Importance of the galE gene on the virulence of Pasteurella multocida

Henestrosa, Antonio R. Fernández de; Badiola, Ignacio; Saco, Montserrat; Rozas, Ana Pérez de; Campoy, Susana; Barbé, Jordi

doi:10.1111/j.1574-6968.1997.tb12661.x

Cited by 12 publications

(10 citation statements)

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“…However, it is possible that disruption of the FTT0791 gene introduces a pleiotropic phenotype that could indirectly affect the biosynthesis of other polysaccharides. We sought to address (27,60) and reduced virulence of a galE mutant of Pasteurella multocida in mice (15). However, in our study, the FTT0791 and FTT0798 mutants were not attenuated in a murine model of tularemia.…”

Section: Discussionmentioning

confidence: 83%

Glycosylation of DsbA in Francisella tularensis subsp. tularensis

et al. 2011

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In Francisella tularensis subsp. tularensis, DsbA has been shown to be an essential virulence factor and has been observed to migrate to multiple protein spots on two-dimensional electrophoresis gels. In this work, we show that the protein is modified with a 1,156-Da glycan moiety in O-linkage. The results of mass spectrometry studies suggest that the glycan is a hexasaccharide, comprised of N-acetylhexosamines, hexoses, and an unknown monosaccharide. Disruption of two genes within the FTT0789-FTT0800 putative polysaccharide locus, including a galE homologue (FTT0791) and a putative glycosyltransferase (FTT0798), resulted in loss of glycan modification of DsbA. The F. tularensis subsp. tularensis ⌬FTT0798 and ⌬FTT0791::Cm mutants remained virulent in the murine model of subcutaneous tularemia. This indicates that glycosylation of DsbA does not play a major role in virulence under these conditions. This is the first report of the detailed characterization of the DsbA glycan and putative role of the FTT0789-FTT0800 gene cluster in glycan biosynthesis.

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Section: Discussionmentioning

confidence: 83%

Glycosylation of DsbA in Francisella tularensis subsp. tularensis

et al. 2011

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“…TEMPERATE TRANSDUCING PHAGE FOR PASTEURELLA MULTOCIDA 3155 (strain PM1090) were determined in triplicate as reported previously (12). Basically, groups of five mice were injected intraperitoneally with 0.1 ml of serial 10-fold dilutions of bacteria in buffered peptone water.…”

Section: Vol 72 2006mentioning

confidence: 99%

Isolation and Sequencing of a Temperate Transducing Phage forPasteurella multocida

Campoy

Aranda

Álvarez

et al. 2006

Appl Environ Microbiol

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A temperate bacteriophage (F108) has been isolated through mitomycin C induction of a Pasteurella multocida serogroup A strain. F108 has a typical morphology of the family Myoviridae, presenting a hexagonal head and a long contractile tail. F108 is able to infect all P. multocida serogroup A strains tested but not those belonging to other serotypes. Bacteriophage F108, the first P. multocida phage sequenced so far, presents a 30,505-bp double-stranded DNA genome with cohesive ends (CTTCCTCCCC cos site). The F108 genome shows the highest homology with those of Haemophilus influenzae HP1 and HP2 phages. Furthermore, an F108 prophage attachment site in the P. multocida chromosome has been established to be inside a gene encoding tRNA Leu . By using several chromosomal markers that are spread along the P. multocida chromosome, it has been demonstrated that F108 is able to perform generalized transduction. This fact, together with the absence of pathogenic genes in the F108 genome, makes this bacteriophage a valuable tool for P. multocida genetic manipulation.Pasteurella multocida is a gram-negative bacterium belonging to the class of gammaproteobacteria that is responsible for causing diseases in many species of mammals and birds, resulting in important health problems in the animal production industry (5). Diseases induced by P. multocida include hemorrhagic septicemia in cattle, atrophic rhinitis in swine, and fowl cholera in wild and domestic birds (5). Five different P. multocida serogroups (A, B, D, E, and F) have been described based on the antigenicity of the capsule (3, 5). It is known that serogroup A strains are mainly involved in fowl cholera, whereas serogroups B and E are implicated in hemorrhagic septicemia and serogroup D seems to be responsible for atrophic rhinitis (7,22,33,37). Because of the economic relevance of P. multocida-mediated infections, work in several fields concerning this bacterial species, such as molecular characterization of pathogenic factors and construction of mutants to be used as vaccines, is increasing. Nevertheless, few genetic tools for P. multocida genetic manipulation are available (1,4,32).Temperate bacteriophages are excellent tools for genetic manipulation of bacteria. They may be useful for carrying out generalized transduction and for development of cloning vectors (9, 13, 16, 35). Several P. multocida bacteriophages were described in the past, but they were used only for typing proposals (19). To our knowledge, no transducing bacteriophages have been described for P. multocida so far. Moreover, no P. multocida phage has been completely sequenced.In this context, we have characterized a temperate bacteriophage (F108) obtained from a P. multocida serogroup A isolate that is able to carry out generalized transduction. This bacteriophage has also been sequenced, and the absence of pathogenic factors in its genome has been demonstrated by both in silico and in vivo experimental methods. These data suggest that bacteriophage F108 may be a suitable genetic tool for P. mul...

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“…They are composed of repeating units of monosaccharides linked by glycosidic bonds (Brock et al 1997). In gram negative bacteria, the lipopolysaccharide (LPS) layer is a major surface component, present only in the outer leaflet of the outer membrane and serves as one of the main virulence factors of these bacteria (Roberts 1996;Fernández de Henestrosa et al 1997;Boyce et al 2000). The LPS molecule consists of three distinct regions.…”

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confidence: 99%

“…An important constituent of the LPS core is galactose (Fernández de Henestrosa et al 1997). According to the Leloir pathway, galactose metabolism requires the epimerization of uridine diphosphate (UDP)-glucose to a galactose derivative UDP-galactose.…”

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confidence: 99%

The cloning and sequencing of the UDP-galactose 4-epimerase gene (galE) fromAvibacterium paragallinarum

2007

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The putative uridine diphosphate (UDP)-galactose 4-epimerase encoding gene, galE, was isolated from Avibacterium paragallinarum with the use of degenerate primers, colony hybridization and inverse PCR. The data revealed an open reading frame of 1017 bp encoding a protein of 338 amino acids with a molecular weight of 37 kDa and an isoelectric point of 5.5. High sequence homology was obtained with an 87, 91 and 89% sequence identity on protein level towards the galE genes from Actinobacillus pleuropneumoniae, Haemophilus influenza and Pasteurella multocida, respectively. To verify that the cloned galE gene encodes for a UDP-galactose 4-epimeras, this gene was cloned into the pYES-2 expression vector, followed by transformation in a Saccharomyces cerevisiae gal10 deletion strain. Complementation of the gal10 deletion mutant with the galE gene confirmed that this gene encodes a UDP-galactose 4-epimerase.

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Importance of the galE gene on the virulence of Pasteurella multocida

Cited by 12 publications

References 10 publications

Glycosylation of DsbA in Francisella tularensis subsp. tularensis

Glycosylation of DsbA in Francisella tularensis subsp. tularensis

Isolation and Sequencing of a Temperate Transducing Phage forPasteurella multocida

The cloning and sequencing of the UDP-galactose 4-epimerase gene (galE) fromAvibacterium paragallinarum

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