Importance of particulate antigen for the induction of dual bronchial reaction in guinea-pigs

Wieslander, Elisabet; Andersson, Paul; Linden, Margareta; Axelsson, Bertil; Källström, Leif; Brattsand, Ralph; Paulsson, I

doi:10.1007/bf01999639

Cited by 25 publications

(10 citation statements)

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“…Recently we described the effect of budesonide (BUD), a new GCS, on an IgE-mediated bronchial anaphylactic reaction in sensitized guinea pigs [8,9]. The present investigation shows that BUD inhibits the allergen-in duced early and late reaction in a recently developed guinea pig model [ 10],…”

Section: Introductionmentioning

confidence: 84%

“…Recently a new model of antigen-induced late al lergic reaction has been developed [10], Briefly the model is characterized as follows. Instillation of a particulate antigen (OA-Sepharose) to guinea pigs sensitized to produce IgE antibodies results in an im mediate anaphylactic bronchial obstruction which declines within 90 min.…”

Section: Effect Of Gcs On Allergen-induced Ige-mediated Late Bronchimentioning

confidence: 99%

“…The experiments were performed as described by Wieslander et al [10]. Briefly, OA covalently coupled to activated C'H-Sepharose 4 B beads (size ~ 100 urn) was instilled to sensitized guinea pigs.…”

Section: Induction O F Later Allergic Reaction In Guinea Pigsmentioning

confidence: 99%

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Effect of Glucocorticosteroid Treatment on Ovalbumin-Induced IgE-Mediated Immediate and Late Allergic Response in Guinea Pig

Andersson¹,

Brange²,

Kogerer³

et al. 1988

Int Arch Allergy Immunol

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The effect of glucocorticosteroid (GCS) treatment on ovalbumine-induced IgE-mediated immediate and late allergic response was studied in sensitized guinea pigs. The results show that the GCS budesonide (BUD) inhibits the allergen-induced IgE-mediated immediate and late bronchial obstruction. The effect on the early reaction is correlated to the inhibition of leukotrienes and histamine release. The importance of mediator release inhibition for the antianaphylactic effect of GCS is discussed. In examining the effect on the late reaction, it was found that BUD had to be present during the early reaction but did not inhibit the early reaction. Furthermore, the effect on the late reaction was correlated to the inhibition of vascular leakage but not to the infiltration of inflammatory cells as examined in bronchoalveolar lavage. The results indicate that some triggering factors important for the development of the late reaction are released during the early reaction. Inhibition of the release of that factor or the activation of inflammatory cells by that factor might be the mechanism behind the antiinflammatory activities of GCS.

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Section: Introductionmentioning

confidence: 84%

Section: Effect Of Gcs On Allergen-induced Ige-mediated Late Bronchimentioning

confidence: 99%

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Effect of Glucocorticosteroid Treatment on Ovalbumin-Induced IgE-Mediated Immediate and Late Allergic Response in Guinea Pig

Andersson¹,

Brange²,

Kogerer³

et al. 1988

Int Arch Allergy Immunol

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“…Antigen immobilization might be one of the facilitating factors for the induction of LPR. Wieslander et al 15 induced secondary allergic response to OA in guinea pigs by coupling the antigen covalently to Sepharose beads. Similarly, adjuvants such as alumen or Freund's adjuvant also retain antigen within the injection point.…”

Section: Discussionmentioning

confidence: 99%

A novel murine model of late‐phase reaction of immediate hypersensitivity

Facincone

Siqueira

Jancar

et al. 1997

Mediators of Inflammation

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We describe here a novel experimental model of late-phase reaction of immediate hypersensitivity developed in mice. It consists of introducing small fragments of heat-coagulated hen egg white into the subcutaneous tissue of mice. After 14 days, animals challenged with purified ovalbumin into the footpad presented an immediate swelling of the paw peaking at 30 min, followed by two peaks of swelling at 6 and 24 h. Histological examination of the paws showed a massive eosinophil infiltration (more than 800 cells/5 microscopic fields). This intense infiltration persisted for more than 14 days after the challenge. Furthermore, in mice immunized with coagulated egg white the delayed swelling of the paws and eosinophilic infiltration were significantly higher than in mice immunized with the classical protocol of ovalbumin in alumen adjuvant. Transfer of lymph node cells obtained from mice implanted with heat-coagulated hen egg white induced footpad swelling and eosinophil infiltration in response to ovalbumin. High levels of ovalbuminspecific IgG1 but not of IgE were detected in the serum of these animals. The advantages of this model for the experimental study of late-phase reaction per se and its relevance to the study of allergic diseases such as asthma are discussed.

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“…Several guinea-pig models of asthma have been developed focusing on both the early and the late phase responses, and on the airways hyperresponsiveness induced either by antigen or environmental and occupational chemicals. Although anaesthetized guinea-pigs have been used to study these phenomena [5][6][7][8], models closer to human asthma have been developed by prolonged measurement of bronchial function in conscious animals using whole body plethysmography [9,10]. However, important methodological differences exist between individual models, in particular with the routes of allergen sensitization and challenge, i.e.…”

mentioning

confidence: 99%