Import of Radiation Phenomena of Electrons and Therapeutic Low-Level Laser in Regard to the Mitochondrial Energy Transfer

Wilden, Lutz; Karthein, R.

doi:10.1089/clm.1998.16.159

Cited by 93 publications

(73 citation statements)

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“…There were more TH+ cells in the MPTP-NIr groups than in the MPTP group, indicating that NIr protected cells from the MPTP insult. This finding is consistent with those in several in vitro 22,34,42,44,46 and in vivo 25,32,34,38,45 studies that have documented better cell survival after the external application of NIr. In fact, the magnitude of neuropro- tection was similar to those in previous studies using the same acute MPTP (50 mg/kg) model; whether applied externally 25,38 or internally (present study), TH+ cell numbers in the MPTP-NIr groups were not only greater than in the MPTP group, but also very similar to those in the saline control groups.…”

Section: Neuroprotection Of Dopaminergic Cells In the Snc Via Nir Aftsupporting

confidence: 92%

“…First, NIr may have stimulated mitochondria in the SNc cells directly by increasing adenosine-5ʹ-triphosphate (ATP) content, electron transfer in the respiratory chain, and activation of photoacceptors (for example, cytochrome oxidase), all leading to enhanced survival. 4,30,45 Second, NIr may have also triggered a more indirect global systemic response. 39 Several studies have reported remote, often bilateral, effects on body tissues after the local application of NIr to, for example, either skin wounds 6 or the dorsum of the body.…”

Section: Neuroprotection Of Dopaminergic Cells In the Snc Via Nir Aftmentioning

confidence: 99%

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Photobiomodulation inside the brain: a novel method of applying near-infrared light intracranially and its impact on dopaminergic cell survival in MPTP-treated mice

Moro

Massri²,

Torres

et al. 2014

JNS

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Object Previous experimental studies have documented the neuroprotection of damaged or diseased cells after applying, from outside the brain, near-infrared light (NIr) to the brain by using external light-emitting diodes (LEDs) or laser devices. In the present study, the authors describe an effective and reliable surgical method of applying to the brain, from inside the brain, NIr to the brain. They developed a novel internal surgical device that delivers the NIr to brain regions very close to target damaged or diseased cells. They suggest that this device will be useful in applying NIr within the large human brain, particularly if the target cells have a very deep location. Methods An optical fiber linked to an LED or laser device was surgically implanted into the lateral ventricle of BALB/c mice or Sprague-Dawley rats. The authors explored the feasibility of the internal device, measured the NIr signal through living tissue, looked for evidence of toxicity at doses higher than those required for neuroprotection, and confirmed the neuroprotective effect of NIr on dopaminergic cells in the substantia nigra pars compacta (SNc) in an acute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model of Parkinson disease in mice. Results The device was stable in freely moving animals, and the NIr filled the cranial cavity. Measurements showed that the NIr intensity declined as distance from the source increased across the brain (65% per mm) but was detectable up to 10 mm away. At neuroprotective (0.16 mW) and much higher (67 mW) intensities, the NIr caused no observable behavioral deficits, nor was there evidence of tissue necrosis at the fiber tip, where radiation was most intense. Finally, the intracranially delivered NIr protected SNc cells against MPTP insult; there were consistently more dopaminergic cells in MPTP-treated mice irradiated with NIr than in those that were not irradiated. Conclusions In summary, the authors showed that NIr can be applied intracranially, does not have toxic side effects, and is neuroprotective.

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Section: Neuroprotection Of Dopaminergic Cells In the Snc Via Nir Aftsupporting

confidence: 92%

Section: Neuroprotection Of Dopaminergic Cells In the Snc Via Nir Aftmentioning

confidence: 99%

Photobiomodulation inside the brain: a novel method of applying near-infrared light intracranially and its impact on dopaminergic cell survival in MPTP-treated mice

Moro

Massri²,

Torres

et al. 2014

JNS

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“…With SS the classical cellular biochemical mechanism of electron transfer is enhanced, whereas with laser the photochemical and photophysical mechanisms utilizing the wave particle dualism of electron and the importance of radiation energy during the process are suggested. (19) The photochemical absorption of radiation by chromophores in the mitochondria leads to the generation of extra electrochemical potential, a short term primary response, that produces a change in reduction-oxidation state of the respiratory chain. (20,21) The light quanta acts only as a trigger for cellular metabolic regulation and the photo signal transduction from the cytoplasm to the nucleus is not specific for light signals but includes the normal way of controlling cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

