Importance of the 11β-hydroxysteroid dehydrogenase enzyme in clinical disorders

Feldman, Karolina; Láng, István; Nagy, Zsolt; Szappanos, Ágnes; Grolmusz, Vince; Tóth, Miklós; Rácz, Kàroly; Patócs, Attila

doi:10.1556/oh.2013.29558

Cited by 5 publications

(4 citation statements)

References 48 publications

(102 reference statements)

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“…These findings indicated that DEGs may have a significant impact on regulating TME and influencing the prognosis of ccRCC. Since The HSD11B1 gene encodes the type 1 isoform of 11-β-hydroxysteroid dehydrogenase, which converts glucocorticoids into the active form, which plays an important role in the regulation of metabolic syndrome and immune response [ 21 ]. Further analyses were carried out on HSD11B1 expression profile.…”

Section: Discussionmentioning

confidence: 99%

“…Eventually, a predictive biomarker, hydroxysteroid 11-beta dehydrogenase-1 (HSD11B1) was selected. The HSD11B1 gene encodes the type 1 isoform of 11-β-hydroxysteroid dehydrogenase, which converts glucocorticoids into the active form, which plays an important role in the regulation of cell proliferation, differentiation and immune response [ 21 , 22 ]. Proliferative phenotypes induced by high expression of HSD11B1 are associated with poor cancer prognosis [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Microenvironment-associated gene HSD11B1 may serve as a prognostic biomarker in clear cell renal cell carcinoma: a study based on TCGA, RT‑qPCR, Western blotting, and immunohistochemistry

Han

Hong

et al. 2021

Bioengineered

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Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant tumors worldwide. The clinical treatment of ccRCC is strongly associated with the tumor microenvironment (TME). Identifying potential markers of ccRCC is important to improve prognosis. Therefore, in the present study, the levels of immune/stromal components and the proportion of tumor-infiltrating immune cells (TIICs) were determined in 611 ccRCC samples using the ESTIMATE and CIBERSORT analytical tools. Subsequently, hydroxysteroid 11-beta dehydrogenase-1 (HSD11B1) was identified by univariate Cox regression analysis, protein-protein interaction (PPI) networks and clinical survival analysis to be associated with ccRCC prognosis. At the same time, the abundance of HSD11B1 increased significantly in ccRCC was verified by western blotting, RT‑qPCR and immunostaining analysis. Furthermore, Gene Set Enrichment Analysis (GSEA) and TME suggested that HSD11B1 was involved in TME immune-related status. Taken together, the results of the present study demonstrated that HSD11B1 is a potential prognostic biomarker associated with immune cell infiltration in ccRCC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microenvironment-associated gene HSD11B1 may serve as a prognostic biomarker in clear cell renal cell carcinoma: a study based on TCGA, RT‑qPCR, Western blotting, and immunohistochemistry

Han

Hong

et al. 2021

Bioengineered

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“…It is apparent in rodent and human studies, where there is a greater understanding of the physiological roles of GCs, that chronic overexposure to GCs (whether during development or in the adult) can result in multiple and wide‐ranging adverse effects (as reviewed by Almawi et al 2002; Girod & Brotman, 2004; Walker, 2007; McDonough et al 2008; Feldman et al 2013). These studies highlight the importance of homeostatic regulation through the processes of biosynthesis and catabolism.…”

Section: Discussionmentioning

confidence: 99%

Physiological roles of glucocorticoids during early embryonic development of the zebrafish (Danio rerio)

