Importance of a Laccase Gene (Lcc1) in the Development of Ganoderma tsugae

Abstract

In this study, a novel laccase gene (Lcc1) from Ganoderma tsugae was isolated and its functions were characterized in detail. The results showed that Lcc1 has the highest expression activity during mycelium development and fruit body maturation based on the analysis of Lcc1 RNA transcripts at different developmental stages of G. tsugae. To investigate the exact contribution of Lcc1 to mycelium and fruit body development in G. tsugae, Lcc1 transgenic strains were constructed by targeted gene replacement and over-expression approaches. The results showed that the lignin degradation rate in Lcc1 deletion mutant was much lower than the degradation efficiency of the wild-type (WT), over-expression and rescue strains. The lignin degradation activity of G. tsugae is dependent on Lcc1 and the deletion of Lcc1 exerted detrimental influences on the development of mycelium branch. Furthermore, the study uncovered that Lcc1 deletion mutants generated much shorter pale grey fruit bodies, suggesting that Lcc1 contributes directly to pigmentation and stipe elongation during fruit body development in G. tsugae. The information obtained in this study provides a novel and mechanistic insight into the specific role of Lcc1 during growth and development of G. tsugae.

Purification and characterisation of new laccase from Trametes polyzona WRF03

“…The positive amplification indicated the presence of laccase coding gene in Tp WRF03 genome. In addition, the amplicon size falls within the range of expected amplicon size (1500–1700 bp) of fungal laccases as reported by many authors that used similar primers [ [21] , [22] , [23] ]. The extracellular nature of laccase from Trametes polyzona WRF03 and the fact that the enzyme is the only ligninolytic enzyme noticeably produced by the organism facilitated the purification process.…”

“…Souza et al [ 20 ] proposed the use of designed oligonucleotide primers corresponding to these conserved sequences for PCR amplification of laccase genes. In addition, previous works carried out with primer pair designed from these conserved sequences showed positive results and were recommended for sensitive detection of laccase gene-specific sequences in white-rot fungi [ [21] , [22] , [23] ]…”

Optimization of Laccase from Ganoderma lucidum Decolorizing Remazol Brilliant Blue R and Glac1 as Main Laccase-Contributing Gene

“…The important roles of Glac1 in G. lucidum mycelium has been reported in many previous studies [54,55]. Lcc1 from G. tsugae was reported to play key roles in growth and development of G. tsugae , such as pigmentation and stipe elongation [56]. Lac1 , which was cloned from white-rot fungus Cerrena sp.…”

Relationship between fruiting body development and extracellular laccase production in the edible mushroom Flammulina velutipes

“…Thus, a considerable difference was observed between the laccase activity profiles of the FVN-1 and FVD-1 strains determined under the same fruiting body condition, especially in the early phase of low temperature shift (i.e., 15th and 16th-day cultivation), confirming the impact of temperature change on laccase activity. Several studies have explored the potential role of laccase in the morphogenesis of some basidiomycetes, including Pleurotus florida , Pleurotus ostreatus , Lentinula edodes , Hypsizygus marmoreus , and Ganoderma tsugae [ [22] , [23] , [24] , [25] , [26] ]. However, to the best of our knowledge, this is the first report showing the relationship between fruiting body development and extracellular laccase production using normal and degenerate strains of F .…”