Importance of B4 Medium in Determining Organomineralization Potential of Bacterial Environmental Isolates

Marvasi, Massimiliano; Gallagher, Kimberley L.; Martinez, Lilliam Casillas; Pagan, William C. Molina; Santiago, Ronald E. Rodrí­guez; Vega, Gloria Castilloveití­a; Visscher, Pieter T.

doi:10.1080/01490451.2011.636145

Cited by 44 publications

(37 citation statements)

References 54 publications

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“…This observation is not surprising since microorganisms can change the microenvironmental conditions with their metabolism (Marvasi et al 2010;Marvasi et al 2012;Dupraz et al 2009b;Dupraz et al 2008).…”

Section: Discussionmentioning

confidence: 96%

“…For these applied subjects, the need for a laboratory in vitro model system is required and also the size of the crystal is important. Previous studies have already used Bacillus subtilis 168 as a type strain to study calcite precipitation on biofilms by using the maximum medium B4 rich in calcium (Barabesi et al 2007;Marvasi et al 2010;Marvasi et al 2012;Boquet et al 1973). Calcite crystals are obtained by streaking B. subtilis 168 strain on B4 medium and calcite crystals are visible on B4 within 7 days at 37˚C.…”

Section: Introductionmentioning

confidence: 99%

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Factors Affecting Formation of Large Calcite Crystals (≥1 mm) in Bacillus subtilis 168 Biofilm

Perito

Casillas

Marvasi

2018

Geomicrobiology Journal

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Factors Affecting Formation of Large Calcite Crystals (≥1 mm) in Bacillus subtilis 168 Biofilm

Perito

Casillas

Marvasi

2018

Geomicrobiology Journal

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“…For coupled calcium-carbon sources, like ACE (Fig. 4d, S5), the mineralization mainly occurred over cellular structures such as cell wall and EPS (Marvasi et al 2012;Dhami et al 2013). In that sources, critical Ca 2+ concentration could be accumulated in cell wall because the e ux pump and speci c channels (Saier et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Novel method to achieve crystallinity of calcite by Bacillus subtilis in coupled and non-coupled calcium-carbon sources

Ferral-Pérez

Galicia

Alvarado-Tenorio

et al. 2020

Preprint

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Bacteria mineralization is a promising biotechnological approach to apply in biomaterials development. In this investigation, we demonstrate that Bacillus subtilis 168 induces and influences CaCO3 composites precipitation. Crystals were formed in calcium-carbon non-coupled (glycerol + CaCl2, GLY; or glucose + CaCl2, GLC) and coupled (calcium lactate, LAC; or calcium acetate, ACE) agar-sources, only maintaining the same Ca2+ concentration. The mineralized colonies showed variations in morphology, size, and crystallinity form properties. The crystals presented spherulitic growth in all conditions, and botryoidal shapes in GLC one. Birefringence and diffraction patterns confirmed that all biogenic carbonate crystals (BCC) were organized as calcite. The CaCO3 in BCC was organized as calcite, amorphous calcium carbon (ACC) and organic matter (OM) of biofilm; all of them with relative abundance related to bacteria growth condition. BCC-GLY presented greatest OM composition, while BCC-ACE highest CaCO3 content. Nucleation mechanism and OM content impacted in BCC crystallinity.

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“…This indicates that the increase in pH in the medium through the decomposition of urea played a significant role in Ca-carbonate precipitation. [23]. When 0.1 M of Ca-acetate was used, the maximum yield was about 11.5 g/L and the production amount was also proportional to the concentration of Ca ion.…”