Wilson

Matrone

Livingstone

et al. 2013

The Journal of Physiology

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Key points• Glucocorticoids are known to be present in the developing zebrafish embryo but little is known about their physiological role at this early stage.• The zebrafish embryo demonstrates a functional glucocorticoid system from around 48 h post fertilisation.• This system and the stress response is amenable to pharmacological and genetic manipulation in a manner predicted by mammalian physiology.• Glucocorticoids play a key developmental role in hatching, swimming and stress response.• The zebrafish embryo is a relevant model for the study of glucocorticoid physiology.Abstract While glucocorticoids (GCs) are known to be present in the zebrafish embryo, little is known about their physiological roles at this stage. We hypothesised that GCs play key roles in stress response, hatching and swim activity during early development. To test this, whole embryo cortisol (WEC) and corticosteroid-related genes were measured in embryos from 6 to 120 h post fertilisation (hpf) by enzyme linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR). Stress response was assessed by change in WEC following stirring, hypoxia or brief electrical impulses applied to the bathing water. The impact of pharmacological and molecular GC manipulation on the stress response, spontaneous hatching and swim activity at different stages of development was also assessed. WEC levels demonstrated a biphasic pattern during development with a decrease from 0 to 36 hpf followed by a progressive increase towards 120 hpf. This was accompanied by a significant and sustained increase in the expression of genes encoding cyp11b1 (GC biosynthesis), hsd11b2 (GC metabolism) and gr (GC receptor) from 48 to 120 hpf. Metyrapone (Met), an inhibitor of 11β-hydroxylase (encoded by cyp11b1), and cyp11b1 morpholino (Mo) knockdown significantly reduced basal and stress-induced WEC levels at 72 and 120 hpf but not at 24 hpf. Spontaneous hatching and swim activity were significantly affected by manipulation of GC action from approximately 48 hpf onwards. We have identified a number of key roles of GCs in zebrafish embryos contributing to adaptive physiological responses under adverse conditions. The ability to alter GC action in the zebrafish embryo also highlights its potential value for GC research.

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“…A CTON CTOL-á való átalakulást a 11-βHSD1 enzim katalizálja, főként a májban, gonádokban, zsírszövetben, agyban és csontban. [14] A TEST DHTT-ná alakul át az 5-alfa-reduktáz enzim hatására a szervezet perifériás szöveteiben, a prosztatában, herékben, a májban és az ováriumban. […”

Section: Egyéb Szervek Szteroid Hormon Termeléseunclassified

Az extrém fizikális és mentális terhelés, valamint az endogén szteroid bioszintézis összefüggései professzionális sportolóknál

Csöndör

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Az élsportolók az edzések, illetve mérkőzések során folyamatos intenzív mentális és fizikai megterhelésnek vannak kitéve. A stressz hatására a hipotalamusz–hipofízis–mellékvese tengely, valamint a hipotalamusz-hipofízis-gonád tengely aktiválódik, így a mellékvesekéregben, gonádokban, perifériákon fokozódik a szteroid hormontermelés. Kutatásunk során célunk volt élsportolók endogén szteroid-profilját vizsgálni extrém fizikai, valamint extrém mentális terhelés hatására. A terhelések során vérvétel történt a terhelés előtt nyugalmi állapotban, a terhelés maximumán, valamint 30 perccel a terhelést követően. A vizsgálati mintákból folyadékkromatográffal kapcsolt tandem tömegspektrométerrel szteroid-profil meghatározást végeztünk, egyidejűleg 16 endogén szteroid hormon koncentrációját határoztuk meg. Akut extrém fizikai terhelés hatására minden vizsgált szteroid hormon legalább egy terhelési fázisában szignifikáns növekedést mutatott. Szelektív aktiválódást extrém fizikai terhelés hatására sem a mellékvesekéreg zónái, sem a szteroid hormonokat termelő szervek nem mutattak. Az élsportolók endogén szteroid hormon szintjeinek legjelentősebb változását extrém akut fizikai terhelésnél a restitúciós fázisban azonosítottuk. Az alkalmazott módszertan alkalmas volt élsportolók endokrin homeosztázisának monitorozására, a terhelésre adott válasz jellemzésére.

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Importance of the 11β-hydroxysteroid dehydrogenase enzyme in clinical disorders

Cited by 5 publications

References 48 publications

Microenvironment-associated gene HSD11B1 may serve as a prognostic biomarker in clear cell renal cell carcinoma: a study based on TCGA, RT‑qPCR, Western blotting, and immunohistochemistry

Microenvironment-associated gene HSD11B1 may serve as a prognostic biomarker in clear cell renal cell carcinoma: a study based on TCGA, RT‑qPCR, Western blotting, and immunohistochemistry

Physiological roles of glucocorticoids during early embryonic development of the zebrafish (Danio rerio)

Az extrém fizikális és mentális terhelés, valamint az endogén szteroid bioszintézis összefüggései professzionális sportolóknál

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