Laser Photons and Pharmacological Treatments in Wound Healing

Al-Watban

Andres

2000

LASER THERAPY

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The exploitation of photobiology in medicine has been of great interest to mankind. There is a growing interest in the use of lasers for treatment purposes because of the photochemical alterations induced in biomolecules by light energy. In this paper we present our data on laser biostimulation, the combination of pharmacological treatments Solcoseryl TM (SS) and Polygen TM (PG) with light therapy using in-vitro and in-vivo models. In-vitro experiments indicate the ability of laser photons and pharmacological agents SS or PG to augment or abate the cloning efficiency of various cell lines. In-vivo studies focused on the dosimetry of various laser wavelengths and the use of wound healing drugs and 632.8nm laser in wound healing. The application of pharmacological treatments combined with laser therapy reveals the utility of light-drug treatment combinations. Given the ever-increasing cost of medical care, the burden incurred on patients, caregivers and society, this line of research fulfills the increasing need to develop treatment methods that enhance wound healing, especially in situations involving resistance to healing. Key Words: Laser Therapy, wound healing, growth factors. IntroductionDuring the last decade, a growing interest in Photomedicine has emerged from the creative use of lasers, giving rise to the field of "Laser Biostimulation" or "Low Level Laser Therapy". This form of therapy is now widely used for the treatment of a variety of conditions, including wound healing, reduction of edema and pain of various etiologies.Wound healing photons and its dosimetric parameters have been studied extensively in our laboratory using various lasers: HeCd (442nm), Argon (488-514nm), HeNe (632.8nm), Krypton (670nm), GaAlAs (780nm and 830nm) and CO 2 (10,600nm).(1-4) Our studies suggest that HeNe 632.8nm is the best laser for biostimulation and that laser acceleration of wound healing cannot be attributed to laser skin transmission. (5) We determined the effect of 632.8nm laser in-vitro and invivo with SS, a calf hemodialysate and PG, a cocktail of growth factors and growth hormone (GH). Our in-vitro studies support the use of combined laser+SS (Al-Watban et al., in press) and laser+PG treatments in a nutrient deficient medium.(6) Our in-vivo studies on normal rats have shown that laser+SS or laser+PG minimally accelerate wound healing when compared to the single effect of 632.8nm laser (Al-Watban et al, in press). As a follow-up to our previous studies, we now present a report on a study in which we used an impaired wound healing model to further test the efficacy of laser+drug therapy.The need to promote wound healing, especially during a state of impairment, is imperative due to the ever-increasing cost of medical care and the burden imposed on patients, health care services and society, in general. Materials and Methods In-vivo ExperimentsAnimals: A number of male Sprague-Dawley rats were used in this study. The animals were originally imported from Charles River Co., UK in 1984. Now they are bred and provid...

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“…Esses fatores associados tendem a limitar a redução do limiar de excitabilidade dos receptores dolorosos (35) e eliminar substâncias alogênicas, por meio da modulação do metabolismo celular basal induzida pela endor ina que, em últi-ma instância, promoverá uma ação anti-in lamatória (36)(37)(38)(39). Uma possível explicação para a redução dos espasmos na musculatura da ATM tratada com laserterapia (40) pode estar associada aos níveis de energia absorvida nos citocromos e por irinas dentro da mitocôndria e membrana celular dessas células.…”

Section: Resultsunclassified

O uso do laser na reabilitação das desordens temporomandibulares

Assis

Soares²,

Victor³

2012

Fisioter. mov.

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ResumoIntrodução: Entende-se disfunção temporomandibular comoum termo coletivo que envolve um grande nú-mero de problemas clínicos que afetam os músculos mastigatórios, a articulação temporomandibular (ATM) e estruturas associadas. Dentre os principais sinais e sintomas, incluem-se dores nos músculos da mastigação ou na ATM, ruídos articulares, limitação de abertura, retração gengival, oclusão inadequada, distúrbios auditivos, cefaleias, sensibilidade em toda a musculatura do sistema estomatognático e cervical. Objetivos: Estudar a in luência da laserterapia de baixa intensidade (diodo) no tratamento das desordens temporomandibulares. Materiais e métodos: Trata-se de uma revisão sistemática. Foram consultadas as seguintes bases de dados: BIREME, MEDLINE, PubMed, Science Direct e LILACS. Os termos de busca utilizados foram "temporomandibular laser therapy" e "ATM laser treatment", em inglês e português. Não foram estabelecidos limites de busca. Após a busca independente de dois revisores, foram encontrados 1.344 artigos e, após a aplicação dos critérios de exclusão, 12 artigos foram selecionados para a análise. Resultados: Dos 12 artigos selecionados, apenas em 1 (um) deles não foi veri icada qualquer melhora do quadro doloroso na ATM em virtude dos diversos tipos de DTM. Conclusão: Apesar dos resultados satisfatórios do uso do laser na redução sintomática da dor nos DTM, ainda é controversa a utilização dos seus parâmetros para cada caso. Por isso, sugerimos a realização de outros estudos para que essas lacunas sejam preenchidas com o objetivo Fisioter Mov. 2012 abr/jun;25(2):453-9 Assis TO, Soares MS, Victor MM. 454 maior de promover uma melhor qualidade de vida para as pessoas que sofrem quaisquer transtornos na região da ATM. [P] Palavras-chave: Lasers semicondutores. Transtornos da articulação Temporomandibular.Modalidades de isioterapia.[B]Abstract Introduction: It is understood by temporomandibular dysfunction as a collective term that involves a large

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Import of Radiation Phenomena of Electrons and Therapeutic Low-Level Laser in Regard to the Mitochondrial Energy Transfer

Cited by 93 publications

References 2 publications

Photobiomodulation inside the brain: a novel method of applying near-infrared light intracranially and its impact on dopaminergic cell survival in MPTP-treated mice

Photobiomodulation inside the brain: a novel method of applying near-infrared light intracranially and its impact on dopaminergic cell survival in MPTP-treated mice

Laser Photons and Pharmacological Treatments in Wound Healing

O uso do laser na reabilitação das desordens temporomandibulares

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