Importance of laccase in vegetative growth of pleurotus Florida

Das, Nirmalendu; Sengupta, S. K.; Mukherjee, M.

doi:10.1128/aem.63.10.4120-4122.1997

Cited by 69 publications

(40 citation statements)

References 20 publications

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“…Enzyme production and purification: The mycelial strain of P. florida ITCC 3308 was obtained from Society for Rural Industrialisation, Ranchi, India and maintained in PDA media (DAS and MUKHERJEE 1999). The mushroom was grown in potato-dextrose-yeast extract (PDY) medium (DAS et al 1997) for 5 days. The culture supernatant was obtained by centrifugation (21,000 K g, 30 min).…”

Section: Methodsmentioning

confidence: 99%

“…Native PAGE and SDS -PAGE were performed according to method of GABRIEL (1971) and LAEMMLI (1970), respectively. Activity staining after Native PAGE was done as reported earlier (DAS et al 1997). Isoelectric focusing (IEF) in the pH range 2.5 to 5.0 was performed on SE 220 mighty small tube gel adapter kit (HOEFER Scientific Instrument, USA) following the manufacturer's instruction.…”

Section: Enzyme Electrophoresis and Isoelectric Focusingmentioning

confidence: 99%

“…(MUNOZ et al 1997, PALMIERI et al 1993, SANNIA et al 1986, RESCIGNO et al 1993, YOUN et al 1995 but role of these enzyme in producer organisms has not yet been reported. Pleurotus florida produces two extracellular laccase isozymes (L 1 and L 2 ) and involvement of L 2 in the regulation of mycelial growth and yield of the mushroom has been suggested (DAS et al 1997). In the present paper, we report the purification and characterization of L 2 enzyme from culture filtrate of P. florida.…”

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confidence: 99%

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Purification and characterization of a growth-regulating laccase from Pleurotus florida

Das¹,

Chakraborty²,

Mukherjee³

2001

J. Basic Microbiol.

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Pleurotus florida produces two laccase enzymes (L1 and L2) within which L2 is associated with the vegetative growth of the fungus. In the present investigation the L2 has been purified to homogeneity and characterized. The molecular mass of the enzyme has been determined to be 73 kDa and 70 kDa by gel filtration chromatography and SDS‐PAGE, respectively. The purified enzyme shows a pI value of 4.2. The optimum reaction temperature is 50 °C. Spectroscopic analysis reveals that L2 has two copper atoms, a type I copper and a type II copper. The Km and some other kinetic parameters of L2 has been determined.

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Section: Methodsmentioning

confidence: 99%

Section: Enzyme Electrophoresis and Isoelectric Focusingmentioning

confidence: 99%

mentioning

confidence: 99%

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Purification and characterization of a growth-regulating laccase from Pleurotus florida

Das¹,

Chakraborty²,

Mukherjee³

2001

J. Basic Microbiol.

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“…Similarly, laccase production by P. ostreatus was also affected by agitation speed, agitation above 150 rev min )1 leading to lysis of the cells and resulting in low yield of laccase. Das et al (1997) predicted a direct relation between mycelial growth and laccase production in P. ostreatus under shake flask fermentations. This is supported by the low yield of laccase obtained in this study at 100 rev min )1 agitation.…”

Section: Discussionmentioning

confidence: 93%

“…Laccase production in the bench top bioreactor was measured using o-dianisidine as the substrate following the method of Das et al (1997). For antioxidant assay, laccase activity was estimated at 37°C in presence of 1AE5 mmol l )1 ABTS in phosphate buffer (10 mmol l )1 , pH 7AE4).…”

Section: Enzyme Assaymentioning

confidence: 99%

Study of laccase production byPleurotus ostreatusin a 5 l bioreactor and application of the enzyme to determine the antioxidant concentration of human plasma

Mazumder

Bose

Bandopadhyay

et al. 2008

Letters in Applied Microbiology

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Aims: To achieve high laccase production from Pleurotus ostreatus in a bench top bioreactor and to utilize the enzyme for determination of the total antioxidant concentration (TAC) of human plasma. Methods and Results: Laccase production by P. ostreatus studied in a benchtop bioreactor was as high as, 874·0 U ml−1 in presence of copper sulfate. The enzyme was used to replace metmyoglobin and hydrogen peroxide for the estimation of TAC in human plasma. The trolox equivalent antioxidant concentrations determined by the laccase‐based method and metmyoglobin method ranged from 1·63 ± 0·011 to 1·80 ± 0·006 mmol l−1 and from 1·41 ± 0·004 to 1·51 ± 0·008 mmol l−1 plasma, respectively. Conclusions: Pleurotus ostreatus produced high amount of extracellular laccase in a benchtop bioreactor. The enzyme can be used to assay TAC of blood plasma without the interference encountered with the hydrogen peroxide and metmyoglobin mediated assay method. Significance and Impact of the Study: Laccase production by P. ostreatus obtained in this study was the highest among all reported laccase producing white‐rot fungi. Moreover, an accurate laccase‐based assay method was developed for detection of TAC in human plasma.

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Role of potato extract in extracellular laccase production of Pleurotus florida

Das

Chakraborty

Mukherjee

1999

J. Basic Microbiol.

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Pleurotus florida produces extracellular laccase when grown on potato-dextrose medium but not in the other minimal media containing only dextrose. When potato extract has been fractionated in G-25 column two types of fractions were obtained with respect to iodine colouration. Fractions of the first peak (P1) gives blue colouration with iodine indicating the presence of starch whereas the fractions of the other region (P2) decolourise the iodine colour indicating presence of one or more reducing compound(s). The P2 fractions give red colour after reacting with diazonized sulphanilic acid suggesting phenolic nature of the compound(s). Addition of fractions of either P1 or P2 in the minimal media can not support the laccase production in P. florida but combination of the two fractions is sufficient to induce laccase production. Similarly minimal media containing starch from the other two sources can stimulate the laccase production in P. florida only in presence of P2 fraction. On the basis of these observation we propose that phenolic compound(s) present in potato extract is capable of inducing laccase synthesis in P. florida.

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Importance of laccase in vegetative growth of pleurotus Florida

Cited by 69 publications

References 20 publications

Purification and characterization of a growth-regulating laccase from Pleurotus florida

Purification and characterization of a growth-regulating laccase from Pleurotus florida

Study of laccase production byPleurotus ostreatusin a 5 l bioreactor and application of the enzyme to determine the antioxidant concentration of human plasma

Role of potato extract in extracellular laccase production of Pleurotus florida

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