Importance of sludge conditioning in attenuating antibiotic resistance: Removal of antibiotic resistance genes by bioleaching and chemical conditioning with Fe[III]/CaO

Zheng, Guanyu; Lu, Yi; Wang, Dianzhan; Zhou, Lixiang

doi:10.1016/j.watres.2018.12.053

Cited by 79 publications

(15 citation statements)

References 59 publications

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“…The 10 aminoglycosides (aadA1, aadA-01, aadA-02, aadA2-01, aadA2-02, aadA2-03, aadA5-01, strB, aac(6′)-Ib-03, and aac(6′)-II), 3 tetracyclines (tet (34), tetG-01, and tetM-01), 2 chloramphenicol (catB3 and f loR), 1 multidrug (mexF), 1 sulfonamide (sul2), and 1 vancomycin (vanC-03) resistance genes in the collected soil samples were monitored to reflect changes in ARG profiles during the land application of various sludge composts, and the 16S rRNA gene copies and 2 MGEs (plasmid IncQ oriV and integron integrase intI1) were also measured to reflect changes in total bacterial biomass and the transfer ability of ARGs, respectively. The abovementioned ARGs and MGEs were abundant in the tested sewage sludge according to the results of our previous study, 13 and they also kept relatively high abundance in the compost products of sewage sludge. 2,24,25 The LightCycler 480 II Real-Time PCR System (Roche, USA) was applied to measure the target genes using the methods mentioned in previous studies.…”

Section: Acs Esandt Engineeringsupporting

confidence: 62%

“…Prior to aerobic composting, sewage sludge should be conditioned and then mechanically dewatered to obtain dewatered sludge cakes with low content of moisture. 2,13,26,27 Thus, effective conditioning, high-efficient dewatering, and aerobic composting form an important technical routine for sewage sludge treatment in China. The conventional conditioning could not effectively reduce the ARG abundance in sewage sludge and the aerobic composting even enriched the sludge-borne ARGs.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The conventional conditioning could not effectively reduce the ARG abundance in sewage sludge and the aerobic composting even enriched the sludge-borne ARGs. 2,13 Fortunately, our previous study found that bioleaching conditioning treatment, which is a biological conditioning approach driven byAcidithiobacillus ferrooxidansto improve the dewaterability of sewage sludge, 28,29 is much more effective than chemical conditioning using polyacrylamide (PAM) or Fe[III]/CaO in reducing the ARG abundance in compost product of conditioned sludge. This mainly resulted from the fact that ARGs in sewage sludge can be removed during bioleaching conditioning and the horizontal transfer of residual ARGs in conditioned sludge can be further limited during the composting of bioleached sludge.…”

Section: ■ Introductionmentioning

confidence: 99%

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Sludge Conditioning Treatments Impact the Fate of Antibiotic Resistance Genes in Agricultural Soils Amended with Sludge Composts

Wang

et al. 2022

ACS EST Eng.

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Sewage sludge is one of the major environmental reservoirs of antibiotic resistance genes (ARGs), while its recycling releases abundant ARGs into the agricultural soils. Sludge conditioning treatment, as an indispensable step to improve sludge dewatering, can enhance the dewaterability of sewage sludge and meanwhile attenuate ARGs in sludge compost, but it remains unclear how sludge conditioning treatments impact the ARG profiles in different agricultural soils amended with sludge composts. In the present study, the fates of 18 ARGs and 2 mobile genetic elements (MGEs) in two types of agricultural soils (i.e., red soil and yellow–brown soil) were investigated for 170 days, after the land application of sludge composts derived from different conditioning treatments, including bioleaching and chemical conditioning using Fe[III]/CaO or polyacrylamide (PAM). The results showed that the absolute abundance of total ARGs and MGEs was the lowest in both the red soil and the yellow–brown soil amended with bioleached sludge compost (Day 170), which were only 26.4–76.8% of that in the corresponding soils amended with the compost products of raw, PAM-conditioned, or Fe[III]/CaO-conditioned sludge. Besides, in comparison with other conditioning treatments, the bioleaching conditioning treatment more greatly limited the enrichment of typical sludge-borne ARGs (sul2, aadA1, aadA2-01, aadA2-02, and aadA2-03) and their potential hosts. It was found that the much less enrichment of sludge-borne ARGs achieved by bioleaching conditioning most probably resulted from both the low abundance of ARGs in bioleached sludge compost and the limited growth of bacteria carrying ARGs in the amended agricultural soils. Therefore, bioleaching conditioning is superior to the chemical conditioning using Fe[III]/CaO or PAM in mitigating antibiotic resistance in different agricultural soils amended with sludge composts, which was contributed by the preremoval of ARGs in sludge compost and the potentially limited growth of bacteria carrying ARGs after the land application of sludge compost.

