IMPORTANCE OF LIGHT IN TEMPORAL ORGANIZATION OF PHOTORECEPTOR PROTEINS AND MELATONIN-PRODUCING SYSTEM IN THE PINEAL OF CARP<i>CATLA CATLA</i>

Seth, Mohua; Maitra, Saumen Kumar

doi:10.3109/07420521003666416

Cited by 19 publications

(17 citation statements)

References 60 publications

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“…One category containing the timekeeping AANAT gene was mainly enriched in the two-function model (Figure S7). One of the functions was related to “cytokine response,” including “response to hormone stimulus” and “response to inorganic substance,” and the other function was related to “neuron function,” including “neuron development” and “axonogenesis.” Both of these functions are associated with the circadian clock, and the findings are consistent with previous studies [37–39]. …”

Section: Application and Resultssupporting

confidence: 93%

Stratification of Gene Coexpression Patterns and GO Function Mining for a RNA-Seq Data Series

Zhao

Cao

Gong

et al. 2014

BioMed Research International

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RNA-Seq is emerging as an increasingly important tool in biological research, and it provides the most direct evidence of the relationship between the physiological state and molecular changes in cells. A large amount of RNA-Seq data across diverse experimental conditions have been generated and deposited in public databases. However, most developed approaches for coexpression analyses focus on the coexpression pattern mining of the transcriptome, thereby ignoring the magnitude of gene differences in one pattern. Furthermore, the functional relationships of genes in one pattern, and notably among patterns, were not always recognized. In this study, we developed an integrated strategy to identify differential coexpression patterns of genes and probed the functional mechanisms of the modules. Two real datasets were used to validate the method and allow comparisons with other methods. One of the datasets was selected to illustrate the flow of a typical analysis. In summary, we present an approach to robustly detect coexpression patterns in transcriptomes and to stratify patterns according to their relative differences. Furthermore, a global relationship between patterns and biological functions was constructed. In addition, a freely accessible web toolkit “coexpression pattern mining and GO functional analysis” (COGO) was developed.

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Section: Application and Resultssupporting

confidence: 93%

Stratification of Gene Coexpression Patterns and GO Function Mining for a RNA-Seq Data Series

Zhao

Cao

Gong

et al. 2014

BioMed Research International

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“…Pineal AANAT band intensity in each sample was normalized by the intensity of β-actin (internal standard) and expressed as relative densitometric units (ratio of AANAT band intensity to β-actin) (Seth & Maitra, 2010a, 2010b. Mean ± SE values of the relative densitometric data of immunoblots for AANAT as well as the pineal titers of melatonin and calcium in different groups were analyzed by one-way analysis of variance (ANOVA), where F values indicated significance, and means were compared by a post hoc multiple range test, with p < .05 taken as threshold of statistical significance (Zar, 1974).…”

Section: Discussionmentioning

confidence: 99%

“…The isolated pineals were incubated in RPMI 1640 medium in a chamber at 20°C ± 1°C in the presence of 95% O 2 and 5% CO 2 . Our recent studies revealed that melatonin synthesis in the carp pineal reaches its diurnal peak 6 h after the onset of darkness in a 12L:12D cycle (Seth & Maitra, 2010a), but such peak does not occur in presence of light (Seth & Maitra, 2010b). Moreover, a schedule of 6-h incubation is considered ideal for the measurement of any change in the profile of fish pineal AANAT (Thibault et al, 1993).…”

Section: Pineal Organ Culture and Experimental Paradigmmentioning

confidence: 97%

“…This was followed by incubation with the respective secondary antibody-(i) goat antirabbit IgG conjugated with alkaline phosphatase for AANAT or (ii) goat antimouse IgG conjugated with alkaline phosphatase for β-actin-at a dilution of 1:500 for 2 h at room temperature (22°C). The antibody used in this study was the same as previously used (Seth & Maitra, 2010a, 2010b to identify AANAT in the carp pineal. Nonetheless, the specificity of AANAT antibody was determined by a preabsorption test.…”

Section: Western Blot Analysismentioning

confidence: 99%

“…Immunolabelling was done by BCIP/NBT following the manufacturer's instructions. Individual band intensity of the Western blots was quantified by densitometry analysis using Image J software (Seth & Maitra, 2010a, 2010b.…”

Section: Western Blot Analysismentioning

confidence: 99%

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Neural Regulation of Dark-Induced Abundance of ArylalkylamineN-Acetyltransferase (AANAT) and Melatonin in the Carp (Catla catla) Pineal: AnIn VitroStudy

Seth

Maitra

2011

Chronobiology International

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In all the vertebrates, synthesis of melatonin and its rhythm-generating enzyme arylalkylamine N-acetyltransferase (AANAT) reaches its peak in the pineal during the night in a daily light-dark cycle, but the role of different neuronal signals in their regulation were unknown for any fish. Hence, the authors used specific agonist and antagonists of receptors for different neuronal signals and regulators of intracellular calcium (Ca(2+)) and adenosine 3',5'-cyclic monophosphate (cAMP) in vitro to study their effects on the abundance of AANAT and titer of melatonin in the carp (Catla catla) pineal. Western blot analysis followed by quantitative analysis of respective immunoblot data for AANAT protein, radioimmunoassay of melatonin, and spectrophotometric analysis of Ca(2+) in the pineal revealed stimulatory effects of both adrenergic (α(1) and β(1)) and dopaminergic (D(1)) agonists and cholinergic (both nicotinic and muscarinic) antagonists, inhibition by both adrenergic and dopaminergic antagonists and cholinergic agonists, but independent of the influence of any agonists or antagonists of α(2)-adrenergic receptors. Band intensity of AANAT and concentration of melatonin in the pineal were also enhanced by the intracellular calcium-releasing agent, activators of both calcium channel and adenylate cyclase, and phophodiesterase inhibitor, but suppressed by inhibitor of calcium channel and adenylate cyclase as well as activator of phophodiesterase. Moreover, an inhibitory effect of light on the pineal AANAT and melatonin was blocked by both cAMP and proteasomal proteolysis inhibitor MG132. Collectively, these data suggest that dark-induced abundance of AANAT and melatonin synthesis in the carp pineal are a multineuronal function, in which both adrenergic (α(1) and β(1), but not α(2)) and dopaminergic signals are stimulatory, whereas cholinergic signals are inhibitory. This study also provides indications, though arguably not conclusive evidence, that in either case the neuronal mechanisms follow a signal-transduction pathway in which Ca(2+) and cAMP may act as the intracellular messengers. It also appears that proteasomal proteolysis is a conserved event in the regulation of AANAT activity in vertebrates.

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Annual patterns of ocular melatonin level in the female grass puffer, Takifugu alboplumbeus: possible involvement in seasonal reproductive response

Kim

Hur

Hyeon

et al. 2020

Fish Physiol Biochem

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IMPORTANCE OF LIGHT IN TEMPORAL ORGANIZATION OF PHOTORECEPTOR PROTEINS AND MELATONIN-PRODUCING SYSTEM IN THE PINEAL OF CARPCATLA CATLA

Cited by 19 publications

References 60 publications

Stratification of Gene Coexpression Patterns and GO Function Mining for a RNA-Seq Data Series

Stratification of Gene Coexpression Patterns and GO Function Mining for a RNA-Seq Data Series

Neural Regulation of Dark-Induced Abundance of ArylalkylamineN-Acetyltransferase (AANAT) and Melatonin in the Carp (Catla catla) Pineal: AnIn VitroStudy

Annual patterns of ocular melatonin level in the female grass puffer, Takifugu alboplumbeus: possible involvement in seasonal reproductive response

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