Importance of prumycin produced by <i>Bacillus amyloliquefaciens</i> SD‐32 in biocontrol against cucumber powdery mildew disease

Tanaka, Koujirou; Fukuda, Mutsumi; Amaki, Yusuke; Sakaguchi, Takatoshi; Inai, Koji; Ishihara, Atsushi; Nakajima, Hiromitsu

doi:10.1002/ps.4630

Cited by 41 publications

(26 citation statements)

References 58 publications

(147 reference statements)

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“…Similarly, B. subtilis and B. pumilus were the most effective bio-agent treatments for reducing AUDPC of squash powdery mildew (225.61 and 226.87, respectively) than the control (643.54), while there was insignificant difference of AUDPC in plants treated with T. harzianum, T. viridi, and B. chitinosporus. Obtained results are in agreement with the findings of many researchers who demonstrated that the bio-agents that were used in this study were effective for controlling powdery mildew disease (Kamel 2003;García-Gutiérrez et al 2013 andTanaka et al 2017). Previously, many studies demonstrated that Bacillus spp.…”

Section: Resultssupporting

confidence: 92%

“…Powdery mildew is the most serious disease that attacks squash plants and causes 30-50% of yield losses (El-Naggar et al 2012). The causal agent of cucurbit powdery mildew disease is Podosphaera xanthii, which is one of the most important limiting factors of cucurbit production all over the world (Tanaka et al 2017). Plant diseases are controlled by agreeable means, like resistant varieties, chemical fungicides, natural production, oils, and biological control (Hafez et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Bacillus spp. are safe and have high possibilities of disease control as they are located everywhere in nature and exhibit high thermal tolerance by forming resistant spores (Tanaka et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Biological control of Podosphaera xanthii the causal agent of squash powdery mildew disease by upregulation of defense-related enzymes

Hafez

El-Nagar

Elzaawely

et al. 2018

Egypt J Biol Pest Control

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Squash (Cucurbita pepo L.), one of the most important vegetable crops for human nutrition all over the world, is infected by many diseases, particularly powdery mildew caused by Podosphaera xanthii (syn. Sphaerotheca fuliginea), which is considered the most serious disease causing yield losses. This research study was conducted to investigate the role of Bacillus subtilis, Bacillus chitinosporus, Bacillus pumilus, Bacillus megaterium, Bacillus polymexa, Trichoderma harzianum, and Trichoderma viridi on squash leaves infected with P. xanthii under laboratory and greenhouse conditions. Results indicated that all treatments significantly inhibited the conidial germination of P. xanthii than the control. A significant decrease in the disease symptoms, severity, and the area under disease progress curve (AUDPC) was registered in squash plants sprayed with the tested bio-agents, particularly B. subtilis, B. pumilus, and T. harzianum. The activity of defense-related enzymes, i.e., catalase (CAT), peroxidase (POX), and polyphenol oxidase (PPO), were significantly upregulated as results of most treatments. Light and scanning electron microscopic (SEM) investigation showed that the morphological shape of P. xanthii was abnormal and the pathogen growth was limited in bio-agent-treated plants compared to control plants that showed dramatic infection. Bio-agent treatments significantly increased growth and yield attributes of squash plants over control. Overall, the results showed possibility of using the tested bio-agents to control squash powdery mildew disease as an alternative to fungicides' use that is harmful for human health and polluting the environment.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Biological control of Podosphaera xanthii the causal agent of squash powdery mildew disease by upregulation of defense-related enzymes

Hafez

El-Nagar

Elzaawely

et al. 2018

Egypt J Biol Pest Control

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“…This extract inhibited pathogen growth on plate in low percentages (Sanchez et al 2019). Obtained results are in agreement with many researchers who found that the bio-agents studied were effective for controlling powdery mildew disease (Kamel 2003;García-Gutiérrez et al 2013;Tanaka et al 2017). Table 2 show that all tested treatments significantly reduced the percentage of the disease incidence and severity than the control.…”

Section: Podosphaera Xanthiisupporting

confidence: 89%

Evaluation of biological control agents for managing squash powdery mildew under greenhouse conditions

