Importance of bile in regulation of intraluminal proteolytic enzyme activities in the rat

Green, G.M.; Nasset, E. S.

doi:10.1016/0016-5085(80)90247-4

Cited by 99 publications

(21 citation statements)

References 16 publications

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“…F: in contrast, immunoreactivity for ␣-SMA was rarely noted except in the vessel walls in the control group. Original magnification: ϫ100 in A-D and F and ϫ200 in E. 16.4 Ϯ 3.5 cmH 2 O). In addition, surgical decompression of the pancreatic duct (12,32,35), endoscopic ductal drainage (11,22,38), or removal of intraductal stones by extracorporeal shock wave lithotripsy (29,36,40) have improved the clinical outcome of patients with CP.…”

Section: Discussionmentioning

confidence: 96%

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A new model of chronic pancreatitis in rats

Yamamoto

Otani²,

Ōtsuki³

2006

American Journal of Physiology-Gastrointestinal and Liver Physi

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This study was designed to examine whether continuous pancreatic ductal hypertension (PDH) plays an important role in the onset and development of chronic pancreatitis (CP). Pancreatic, biliary, and duodenal cannulas were implanted in male Wistar rats. PDH was induced by vertically raising the free end of the pancreatic duct cannula to exert a hydrostatic pressure and maintained for 2 wk. PDH was gradually increased, but when the pancreatic juice (PJ) flow was interrupted, PDH was decreased to restore PJ flow. The induction of PDH resulted in a marked reduction of amylase activity in PJ and an increase in serum amylase activity. At 2 wk after persistent PDH, pancreatic exocrine function was markedly decreased in response to a bolus injection of secretin (100 pmol/kg) compared with the control group. Histological examination revealed interlobular as well as intralobular fibrosis in the form of nodular pancreatitis at 2 wk after the induction of PDH. Immunohistochemistry revealed the expression of fibronectin and collagen types I and III. Quantitative real-time RT-PCR showed an increase in transforming growth factor-beta(1) mRNA expression in the pancreas during PDH. The present results suggest that PDH plays an important role in the onset and development of CP. Furthermore, our animal model seems useful for investigating the mechanisms of CP in rats.

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Section: Discussionmentioning

confidence: 96%

“…After the rats' abdomen was closed, rats were prepared with a jugular vein cannula and then placed into a modified Bollman-type restraint cage. Surgical procedures were conducted according to the methods of Green et al (16). During the recovery and experimental periods, rats had full access to food and water ad libitum.…”

Section: Methodsmentioning

confidence: 99%

A new model of chronic pancreatitis in rats

Yamamoto

Otani²,

Ōtsuki³

2006

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…As an explanation of the inhibitory effect of luminal bile or bile salts, it has been proposed that bile protects luminal proteases from autodigestion and that luminal proteases inhibited CCK release and pancreatic secretion in rats * This value significantly higher than the value produced by saline injection by Wilcoxon rank sum test. (5). Gomez et al (25) reported that administration of cholestyramine increased CCK release and pancreatic secretion stimulated by intraduodenal administration of amino acid in dogs and humans.…”

Section: Discussionmentioning

confidence: 96%

Effect of Taurocholate on CCK Release and Pancreatic Secretion Produced by Two CCK-Releasing Peptides in Conscious Rats

Miyasaka¹,

Funakoshi²,

Shikado³

et al. 1992

Pancreas

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The role of luminal bile salts (taurocholate) in regulation of rat pancreatic secretion was examined by studies on the effects of luminal stimulants on the pancreas during infusion of various concentrations of taurocholate into the duodenum of conscious rats. Rats with external bile and pancreatic fistulae were used. For 24 h before the experiment, pancreatic juice was excluded from the intestine but bile was continuously returned to the duodenum. From the beginning of the experiment, 8-200 mM of taurocholate was infused at a rate of 1 ml/h instead of returning the bile. Pancreatic juice was collected for a 2-h period and then 2 pg of pancreatic secretory trypsin inhibitor-61 (PSTI-61) ( =monitor peptide) or partially purified putative CCK-releasing peptide from rat intestine (intestinal CCK-RP) was injected into the duodenum (1 mllrnin). Continuous infusion of taurocholate maintained a constant rate of pancreatic secretion, except at a concentration of 8 mM, which resulted in a slight increase in pancreatic secretion. Both PSTI-61 and intestinal CCK-RP significantly increased pancreatic secretions during infusion of 20 or 40 mM taurocholate, but had no significant effect during infusion of 80 or 200 mM taurocholate. Therefore, higher concentrations of taurocholate in the intestine prevented the stimulatory effects of luminal stimulants, probably by preventing the latter from reaching CCK cells.

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“…This was not due to the lack of pancreatic enzymes, since PJ was reintroduced into the duodenum and its protein content and trypsin activity were high. Under these conditions, pancreatic proteases could be autodigested in the intestinal lumen, as suggested by Green and Nasset (1980), leading to a decreased food protein cleavage and increased protein loss from the small intestine. Long lasting diversion of both bile and PJ caused an elevated PJ outflow within the time of the observations, but did not influence either protein outflow or trypsin activity.…”

Section: Discussionmentioning

confidence: 96%

“…In pigs, there is a controversy concerning the existence of feedback regulation. Bile has been known to affect feedback regulation in animal species other than pigs and in humans, but the mechanism remains unclear (Green and Nasset, 1980;Houe et al, 1997). Some researchers suggested that bile acids may stabilize luminal protease activity resulting in inhibition of CCK release, and others are of the opinion that the inhibition of plasma CCK is achieved due to enhanced somatostatin release by the bile (Koop et al, 1996).…”

Section: Introductionmentioning

confidence: 98%