Importance of antigen-lymphocyte interaction in establishment of cellular immunity

Naito, Mitsuko; Seno, Satimaru

doi:10.1016/0309-1651(81)90187-9

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…After 2 days, the medium was removed. Poly-L-lysine (50 µg/ml) was added and the cells were incubated for 2 h. 21) The cells were washed and adjusted to 1.3×10 8…”

mentioning

confidence: 99%

Autologous Fixed Tumor Vaccine: A Formulation with Cytokine‐microparticles for Protective Immunity against Recurrence of Human Hepatocellular Carcinoma

Peng

Liu

Kuang

et al. 2002

Japanese Journal of Cancer Research

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We developed a tumor vaccine consisting of fixed hepatocellular carcinoma (HCC) cells/tissue fragments, biodegradable microparticles encapsulating granulocyte-macrophage-colony stimulating factor and interleukin-2, and an adjuvant. The vaccine protected 33% of syngeneic mice from HCC cell challenge. The vaccine containing human autologous HCC fragments showed essentially no adverse effect in a phase I/IIa clinical trial and 8/12 patients developed a delayed-type hypersensitivity (DTH) response against the fragments. Although 2 of 4 DTH-response-negative patients had recurrence after curative resection, the DTH-response-positive patients had no recurrence. The time before the first recurrence in the vaccinated patients was significantly longer than that in 24 historical control patients operated in the same department (P < < < <0.05). This formulation is a promising candidate to prevent recurrence of human HCC.

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“…After 2 days, the medium was removed. Poly-L-lysine (50 µg/ml) was added and the cells were incubated for 2 h. 21) The cells were washed and adjusted to 1.3×10 8…”

mentioning

confidence: 99%

Autologous Fixed Tumor Vaccine: A Formulation with Cytokine‐microparticles for Protective Immunity against Recurrence of Human Hepatocellular Carcinoma

Peng

Liu

Kuang

et al. 2002

Japanese Journal of Cancer Research

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“…Moreover, the phagocytized particles used in our experiment were GRC. A GRC can not attach to lymphocytes because of its negative surface charge (20,26). Therefore, if only this one characteristic is considered, K-23-M might be considered a monocytic cell line.…”

Section: Discussionmentioning

confidence: 99%

A lymphoblastoid cell line with high phagocytic activity established from peripheral blood of a patient with chronic myelocytic leukemia in blast crisis.

Mitani

Hyodoh

Yawata

et al. 1983

Cell Struct. Funct.

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ABSTRACT. A human hematopoietic cell line (K-23-M) was established from a patient with chronic myelocytic leukemia in blast crisis. Morphologically, the cultured cells were lymphoblastoid cells that produced 1gA and were Epstein-Barr viral nuclear antigen positive. But they showed high phagocytic activity to glutaraldehyde-treated sheep red cells and had properties of a monocyte or macrophage that included surface Fc receptors, a-naphthyL butyrate esterase positivity blocked by NaF, migration in soft agar and the ability to attach to a glass surface. Lysozyme secretion was absent, and chromosomes were diploid and Ph1 negative. This cell line is unique in that it has strong phagocytic activity. Its existence shows that lymphoblastoid cell line may be a more important cell line for the study of human hematopoietic cells than previously has been believed.

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Protective antitumor immunity induced by fixed tumor cells in combination with adjuvant in a murine hepatoma model

Huang

Ohno

2003

Cancer Letters

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Importance of antigen-lymphocyte interaction in establishment of cellular immunity

Cited by 3 publications

References 13 publications

Autologous Fixed Tumor Vaccine: A Formulation with Cytokine‐microparticles for Protective Immunity against Recurrence of Human Hepatocellular Carcinoma

Autologous Fixed Tumor Vaccine: A Formulation with Cytokine‐microparticles for Protective Immunity against Recurrence of Human Hepatocellular Carcinoma

A lymphoblastoid cell line with high phagocytic activity established from peripheral blood of a patient with chronic myelocytic leukemia in blast crisis.

Protective antitumor immunity induced by fixed tumor cells in combination with adjuvant in a murine hepatoma model

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