Importance of instrumentation for fast liquid chromatography in pharmaceutical analysis

Fekete, Szabolcs; Köhler, Isabelle; Rudaz, Serge; Guillarme, Davy

doi:10.1016/j.jpba.2013.03.012

Cited by 115 publications

(87 citation statements)

References 96 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…80% of the one achieved with fully porous sub-2 μm particles has been reported with sub-3 μm core-shell particles but with a 2 to 3-times lower backpressure compared to sub 2 μm totally porous particles. [16][17][18][19] To achieve high throughput analysis, we used a 100 × 2.1 mm i.d. column packed with 2.7 μm core-shell particles.…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

Determination of Pesticides in Grape Juices by QuEChERS and Liquid Chromatography-Tandem Mass Spectrometry

Souza¹,

Souza²,

Borges³

2016

Journal of the Brazilian Chemical Society

View full text Add to dashboard Cite

In this study, we describe a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying 25 pesticides in grape juice. This method was optimized and validated according with INMETRO (Instituto Nacional de Metrologia Qualidade e Tecnologia) for the simultaneous determination of pesticide, which are widely used in grape culture in Brazil. The method was based on the QuEChERS (quick, easy, cheap, effective, rugged and safe) methodology. We used AOAC Official Method 2007.01 and Standard Method EN 15662 for sample preparation, both methods presented statistically equivalent recoveries and coefficients of variation. At 0.005 and 2 mg L -1 , fortified blank grape juices had average recoveries of 101 and 112% and average coefficients of variation of 16 and 11%, respectively. The repeatability was tested in three concentrations 0.005, 0.1 and 2 mg L -1 , affording coefficients of variation 16.4, 20.7 and 10.8%, respectively.

show abstract

Section: Chromatographic Conditionsmentioning

confidence: 99%

Determination of Pesticides in Grape Juices by QuEChERS and Liquid Chromatography-Tandem Mass Spectrometry

Souza¹,

Souza²,

Borges³

2016

Journal of the Brazilian Chemical Society

View full text Add to dashboard Cite

show abstract

“…Extra-column band broadening effects are taking place in the "external" instrument (extra-column) volumes, including the injector system, the connector tubing, and the detector cell [10]. An additional contribution is related to time-based effects, namely on the time constant and sampling rate of the detector.…”

Section: Extra-column Band Broadeningmentioning

confidence: 99%

“…Extra-column volume is a physical characteristic of the instrument and can be easily evaluated in terms of volume unit (e.g., L). On the other hand, the extra-column peak variance accounts for the sample dispersion before and after the column [10]. Extra-column dispersion (or variance) can be calculated in time square or volume square units.…”

Section: Extra-column Band Broadeningmentioning

confidence: 99%

“…Today, there is a large choice of column packed with fully porous sub-2 m particles which are compatible with pressures up to 1,200 bar [3,10,11,188]. These stationary phases consist of (i) hybrid silica material prepared from two monomers, i.e., tetraethoxysilane and bis(triethoxysilyl) ethane which incorporates ethylene bridges (the high degree of cross-linking ensures strong mechanical and hydrolytic stability) [189], or (ii) classical silica simply packed under much higher pressure conditions.…”

Section: Upper Pressure Limit Of Current Instrumentmentioning

confidence: 99%

“…It is also important to notice that the kinetic performance evaluation of state-of-the-art LC phases becomes a topic of high relevance for the scientific community in the last ten years [7][8][9]. Together with the evolution of chromatographic supports, the systems have also been drastically improved to be compatible with the most recent stationary phases [10][11][12]. Compared to old generation HPLC instruments, the current systems on the market possess an extended pressure range (up to 1000-1300 bar for most of them).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Fekete¹,

Perrenoud²,

Guillarme

2014

CCHG

Self Cite

View full text Add to dashboard Cite

Abstract:The current trend in high performance liquid chromatography (HPLC) tends toward the achievement of higher separation efficiency and shorter analysis time. Indeed, better performance in LC has become increasingly important in recent years mainly driven by the challenges of either analyzing more complex samples or increasing the numbers of samples per time unit. In the recent development of particle technology, the use of fully porous sub-2 m particles and sub-3 m shell particles have received considerable attention. Beside packed columns, the new generation of silica-based monolithic columns also offers very high separation power. However, to take full advantage of these innovative phases, the chromatographic system has also to be drastically optimized in terms of upper pressure limit and system volume.This revolution in column technology now spreads and covers several modes of liquid chromatography such as reversedphase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC), or even supercritical fluid chromatography (SFC). The HILIC and SFC, which can be considered as alternative modes of chromatography, could also be useful to extend the applicability of chromatography towards the analysis of very hydrophilic and lipophilic compounds, respectively.The present review gives an insight about the theory behind the success of current column technology and presents a summary of latest applications, using various modes of one-dimensional chromatography (RPLC, HILIC, SFC). This paper also shows that theoretically expected column efficiency could sometimes be compromised in practical work especially in the case of narrow bore columns.

show abstract

HPLC/UHPLCInstrumentation and Trends

2019

HPLC and UHPLC for Practicing Scientists

View full text Add to dashboard Cite

Importance of instrumentation for fast liquid chromatography in pharmaceutical analysis

Cited by 115 publications

References 96 publications

Determination of Pesticides in Grape Juices by QuEChERS and Liquid Chromatography-Tandem Mass Spectrometry

Determination of Pesticides in Grape Juices by QuEChERS and Liquid Chromatography-Tandem Mass Spectrometry

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

HPLC/UHPLCInstrumentation and Trends

Contact Info

Product

Resources

About