Section: Factors Influencing Maximum Ca-carbonate Precipitationmentioning

confidence: 91%

“…When the concentrations of Ca-acetate added were varied from 0 to 0.5 M, the highest amount of calcite, 22.8 g/L, was produced when 0.3 M Ca-acetate was injected into SMP solution of WD-1 (Figure 5b). This indicates that urea is degraded by microorganisms, which increases the concentration of bicarbonate and the pH value (pH 9) in the medium, and that these geochemical changes in the medium provide a favorable environment to lead to the carbonate precipitation of Ca ions [8,22,23]. In general, Ca-carbonate precipitation requires supersaturation with a saturation index (SI = [Ca 2+ ] × [CO 3 2− ]/Ksp) higher than 1.…”

Section: Factors Influencing Maximum Ca-carbonate Precipitationmentioning

confidence: 99%

Microbially Induced Carbonate Precipitation Using Microorganisms Enriched from Calcareous Materials in Marine Environments and Their Metabolites

Kim

Roh

2019

Minerals

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Microbially induced Ca-carbonate precipitation (MICP) in general, refers to a process in which the urease secreted by microbes hydrolyzes urea to ammonium and carbon dioxide. The main objectives of this study were to identify the environmental factors (e.g., microbial growth, cell/metabolite presences, and calcium sources) that control Ca-carbonate formation and to investigate the mineralogical characteristics of the Ca-carbonate precipitated using ureolytic microorganisms cultured in marine environments. The two types of carbonate-forming microorganisms (CFMs), mixed cultures hydrolyzing urea, were enriched from calcareous materials in marine environments. The experiments using a CFM, Sporosarcina pasteurii, was also used for comparison. All the microbes were cultured aerobically in D-1 growth media that included urea. To investigate the effect of microbial growth states on Ca-carbonate precipitation, Ca-acetate was injected into the media before (i.e., lag phase) and after (i.e., stationary phase) microbial growth, and into the soluble microbial products (SMP) solution, respectively. XRD, FT-IR, and SEM-EDS analyses were used for mineralogical characterization of the precipitated Ca-carbonates. Results indicated that the Ca-carbonates, vaterite and/or calcite, precipitated under all the experimental conditions. The fastest precipitation of Ca-carbonates occurred in the SMP solution and formed calcite (size = 5–15 μm). When the concentrations of added Ca-acetate were varied from 0 to 0.5 M, the highest amounts of calcite, 22.8 g/L, were produced when 0.3 M Ca-acetate was injected. Therefore, the environmental factors (e.g., microbial growth, cell/metabolite presences, and calcium sources) could have an effect the rate of formation of Ca-carbonate and the types of carbonate minerals formed. Moreover, the use of cell-free SMP solution is expected to be applicable to Ca-carbonate precipitation in an environment where microbial growth is unfavorable.

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Importance of B4 Medium in Determining Organomineralization Potential of Bacterial Environmental Isolates

Cited by 44 publications

References 54 publications

Factors Affecting Formation of Large Calcite Crystals (≥1 mm) in Bacillus subtilis 168 Biofilm

Factors Affecting Formation of Large Calcite Crystals (≥1 mm) in Bacillus subtilis 168 Biofilm

Novel method to achieve crystallinity of calcite by Bacillus subtilis in coupled and non-coupled calcium-carbon sources

Microbially Induced Carbonate Precipitation Using Microorganisms Enriched from Calcareous Materials in Marine Environments and Their Metabolites

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Importance of B4 Medium in Determining Organomineralization Potential of Bacterial Environmental Isolates

Cited by 44 publications

References 54 publications

Factors Affecting Formation of Large Calcite Crystals (≥1 mm) in Bacillus subtilis 168 Biofilm

Factors Affecting Formation of Large Calcite Crystals (≥1 mm) in Bacillus subtilis 168 Biofilm

Novel method to achieve crystallinity of calcite by Bacillus subtilis in&nbsp;coupled and non-coupled calcium-carbon sources

Microbially Induced Carbonate Precipitation Using Microorganisms Enriched from Calcareous Materials in Marine Environments and Their Metabolites

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Novel method to achieve crystallinity of calcite by Bacillus subtilis in coupled and non-coupled calcium-carbon sources