Importance of adhesins in the recurrence of pharyngeal infections caused by Streptococcus pyogenes

Wozniak, Aniela; Scioscia, Natalia; Geoffroy, Enrique; Ponce, Iván; García, Patricia

doi:10.1099/jmm.0.000464

Cited by 6 publications

(5 citation statements)

References 38 publications

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“…The only study among these with a sufficient number of emm3 isolates, found a more homogenous distribution of biofilm forming capacity within this emm type compared to our data (Baldassarri et al, 2006). Other studies addressing the relationship between emm type and biofilm formation includes no or just a few emm1, emm3, and/or emm28 isolates (Lembke et al, 2006;Thenmozhi et al, 2011;Wozniak et al, 2017), making a comparison to our results unreliable.…”

Section: Discussioncontrasting

confidence: 86%

“…M protein, encoded by the emm gene, has an important role in S. pyogenes biofilm formation (Cho and Caparon, 2005;Courtney et al, 2009). However, there is conflicting evidence for a potential association between emm type and biofilm formation (Baldassarri et al, 2006;Koller et al, 2010;Ogawa et al, 2011;Wozniak et al, 2017). Other surface associated molecules known to be involved in S. pyogenes biofilm formation include pili (Manetti et al, 2007), streptococcal collagen like protein 1 (Scl1) (Oliver-Kozup et al, 2011) and hyaluronic acid capsule (Cho and Caparon, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Consistent Biofilm Formation by Streptococcus pyogenes emm 1 Isolated From Patients With Necrotizing Soft Tissue Infections

et al. 2022

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ObjectivesBiofilm formation has been demonstrated in muscle and soft tissue samples from patients with necrotizing soft tissue infection (NSTI) caused by Streptococcus pyogenes, but the clinical importance of this observation is not clear. Although M-protein has been shown to be important for in vitro biofilm formation in S. pyogenes, the evidence for an association between emm type and biofilm forming capacity is conflicting. Here we characterize the biofilm forming capacity in a collection of S. pyogenes isolates causing NSTI, and relate this to emm type of the isolates and clinical characteristics of the patients.MethodsBacterial isolates and clinical data were obtained from NSTI patients enrolled in a multicenter prospective observational study. Biofilm forming capacity was determined using a microtiter plate assay.ResultsAmong 57 cases, the three most frequently encountered emm types were emm1 (n = 22), emm3 (n = 13), and emm28 (n = 7). The distribution of biofilm forming capacity in emm1 was qualitatively (narrow-ranged normal distribution) and quantitatively (21/22 isolates in the intermediate range) different from other emm types (wide ranged, multimodal distribution with 5/35 isolates in the same range as emm1). There were no significant associations between biofilm forming capacity and clinical characteristics of the patients.ConclusionsThe biofilm forming capacity of emm1 isolates was uniform and differed significantly from other emm types. The impact of biofilm formation in NSTI caused by S. pyogenes on clinical outcomes remains uncertain.

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Section: Discussioncontrasting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Consistent Biofilm Formation by Streptococcus pyogenes emm 1 Isolated From Patients With Necrotizing Soft Tissue Infections

et al. 2022

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“…For preparation of the infective inoculum, an erythromycin‐resistant S. pyogenes clinical isolate of each M type that had been previously reported was grown in Todd–Hewitt broth at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Protective immunity induced by an intranasal multivalent vaccine comprising 10 Lactococcus lactis strains expressing highly prevalent M‐protein antigens derived from Group A Streptococcus

Wozniak

Scioscia

García

et al. 2018

Microbiology and Immunology

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Streptococcus pyogenes (group A Streptococcus) causes diseases ranging from mild pharyngitis to severe invasive infections. The N-terminal fragment of streptococcal M protein elicits protective antibodies and is an attractive vaccine target. However, this N- terminal fragment is hypervariable: there are more than 200 different M types. In this study, an intranasal live bacterial vaccine comprising 10 strains of Lactococcus lactis, each expressing one N-terminal fragment of M protein, has been developed. Live bacterial-vectored vaccines cost less to manufacture because the processes involved are less complex than those required for production of protein subunit vaccines. Moreover, intranasal administration does not require syringes or specialized personnel. Evaluation of individual vaccine types (M1, M2, M3, M4, M6, M9, M12, M22, M28 and M77) showed that most of them protected mice against challenge with virulent S. pyogenes. All 10 strains combined in a 10-valent vaccine (M×10) induced serum and bronchoalveolar lavage IgG titers that ranged from three- to 10-fold those of unimmunized mice. After intranasal challenge with M28 streptococci, survival of M×10-immunized mice was significantly higher than that of unimmunized mice. In contrast, when mice were challenged with M75 streptococci, survival of M×10-immunized mice did not differ significantly from that of unimmunized mice. Mx-10 immunized mice had significantly less S. pyogenes in oropharyngeal washes and developed less severe disease symptoms after challenge than did unimmunized mice. Our L. lactis-based vaccine may provide an alternative solution to development of broadly protective group A streptococcal vaccines.

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“…However, the frequency of reinfection with FCT-4-type S. pyogenes was high. Since FCT-4-type S. pyogenes possesses two fibronectinbinding proteins, there may be a causal link between bacterial reinfection and adhesion molecules [14]. Moreover, the methods of bacterial genetic analysis were different between these studies and our study.…”

Section: Discussionmentioning

confidence: 66%

Analysis of Streptococcus pyogenes reinfection in pediatric patients in Japan

Minami¹,

Sakakibara²,

Watanabe³

et al. 2021

GSC Adv. Res. Rev.

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Acute pharyngitis and tonsillitis caused by Streptococcus pyogenes are common pediatric infectious diseases. Although the presence of penicillin-resistant S. pyogenes has not been confirmed in Japan, the re-isolation rate of S. pyogenes after antimicrobial treatment has been increasing. Thus, we attempted to determine whether the presence of S. pyogenes in pediatric patients treated with antimicrobial treatment was because of treatment failure or reinfection. We examined 19 patients who visited Daido Hospital between 2013 and 2017. The patient age, drug susceptibility patterns of the isolated bacteria, and random amplified polymorphic DNA (RAPD) analysis results were evaluated. The patient age ranged from 3 months to 9 years, seven patients were 5 years old. Seven patients showed consistent drug susceptible test results, and 12 patients showed inconsistent drug susceptible test results. Among these 12 patients, nine patients showed a greater decrease in drug susceptibility than the other three patients. Genetic mismatch was confirmed by RAPD analysis in all seven patients with consistent drug susceptible results. The paired S. pyogenes isolates from the same patient showed the presence of different bacteria. Our results indicate that, in many cases, re-isolation of S. pyogenes is not due to treatment failure, but due to reinfection with other clinical isolates.

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Importance of adhesins in the recurrence of pharyngeal infections caused by Streptococcus pyogenes

Abstract: Recurrence seems not to be associated with biofilm formation. However, pili and fibronectin-binding proteins could be associated with recurrence because FCT-4 isolates which harbour two fibronectin-binding proteins are more frequent among recurrent isolates.

Cited by 6 publications

References 38 publications

Consistent Biofilm Formation by Streptococcus pyogenes emm 1 Isolated From Patients With Necrotizing Soft Tissue Infections

Consistent Biofilm Formation by Streptococcus pyogenes emm 1 Isolated From Patients With Necrotizing Soft Tissue Infections

Protective immunity induced by an intranasal multivalent vaccine comprising 10 Lactococcus lactis strains expressing highly prevalent M‐protein antigens derived from Group A Streptococcus

Analysis of Streptococcus pyogenes reinfection in pediatric patients in Japan

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