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Section: Acs Esandt Engineeringsupporting

confidence: 62%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sludge Conditioning Treatments Impact the Fate of Antibiotic Resistance Genes in Agricultural Soils Amended with Sludge Composts

Wang

et al. 2022

ACS EST Eng.

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“…As for global climate change, pollution at this scale may be difficult or impossible to revert, leading to irreversible and devastating consequences on environmental and human health. However, there is still hope, if improved global sanitation, and advanced techniques for water and sludge treatment (Zheng, et al, 2019) prove adequate to attenuate further emergence and dissemination of antibiotic resistance. Global problems require global solutions and only a concerted and sustained international effort can succeed in reversing the ongoing trajectories and trends.…”

Section: Concluding Wordsmentioning

confidence: 99%

Antibiotic resistance: turning evolutionary principles into clinical reality

Andersson

Balaban

Baquero

et al. 2020

FEMS Microbiology Reviews

177

118

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Antibiotic resistance is one of the major challenges facing modern medicine worldwide. The past few decades have witnessed rapid progress in our understanding of the multiple factors that affect the emergence and spread of antibiotic resistance at the population level and the level of the individual patient. However, the process of translating this progress into health policy and clinical practice has been slow. Here, we attempt to consolidate current knowledge about the evolution and ecology of antibiotic resistance into a roadmap for future research as well as clinical and environmental control of antibiotic resistance. At the population level, we examine emergence, transmission and dissemination of antibiotic resistance, and at the patient level, we examine adaptation involving bacterial physiology and host resilience. Finally, we describe new approaches and technologies for improving diagnosis and treatment and minimizing the spread of resistance.

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“…Conditioning was also shown to have an impact on reducing antibiotic‐resistant genes in sludge. Zheng, Lu, Wang, and Zhou (2019) looked at the effects of four different conditioning methods: chemical conditioning with polyacrylamide (PAM), chemical conditioning with Fe + 3/CaO, bioleaching conditioning, and chemical acidification conditioning and found chemical conditioning with Fe +3 /CaO to be most effective at reducing antibiotic‐resistant genes.…”

Section: Sludge To Biosolids: Treatment Advancesmentioning

confidence: 99%

Residuals, sludge, and biosolids: Advancements in the field

Brisolara

Gentile

Puszykowski

et al. 2020

Water Environment Research

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Advancements in the field of residuals, sludge, and biosolids have been made in 2019. This review outlines the major contributions of researchers that have been published in peer-reviewed journals and conference proceedings throughout 2019 and includes brief summaries from over 125 articles. The review is organized in sections including life cycle and risk assessments; characteristics, quality, and measurement including micropollutants, nanoparticles, pathogens, and metals; sludge treatment technologies including dewatering, digestion, composting, and wetlands; disposal and reuse including adsorbents, land application and agricultural uses, nutrient recovery, and innovative uses; odor and air emissions; and energy issues.

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Importance of sludge conditioning in attenuating antibiotic resistance: Removal of antibiotic resistance genes by bioleaching and chemical conditioning with Fe[III]/CaO

Cited by 79 publications

References 59 publications

Sludge Conditioning Treatments Impact the Fate of Antibiotic Resistance Genes in Agricultural Soils Amended with Sludge Composts

Sludge Conditioning Treatments Impact the Fate of Antibiotic Resistance Genes in Agricultural Soils Amended with Sludge Composts

Antibiotic resistance: turning evolutionary principles into clinical reality

Residuals, sludge, and biosolids: Advancements in the field

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