin, HADHA, and LETM1) that were inversely associated with body mass index (BMI) in white adipocyte mitochondria from 76 patients.…”

Section: Addressing Mitochondrial Dysfunction Through High-throughmentioning

confidence: 99%

Mitoproteomics: Tackling Mitochondrial Dysfunction in Human Disease

Gómez-Serrano

Camafeita

Loureiro

et al. 2018

Oxidative Medicine and Cellular Longevity

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Mitochondria are highly dynamic and regulated organelles that historically have been defined based on their crucial role in cell metabolism. However, they are implicated in a variety of other important functions, making mitochondrial dysfunction an important axis in several pathological contexts. Despite that conventional biochemical and molecular biology approaches have provided significant insight into mitochondrial functionality, innovative techniques that provide a global view of the mitochondrion are still necessary. Proteomics fulfils this need by enabling accurate, systems-wide quantitative analysis of protein abundance. More importantly, redox proteomics approaches offer unique opportunities to tackle oxidative stress, a phenomenon that is intimately linked to aging, cardiovascular disease, and cancer. In addition, cutting-edge proteomics approaches reveal how proteins exert their functions in complex interaction networks where even subtle alterations stemming from early pathological states can be monitored. Here, we describe the proteomics approaches that will help to deepen the role of mitochondria in health and disease by assessing not only changes to mitochondrial protein composition but also alterations to their redox state and how protein interaction networks regulate mitochondrial function and dynamics. This review is aimed at showing the reader how the application of proteomics approaches during the last 20 years has revealed crucial mitochondrial roles in the context of aging, neurodegenerative disorders, metabolic disease, and cancer.

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Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity

Cited by 35 publications

References 37 publications

Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity

Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity

White adipose tissue mitochondrial metabolism in health and in obesity

Mitoproteomics: Tackling Mitochondrial Dysfunction in Human Disease

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