Importance of Stress Response Mechanisms in Filamentous Fungi for Agriculture and Industry

Aghcheh, Razieh Karimi; Braus, Gerhard H.

doi:10.1007/978-3-030-00683-9_6

Cited by 3 publications

(2 citation statements)

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“…Trichoderma is one of the most isolated and studied ascomycetes due to its agro-industrial importance as a biocontrol organism and producer of secondary metabolites with biological activity [ 23 , 24 , 25 ]. These fungi act as antagonistic parasites against plant pathogens by inducing resistance, antibiosis, mycoparasitism and competition, protecting the plant from diseases [ 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Peptaibol Production and Characterization from Trichoderma asperellum and Their Action as Biofungicide

Alfaro-Vargas¹,

Bastos-Salas

Muñoz-Arrieta³

et al. 2022

JoF

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Peptaibols (Paib), are a class of biologically active peptides isolated from soil, fungi and molds, which have interesting properties as antimicrobial agents. Paib production was optimized in flasks by adding sucrose as a carbon source, 2-aminoisobutyric acid (Aib) as an additive amino acid, and F. oxysporum cell debris as an elicitor. Paib were purified, sequenced and identified by High-performance liquid chromatography (HPLC)coupled to mass spectrometry. Afterward, a Paib extract was obtained from the optimized fermentations. The biological activity of these extracts was evaluated using in vitro and in vivo methods. The extract inhibited the growth of specific plant pathogens, and it showed inhibition rates similar to those from commercially available fungicides. Growth inhibition rates were 92.2, 74.2, 58.4 and 36.2% against Colletotrichum gloeosporioides, Botrytis cinerea, Alternaria alternata and Fusarium oxysporum, respectively. Furthermore, the antifungal activity was tested in tomatoes inoculated with A. alternata, the incidence of the disease in tomatoes treated with the extract was 0%, while the untreated fruit showed a 92.5% incidence of infection Scanning electron microscopy images showed structural differences between the fungi treated with or without Paib. The most visual alterations were sunk and shriveled morphology in spores, while the hyphae appeared to be fractured, rough and dehydrated.

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Section: Introductionmentioning

confidence: 99%

Peptaibol Production and Characterization from Trichoderma asperellum and Their Action as Biofungicide

Alfaro-Vargas¹,

Bastos-Salas

Muñoz-Arrieta³

et al. 2022

JoF

View full text Add to dashboard Cite

show abstract

“…Trichoderma is one of the most isolated and studied ascomycetes due to its agro-industrial importance as a biocontrol organism and producer of secondary metabolites with biological activity [23][24][25]. These fungi act as antagonistic parasites against plant pathogens by inducing resistance, antibiosis, mycoparasitism and competition, protecting the plant from diseases [26][27][28]. Furthermore, Trichoderma species are well known Paib producers.…”

Section: Introductionmentioning

confidence: 99%

Peptaibol Production and Characterization from Trichoderma asperellum and their Action as Biofungicide

Alfaro-Vargas¹,

Bastos-Salas

Muñoz-Arrieta³

et al. 2022

Preprint

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Peptaibols (Paib), are a class of biologically active peptides isolated from soil, fungi and molds, which have interesting properties as antimicrobial agents. Paib production was optimized in flasks by adding sucrose as a carbon source, Aib as an additive amino acid, and F. oxysporum cell debris as an elicitor. Paib were purified, sequenced and identified by HPLC coupled to mass spectrometry. Af-terward, a Paib prototype was prepared with the extracts obtained from the optimized fermenta-tions. The biological activity of these prototypes was evaluated using in vitro and in vivo methods. The prototype inhibited the growth of specific plant pathogens, and it showed inhibition rates similar to those from commercially available fungicides. Growth inhibition rates were 92.2, 74.2, 58.4 and 36.2 % against Colletotrichum gloeosporioides, Botrytis cinerea, Alternaria alternata and Fusarium oxysporum, respectively. Furthermore, the antifungal activity was tested in tomatoes inoculated with A. alternata, the incidence of the disease in tomatoes treated with the prototype was 0%, while the untreated fruit showed a 92.5% of infection. Scanning electron microscopy images showed structural differences between fungi treated with or without Paib. The most visual altera-tions were sunk and shriveled morphology in spores, while the hyphae appeared to be fractured, rough, and dehydrated.

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The high osmolarity glycerol (HOG) pathway in fungi^†

Yaakoub

Sánchez

Ongay‐Larios

et al. 2021

Critical Reviews in Microbiology

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Importance of Stress Response Mechanisms in Filamentous Fungi for Agriculture and Industry

Cited by 3 publications

References 147 publications

Peptaibol Production and Characterization from Trichoderma asperellum and Their Action as Biofungicide

Peptaibol Production and Characterization from Trichoderma asperellum and Their Action as Biofungicide

Peptaibol Production and Characterization from Trichoderma asperellum and their Action as Biofungicide

The high osmolarity glycerol (HOG) pathway in fungi^†

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Importance of Stress Response Mechanisms in Filamentous Fungi for Agriculture and Industry

Cited by 3 publications

References 147 publications

Peptaibol Production and Characterization from Trichoderma asperellum and Their Action as Biofungicide

Peptaibol Production and Characterization from Trichoderma asperellum and Their Action as Biofungicide

Peptaibol Production and Characterization from Trichoderma asperellum and their Action as Biofungicide

The high osmolarity glycerol (HOG) pathway in fungi†

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The high osmolarity glycerol (HOG) pathway in fungi^†