Importance of lipid metabolism on oocyte maturation and early embryo development: Can we apply what we know to buffalo?

Marin, Diego Fernando Dubeibe; Costa, N. N.; Santana, Priscilla di Paula Bessa; Souza, Eduardo Baia de; Ohashi, Otávio Mitio

doi:10.1016/j.anireprosci.2019.106220

Cited by 16 publications

(13 citation statements)

References 120 publications

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“…One particular characteristic of buffalo embryos making vitrification difficult is their high lipid content [ 68 ]. To address this, we supplemented our IVEP medium with L-carnitine, which has been shown to promote lipid metabolism [ 72 , 73 ], provide antioxidative support and improve post-thaw survivability [ 74 , 75 , 76 , 77 ].…”

Section: Discussionmentioning

confidence: 99%

Factors Affecting the Efficiency of In Vitro Embryo Production in Prepubertal Mediterranean Water Buffalo

Currin

Baldassarre

Macedo

et al. 2022

Animals

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Embryos from prepubertal water buffalo can be produced using laparoscopic ovum pickup (LOPU) and in vitro embryo production (IVEP). However, to date, it is unclear what factors and environmental conditions can affect LOPU-IVEP efficiency in prepubertal animals, especially buffalo. In this study, we explored the impact of season, age and individual variation among female donor animals, as well as the effect of the sire used for in vitro fertilization. Donor animals between 2 and 6 months of age were stimulated using gonadotropins prior to LOPU, which was performed at two-week intervals. Following in vitro maturation and fertilization, the resulting embryos were then cultured to the blastocyst stage until they were either vitrified or transferred into recipient animals. The number of follicles available for aspiration and embryo development rates was stable throughout the year. As animals became older, there was a slight trend for fewer COCs recovered from LOPU and better embryo development. There was a large individual variation in both ovarian response and the developmental competence of oocytes among donors. The bull used for fertilization also had a significant impact on embryo development. Upon embryo transfer, pregnancy rates were not affected by the number of embryos transferred per recipient. The best pregnancy rates were achieved when transferring blastocysts, compared to compact morula or hatched blastocysts. Finally, vitrification had no effect on pregnancy rate compared to fresh embryos.

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Section: Discussionmentioning

confidence: 99%

Factors Affecting the Efficiency of In Vitro Embryo Production in Prepubertal Mediterranean Water Buffalo

Currin

Baldassarre

Macedo

et al. 2022

Animals

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“…Some data have indicated that each developmental stage corresponds to the uptake of different fatty acids [ 24 ]; thus, fatty acids play a regulatory role in embryogenesis, affecting not only embryonic metabolism but also oxidative stress, membrane composition, cell signaling events, and gene expression [ 25 ]. Additionally, lipogenesis, lipolysis, and the transport and oxidation of fatty acids occur in embryonic cells of all mammalian species; these processes are intrinsic components of energy metabolism [ 26 ]. Consequently, during embryogenesis, an embryo’s capacity to use exogenous and/or endogenous lipid reserves is important to its development.…”

Section: Discussionmentioning

confidence: 99%

Direct Effects of Mifepristone on Mice Embryogenesis: An In Vitro Evaluation by Single-Embryo RNA Sequencing Analysis

Chen

Lan

et al. 2023

Biomedicines

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The clinical use of mifepristone for medical abortions has been established in 1987 in France and since 2000 in the United States. Mifepristone has a limited medical period that lasts <9 weeks of gestation, and the incidence of mifepristone treatment failure increases with gestation time. Mifepristone functions as an antagonist for progesterone and glucocorticoid receptors. Studies have confirmed that mifepristone treatments can directly contribute to endometrium disability by interfering with the endometrial receptivity of the embryo, thus causing decidual endometrial degeneration. However, whether mifepristone efficacy directly affects embryo survival and growth is still an open question. Some women choose to continue their pregnancy after mifepristone treatment fails, and some women express regret and seek medically unapproved mifepristone antagonization with high doses of progesterone. These unapproved treatments raise the potential risk of embryonic fatality and developmental anomalies. Accordingly, in the present study, we collected mouse blastocysts ex vivo and treated implanted blastocysts with mifepristone for 24 h. The embryos were further cultured to day 8 in vitro to finish their growth in the early somite stage, and the embryos were then collected for RNA sequencing (control n = 3, mifepristone n = 3). When we performed a gene set enrichment analysis, our data indicated that mifepristone treatment considerably altered the cellular pathways of embryos in terms of viability, proliferation, and development. The data indicated that mifepristone was involved in hallmark gene sets of protein secretion, mTORC1, fatty acid metabolism, IL-2-STAT5 signaling, adipogenesis, peroxisome, glycolysis, E2F targets, and heme metabolism. The data further revealed that mifepristone interfered with normal embryonic development. In sum, our data suggest that continuing a pregnancy after mifepristone treatment fails is inappropriate and infeasible. The results of our study reveal a high risk of fetus fatality and developmental problems when pregnancies are continued after mifepristone treatment fails.

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“…It is well known that lipid dysregulation has a strong correlation with both male and female infertility (Pocate-Cheriet et al, 2020). Complete information regarding the biochemical, metabolic and molecular pathways of lipids in oocyte maturation and early embryonic development is scarce (Dubeibe Marin et al, 2019). To be noted, the foundation for existing ART is based on animal studies.…”

Section: Current Challenges and Future Directionsmentioning

confidence: 99%

Role of Lipid Metabolism and Signaling in Mammalian Oocyte Maturation, Quality, and Acquisition of Competence

Khan

Jiang

Hameed

et al. 2021

Front. Cell Dev. Biol.

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It has been found that the quality of oocytes from obese women has been compromised and subsequent embryos displayed arrested development. The compromised quality may be either due to the poor or rich metabolic conditions such as imbalance or excession of lipids during oocyte development. Generally, lipids are mainly stored in the form of lipid droplets and are an important source of energy metabolism. Similarly, lipids are also essential signaling molecules involved in various biological cascades of oocyte maturation, growth and oocyte competence acquisition. To understand the role of lipids in controlling the oocyte development, we have comprehensively and concisely reviewed the literature and described the role of lipid metabolism in oocyte quality and maturation. Moreover, we have also presented a simplified model of fatty acid metabolism along with its implication on determining the oocyte quality and cryopreservation for fertilization.

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Importance of lipid metabolism on oocyte maturation and early embryo development: Can we apply what we know to buffalo?

Cited by 16 publications

References 120 publications

Factors Affecting the Efficiency of In Vitro Embryo Production in Prepubertal Mediterranean Water Buffalo

Factors Affecting the Efficiency of In Vitro Embryo Production in Prepubertal Mediterranean Water Buffalo

Direct Effects of Mifepristone on Mice Embryogenesis: An In Vitro Evaluation by Single-Embryo RNA Sequencing Analysis

Role of Lipid Metabolism and Signaling in Mammalian Oocyte Maturation, Quality, and Acquisition of Competence

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