Importance of 1,25-dihydroxyvitamin D3 and the nonadherent cells of marrow for osteoblast differentiation from rat marrow stromal cells

The Secreted Protein Thrombospondin 2 Is an Autocrine Inhibitor of Marrow Stromal Cell Proliferation

“…(16) Furthermore, TSP2-null MSCs, when cultured in vitro, show increased DNA synthesis as determined by tritiated thymidine incorporation. (16) Proliferation of MSCs in vitro is sensitive to a variety of extracellular molecules including systemic hormones such as vitamin D (17) and dexamethasone (18) and growth factors (19) such as basic FGF (bFGF), (20) platelet-derived growth factor (PDGF), (21) and the bone morphogenetic protein secreted protein (BMP) superfamily members. (22,23) The effect of extracellular matrix (ECM) proteins on MSC proliferation has not been studied as extensively.…”

Cytocentrifugation: a convenient and efficient method for seeding tendon-derived cells into monolayer cultures or 3-D tissue engineering scaffolds

“…Another possibility is the inherent selectivity of adherent rapidly proliferating cells in standard monolayer cultures. It is well known in other tissues such as bone marrow that non-adherent cells are necessary for the correct function of the mesenchymal-derived cells Friedenstein et al 1992;Rickard et al 1995). By inducing the cells to adhere by cytocentrifugation this possible culture artefact can be avoided and the behaviour of either adherent cells or adherent plus non-adherent cells observed.…”

Incidence of bone formation by whole bone marrow cell suspensions and by its stromal cell cultures injected into kidney parenchyma of Balb/c mice

“…Another reason for a poor osteogenic capability of marrow stromal cells grafted into the kidney could be the lack of added osteogenic differentiation factors to the culture medium, such as PTH, TGF-B, BMP, vitamin D. Such conditioning distinct increases differentiation [4,12,13].…”