Importance of Utilizing Natural Isotopologue Transitions in Expanding the Linear Dynamic Range of LC-MS/MS Assay for Small-Molecule Pharmacokinetic Sample Analysis – A Mini-review

Bach, Thanh; An, Guohua

doi:10.1016/j.xphs.2021.12.012

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…In the quantitative analysis of biological samples, the deuterated standard was the best internal standard to correct for loss in the extraction process [25]. However, this internal standard was expensive and not always available for purchase.…”

Section: Methodology Developmentmentioning

confidence: 99%

Pharmacokinetics of Ziyuglycoside I and Ziyuglycoside II in Rat Plasma by UPLC-MS/MS

Shen,

Wang,

et al. 2024

International Journal of Analytical Chemistry

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Ziyuglycoside I and ziyuglycoside II are important active components of Sanguisorba officinalis L., which have excellent pharmacological effects, such as antioxidant and anticancer effects. However, the bioavailability of ziyuglycoside I and ziyuglycoside II has not been reported. This work aims to establish a UPLC-MS/MS method to study the pharmacokinetics of ziyuglycoside I and ziyuglycoside II in rats under different administration routes (intragastric and intravenous administration) and to calculate the bioavailability. The concentration of ziyuglycoside I and ziyuglycoside II in rat plasma in the range of 2–2000 ng/mL showed a good linear relationship (r > 0.99). The intra-day accuracies of ziyuglycoside I and ziyuglycoside II ranged from 87% to 110%, and the inter-day accuracies ranged from 97% to 109%. The intra-day precision was less than 15% and the inter-day precision was less than 14%. The matrix effects ranged from 88% to 113%. The recoveries were all above 84%. The developed UPLC-MS/MS method for the determination of ziyuglycoside I and ziyuglycoside II in rat plasma was applied to pharmacokinetics. The bioavailability of ziyuglycoside I and ziyuglycoside II was measured at 2.6% and 4.6%, respectively.

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Section: Methodology Developmentmentioning

confidence: 99%

Pharmacokinetics of Ziyuglycoside I and Ziyuglycoside II in Rat Plasma by UPLC-MS/MS

Shen,

Wang,

et al. 2024

International Journal of Analytical Chemistry

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“…LC-MS/MS is an analytical chemistry technique that includes physical separation of the analytes in liquid samples and followed by their mass-based identification ( 27 ). This technology contributed to the determination of the accurate mass, putative formula, and elucidate the structure of the analyte with relatively small amounts of samples.…”

Section: Application Of Proteomics To Identify Predictive Biomarkers ...mentioning

confidence: 99%

Application of Proteomics in the Discovery of Radiosensitive Cancer Biomarkers

Luo

2022

Front. Oncol.

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Radiation therapy remains an important component of cancer treatment. Gene-encoded proteins were the actual executors of cellular functions. Proteomic was a novel technology that can systematically analysis protein composition and measure their levels of change, this was a high throughput method, and were the import tools in the post genomic era. In recent years, rapid progress of proteomic have been made in the study of cancer mechanism, diagnosis, and treatment. This article elaborates current advances and future directions of proteomics in the discovery of radiosensitive cancer biomarkers.

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“…High-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) technology has the advantages of high sensitivity, low detection limit, and small sample consumption and is widely used in drug analysis of chemical composition, drug metabolism, and impurity identification [ 17 , 18 ]. UPLC, which columns with small particle sizes and under ultra-high pressure, maintains the basic principles of a traditional HPLC system but demonstrates improved separation efficiency and speed [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Determination of Tenacissoside G, Tenacissoside H, and Tenacissoside I in Rat Plasma by UPLC-MS/MS and Their Pharmacokinetics

Chen,

Ma,

Cui

et al. 2023

International Journal of Analytical Chemistry

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An ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of tenacissoside G, tenacissoside H, and tenacissoside I in rat plasma. The rat plasma was treated with liquid-liquid extraction using ethyl acetate. The determination was performed on the UPLC HSS T3 column (50 mm × 2.1 mm, 1.8 μm) with a mobile phase consisting of acetonitrile-water (containing 0.1% formic acid) and gradient elution at a flow rate of 0.4 mL/min. Electrospray (ESI) positive ion mode detection and multireaction monitoring (MRM) quantitative analysis were performed. A total of 36 rats were given tenacissoside G, tenacissoside H, and tenacissoside I, respectively, orally (5 mg/kg) and intravenously (1 mg/kg), with 6 rats in each group, to evaluate the pharmacokinetic difference of tenacissoside G, tenacissoside H, and tenacissoside I in rats. The calibration curves showed good linearity in the range of 5–2000 ng/mL, where r was greater than 0.99. The results of precision, accuracy, recovery, matrix effect, and stability met the requirements of biological sample detection methods. The established UPLC-MS/MS method was successfully applied to pharmacokinetic studies of tenacissoside G, tenacissoside H, and tenacissoside I, and the bioavailability was 22.9%, 89.8%, and 9.4%, respectively.

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Importance of Utilizing Natural Isotopologue Transitions in Expanding the Linear Dynamic Range of LC-MS/MS Assay for Small-Molecule Pharmacokinetic Sample Analysis – A Mini-review

Cited by 3 publications

References 16 publications

Pharmacokinetics of Ziyuglycoside I and Ziyuglycoside II in Rat Plasma by UPLC-MS/MS

Pharmacokinetics of Ziyuglycoside I and Ziyuglycoside II in Rat Plasma by UPLC-MS/MS

Application of Proteomics in the Discovery of Radiosensitive Cancer Biomarkers

Determination of Tenacissoside G, Tenacissoside H, and Tenacissoside I in Rat Plasma by UPLC-MS/MS and Their Pharmacokinetics

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