Elsisi

2019

Egypt J Biol Pest Control

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Squash (Cucurbita pepo L.), is one of the most important vegetable crops for human nutriment in Egypt and the world. One of the most serious diseases that infect squash and cause yield losses was powdery mildew, caused by Podosphaera xanthisii. The aim of this study was to investigate the role of Bacillus subtilis, Paenibacillus polymyxa (10 9 cell ml/1), Trichoderma harzianum, T. album, T. viride and T. hamatum (10 7 spore ml/l) for controlling disease under greenhouse conditions. Results indicated that all treatments significantly inhibited the conidial germination of P. xanthii than control in vitro and decreased the incidence and disease severity after spraying with the bio-agents on squash plants under greenhouse conditions. The fungicide, Topas-100 (10.0% penconazole "w/v" [(R,S-1-(2-(2,4dichlorophenyl)-Q pentyl)-1H-1,2,4-triazole]), followed by B. subtilis was highly significant for decreasing disease incidence (2.8 and 5.3%, respectively) and disease severity percentage (3.5 and 4.8%, respectively) than the control. The activities of biochemical changes, i.e., peroxidase, polyphenol oxidase, and total phenols, were significantly upregulated as results of most treatments. Also, bio-agent treatments caused significant increase in yield characteristics of squash plants such as fruit number/plant and fruit weight/plant than control. B. subtilis recorded the highest increase (110.9% and 98.7%) in fruit number and fruit weight/plant than control.

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“…Bacillus spp. Are distinguished by high temperature tolerance, rapid growth in liquid cultures, ubiquity in soils, and it is considered a safe biocontrol agent to be applied (TANAKA et al, 2017). The mechanisms of control of the antagonist are diverse, standing out the process of antibiosis, competition, parasitism and predation.…”

Section: Introductionmentioning

confidence: 99%

Use of starch manufacturer residue in the production of inoculating biofertilizer

Bueno

Balestri

Rosiello

et al. 2019

REGET

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In the industrialization of cassava, effluents are generated capable of causing great impacts to the environment. One of these residues is called manipueira and its generation occurs by pressing the grated cassava mass. An alternative to the use of this industrial residue is its use as a culture medium in the multiplication of microorganisms useful in agriculture in the form of biological inoculants. The objective of this work was to evaluate the effectiveness of different concentrations of manipueira used as substrate in the development of the bacterium Bacillus subtilis and the fungus Trichoderma spp as biological control agents and plant growth promoters, aiming at the low cost of the agricultural protection process. The tests were carried out by means of a triple plating technique in culture medium containing manipueira, molasses and distilled water in different concentrations for both biological agents. The efficiency of the method, substract and organisms was measured through the calculation of colony forming units (CFU). The maximum production of CFU of the fungus Trichoderma spp was 1.2x109 CFU.mL -1 in a concentration of 175mL.L -1 of manipueira, while for the bacterium Bacillus subtilis, the maximum production reached 6.49x1011 CFU .mL -1 in culture with concentration of 125mL -1 of the residue. Compared to commercial inoculants with concentration around 1x108 CFU, the tested cultures were compatible with this value and still managed to overcome it, confirming its efficiency. Therefore, this is an excellent possibility to direct the use of environmental liabilities and to encourage the use of the biofertilizer by farmers who can produce it in their properties in an alternative way and with a low cost of production in relation to the commercial value.

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Importance of prumycin produced by Bacillus amyloliquefaciens SD‐32 in biocontrol against cucumber powdery mildew disease

Abstract: This study demonstrates that prumycin is a major factor in SD-32's suppression of cucumber powdery mildew disease. Our findings shed light for the first time on prumycin's role in biocontrol by Bacillus against this disease. © 2017 Society of Chemical Industry.

Cited by 41 publications

References 58 publications

Biological control of Podosphaera xanthii the causal agent of squash powdery mildew disease by upregulation of defense-related enzymes

Biological control of Podosphaera xanthii the causal agent of squash powdery mildew disease by upregulation of defense-related enzymes

Evaluation of biological control agents for managing squash powdery mildew under greenhouse conditions

Use of starch manufacturer residue in the production of inoculating biofertilizer

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